Medicament-induced hemolytic anemia detection kit and detection method thereof

Abstract

The invention discloses a medicament-induced hemolytic anemia detection kit and also discloses a detection method using the kit. When plasma and antibody release liquid of a patient generate a positive reaction with corresponding medicament-sensitized erythrocytes, the plasma and the antibody release liquid of the patient generate a negative reaction with unsensitized erythrocytes; or a medicament is added into the plasma, the plasma or medicament antibodies in the antibody release liquid generates a positive reaction with normal erythrocytes, the plasma which is not added with the medicament or a dispersion solution generate a negative reaction with the normal erythrocytes. The medicament species causing the hemolytic anemia can be diagnosed accurately according to the results so as to guide to correctly select appropriate medicaments for treating the patient in clinic. The method has high accuracy, and is applicable to the detection by a test tube method or a microcolumn gel card method.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a drug-induced hemolytic anemia detection kit, and also relates to a method for using the kit to detect drug-induced hemolytic anemia. Background technique [0002] With the large increase in the variety of drugs, especially the large-scale use of antibiotics in clinical practice, the cases of drug-induced hemolytic anemia have increased significantly. Due to immune factors, a large number of red blood cells have been destroyed, and hemolysis such as anemia, jaundice, and soy sauce-colored urine have occurred clinically. Symptoms, ranging from affecting the treatment effect, seriously delaying the treatment and endangering the life safety of patients. [0003] How to avoid drug-induced hemolytic anemia in patients due to drug use, it is necessary to carry out timely and accurate detection of drug-induced hemolytic anemia in patients, stop using drugs that can cause drug-induce...

AI Technical Summary

Problems solved by technology

The reason is that the detection of drug-induced hemolytic anemia belongs to the scope of immunohematology detection, while the immunohematology detection is only carried out in the blood transfusion department or blood bank of the clinical hospital. Therefore, patients with drug-induced hemolytic anemia cannot be detected and diagnosed in time, resulting in a fatality rate of more than 30%.

[0005] Drugs sometimes cause the formation of antibodies against the drug itself, and the formed drug antibodies can be sensitized or adsorbed on the patient's red blood cells, resulting in a positive test of the patient's red blood cells for direct antihuman globulin , causing the destruction of red blood cells in patients, resulting in immune hemolysis, and the decrease of hemoglobin in patients

The results are: 1. The efficacy of the drugs used to treat patients is reduced or inhibited

2. Patients with anemia due to drug-induced hemolysis

[0007] At present, there is no detection kit for detecting drug-induced hemolytic anemia, and there is no detection reagent that can detect whether immune hemolytic anemia is caused by the drug

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