Medicament-induced hemolytic anemia detection kit and detection method thereof

A technology of hemolytic anemia and detection kit, which is applied in the detection kit of drug-induced hemolytic anemia, and the field of detection of drug-induced hemolytic anemia, can solve the problem of reducing the efficacy of drugs, not carrying out the detection work of drug-induced hemolytic anemia, Problems such as destruction of red blood cells in patients

Inactive Publication Date: 2014-03-26
江苏中济万泰生物医药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason is that the detection of drug-induced hemolytic anemia belongs to the scope of immunohematology detection, while the immunohematology detection is only carried out in the blood transfusion department or blood bank of the clinical hospital. Therefore, patients with drug-induced hemolytic anemia cannot be detected and diagnosed in time, resulting in a fatality rate of more than 30%.
[0005] Drugs sometimes cause the formation of antibodies against the drug itself, and the formed drug antibodies can be sensitized or adsorbed on the patient's red blood cells, resulting in a positive test of the patient's red blood cells for direct antihuman globulin , causing the destruction of red blood cells in patients, resulting in immune hemolysis, and the decrease of hemoglobin in patients
The results are: 1. The efficacy of the drugs used to treat patients is reduced or inhibited
2. Patients with anemia due to drug-induced hemolysis
[0007] At present, there is no detection kit for detecting drug-induced hemolytic anemia, and there is no detection reagent that can detect whether immune hemolytic anemia is caused by the drug

Method used

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  • Medicament-induced hemolytic anemia detection kit and detection method thereof
  • Medicament-induced hemolytic anemia detection kit and detection method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The present invention detects drug-induced hemolytic anemia (test tube method) including:

[0032] 1. Drug diluent: including drug diluent I, drug diluent II, and drug diluent III.

[0033] 2. Antibody release reagents: including antibody release solution I, antibody release solution II, and antibody release solution III.

[0034] 3. Antibody screening reagent: broad-spectrum anti-human globulin reagent (anti-IgG+C 3 d).

[0035] Detection method:

[0036] 1. Preparation of drug-sensitized red blood cells:

[0037] (1) Preparation of drug solution: Take an appropriate amount of drug used by patients (such as penicillin) and dissolve it in drug diluent I (pH 9.6 barbiturate buffer) to make the drug concentration 1mg / ml.

[0038] (2) Wash 0.5 ml of normal human type O packed red blood cells with 0.9% sodium chloride solution three times to obtain packed red blood cells.

[0039] (3) The packed erythrocytes obtained in step (2) are mixed with 0.9% physiological sodium...

Embodiment 2

[0063] The present invention detects drug-induced hemolytic anemia (micro-column gel method) including:

[0064] 1. Drug diluent: including drug diluent I, drug diluent II, and drug diluent III.

[0065] 2. Antibody release reagents: including antibody release solution I, antibody release solution II, and antibody release solution III.

[0066] 3. Antibody screening reagents: Micro-column gel card includes broad-spectrum anti-human globulin reagents (anti-IgG+C 3 d) Gel column.

[0067] Detection method:

[0068] 1. Preparation of drug-sensitized erythrocytes: same as in Example 1, the cell concentration was adjusted to 0.5-1%.

[0069] 2. Preparation of antibody release solution: same as in Example 1.

[0070] 3. Detection of drug-induced hemolytic anemia using drug-sensitized red blood cells:

[0071] (1) Take broad-spectrum anti-human globulin reagent (anti-IgG+C 3 d) Micro-column gel card, mark 6 wells, respectively "1~6". Add 50 μl of 0.5-1% drug-sensitized red blo...

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Abstract

The invention discloses a medicament-induced hemolytic anemia detection kit and also discloses a detection method using the kit. When plasma and antibody release liquid of a patient generate a positive reaction with corresponding medicament-sensitized erythrocytes, the plasma and the antibody release liquid of the patient generate a negative reaction with unsensitized erythrocytes; or a medicament is added into the plasma, the plasma or medicament antibodies in the antibody release liquid generates a positive reaction with normal erythrocytes, the plasma which is not added with the medicament or a dispersion solution generate a negative reaction with the normal erythrocytes. The medicament species causing the hemolytic anemia can be diagnosed accurately according to the results so as to guide to correctly select appropriate medicaments for treating the patient in clinic. The method has high accuracy, and is applicable to the detection by a test tube method or a microcolumn gel card method.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a drug-induced hemolytic anemia detection kit, and also relates to a method for using the kit to detect drug-induced hemolytic anemia. Background technique [0002] With the large increase in the variety of drugs, especially the large-scale use of antibiotics in clinical practice, the cases of drug-induced hemolytic anemia have increased significantly. Due to immune factors, a large number of red blood cells have been destroyed, and hemolysis such as anemia, jaundice, and soy sauce-colored urine have occurred clinically. Symptoms, ranging from affecting the treatment effect, seriously delaying the treatment and endangering the life safety of patients. [0003] How to avoid drug-induced hemolytic anemia in patients due to drug use, it is necessary to carry out timely and accurate detection of drug-induced hemolytic anemia in patients, stop using drugs that can cause drug-induce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/5008G01N33/94G01N2500/10G01N2800/22
Inventor 周珍祯陈芳芳
Owner 江苏中济万泰生物医药有限公司
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