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Content detection method of D-sodium erythorbate

A sodium erythorbate and detection method technology, which is applied in the field of content detection of D-sodium erythorbate, achieves the effects of simple operation, low detection cost and good reproducibility

Active Publication Date: 2015-04-08
CHINA RESOURCES CESTBON BEVERAGE CHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no mature national standard for the determination of D-isoascorbate sodium. Only the Jilin Provincial Department of Health issued the local standard for the determination method of D-isovitamin C sodium in food in December 2012. Separation column, acetonitrile-0.1% phosphoric acid aqueous solution as mobile phase determination

Method used

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  • Content detection method of D-sodium erythorbate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Chromatographic conditions and system suitability test:

[0022] Agilent 1260Infinity High Performance Liquid Chromatography (Agilent, USA); Agilent Diode Array Detector; Chromatographic Column: PROSHELL120EC-C18 (3.0×50mm2.7 Micron); Mobile Phase: Potassium Dihydrogen Phosphate Solution with a concentration of 0.8% and a pH of 4.0 - Methanol, the volume ratio of the two components is (80:20);

[0023] 2. Selection of detection wavelength

[0024] Accurately weigh 3mg of D-sodium erythorbate reference substance, place it in a 100mL volumetric flask, add pure water to fully dissolve, dilute to the mark, shake well, filter, and use it as the reference substance solution. Use the Agilent autosampler to precisely draw 5 μL and inject it into the liquid chromatograph, and the chromatogram of the component is obtained by the Agilent diode array detector (attached figure 1 ) and UV spectra. From the results of the chromatogram and the ultraviolet spectrogram, it can be s...

Embodiment 2

[0032] 1. Chromatographic conditions and system suitability test:

[0033] Agilent 1260 Infinity High Performance Liquid Chromatography (Agilent, USA); Agilent Diode Array Detector; Chromatographic Column: PROSHELL120EC-C18 (3.0×50mm2.7 Micron); Mobile Phase: Potassium Dihydrogen Phosphate Solution with a concentration of 0.6% and a pH of 4.0 - Methanol, the volume ratio of the two components is (95:5);

[0034] 2. Selection of detection wavelength

[0035] Accurately weigh 3mg of D-sodium erythorbate reference substance, place it in a 100mL volumetric flask, add pure water to fully dissolve, dilute to the mark, shake well, filter, and use it as the reference substance solution. Use the Agilent autosampler to precisely draw 5 μL and inject it into the liquid chromatograph, and the chromatogram of the component is obtained by the Agilent diode array detector (attached figure 1 ) and UV spectra. From the results of the chromatogram and the ultraviolet spectrogram, it can be s...

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Abstract

The invention relates to a content detection method of D-sodium erythorbate. Octadecylsilane chemically bonded silica is used as chromatographic column filling material; potassium dihydrogen phosphate solution-methanol is used as mobile phase; an ultraviolet detector is used for detection. Chromatographic peaks of D-sodium erythorbate and other components in the tea beverage and other sample are baseline resolved symmetrical Gaussian peaks with good peak patterns, thereby satisfying qualitative and quantified requirements; According to the invention, the chromatogram condition has few damages to the used chromatographic column, so the chromatographic column life is longer and the detection cost is lower in normal usage condition; the detection method of the invention has the advantages of accuracy, simple operation, good reappearance, high sensitivity and good practicality in work.

Description

technical field [0001] The invention relates to a method for detecting the content of sodium D-isoascorbate. Background technique [0002] D-sodium erythorbate, scientific name: D-2,3,5,6-tetrahydroxy-2-ethylene acid-γ-lactone sodium salt, also known as sodium erythorbate, sodium erythorbate. The molecular formula is: C 6 h 7 NaO 6 ·H 2 O. The product is white powder or granules, odorless, slightly salty, soluble in water. [0003] Sodium D-erythorbate, as an antioxidant commonly added in the current beverage industry, protects the color of the contents. Because it is easy to be oxidized, it is easy to be oxidized and attenuated by the dissolved oxygen in the content, the oxygen in the headspace of the container or the oxygen infiltrated through the packaging during the storage of the beverage. When its content is attenuated to a certain level, it will not be able to With its color protection effect, the content will change color, which will affect the sensory percept...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 张志刚郑羽茜
Owner CHINA RESOURCES CESTBON BEVERAGE CHINA CO LTD