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Method for measuring saponin content in lychee seed extract

A technology of extract and litchi core, applied in the field of determination of saponin content in litchi core extract, can solve the problems of high measured value, failure to reflect the real content of saponin, unfavorable quality control of extract, etc., and achieve strong stability, quality control and Medication safety assurance, good reproducible effect

Active Publication Date: 2014-04-02
GUANGZHOU HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, adopting this method to measure the saponin content of the litchi seed extract will make the measured value on the high side, which cannot reflect the true content of saponin in the extract, and is not conducive to the quality control of the extract.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Instrument:

[0018] Agilent1100 High Performance Liquid Chromatography (Agilent, USA), including degasser, quaternary gradient pump, autosampler, column thermostat and HP Chemstation workstation; Allteeh ELSD2000 detector (Allteeh, USA); AE240 electronic Analytical balance (Mettler-Toledo Instrument Co., Ltd.); Buchi rotary evaporator (Buchi, Switzerland); KQ3200B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0019] 2. Test drug:

[0020] Litchi seed extract (self-made according to the method of patent CN102048885); ginsenoside Rg1 reference substance (China Institute for Food and Drug Control); n-butanol (analytical grade); methanol (chromatographic grade).

[0021] 3. Chromatographic conditions:

[0022] The column is Agilent Zorbax Eclipse XDB-C 18 Column, specifications: 4.6mm×250mm, 5μm; mobile phase is methanol-water (70:30), flow rate is 1.0mL / min, column temperature is 30°C; detector is evaporative light scattering detector, detection pa...

Embodiment 2

[0037] 1. Instrument:

[0038] Agilent1100 High Performance Liquid Chromatography (Agilent, USA), including degasser, quaternary gradient pump, autosampler, column thermostat and HP Chemstation workstation; Allteeh ELSD2000 detector (Allteeh, USA); AE240 electronic Analytical balance (Mettler-Toledo Instrument Co., Ltd.); Buchi rotary evaporator (Buchi, Switzerland); KQ3200B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0039] 2. Test drug:

[0040] Litchi seed extract (self-made according to the method of patent CN102048885); ginsenoside Rg1 reference substance (China Institute for Food and Drug Control); n-butanol (analytical grade); methanol (chromatographic grade).

[0041] 3. Chromatographic conditions:

[0042] The column is Agilent Zorbax Eclipse XDB-C 18 Column, specifications: 4.6mm×250mm, 5μm; mobile phase is methanol-water (70:30), flow rate is 1.0mL / min, column temperature is 30°C; detector is evaporative light scattering detector, detection para...

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PUM

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Abstract

The invention provides a method for measuring saponin content in a lychee seed extract. The method comprises steps as follows: (1), preparing a reference substance solution; (2), preparing a test article solution; (3), precisely absorbing a ginsenoside reference substance solution for sample introduction, and measuring a peak area to obtain a curvilinear equation; and (4), precisely absorbing the test article solution for sample introduction, measuring a peak area, and calculating the saponin content in a sample according to a standard curve. The method is based on high performance liquid chromatography-coupled with evaporative light scattering detection and has good specificity, reproducibility and stability, so that quality control and medication safety of a product can be guaranteed.

Description

technical field [0001] The invention belongs to the field of medicine quality control, in particular to a method for measuring saponin content in litchi seed extract. Background technique [0002] Lychee Seed, Litchi Seed, is the dry mature seed of Sapindaceae plant Litchi (Litchi Chinensis Sonn), aliased as Li Ren, Li He, Da Li He, included in the 2010 edition of Chinese Pharmacopoeia, Warm in nature, sweet in taste, slightly bitter, has the effects of promoting qi and dispelling stagnation, dispelling cold and relieving pain. "Compendium of Materia Medica" records that lychee cores "quench thirst, benefit people's color, and eat them to relieve polydipsia" (polydipsia, modern medicine is called diabetes). Studies have shown that lychee core has the functions of lowering blood sugar, regulating blood lipids, improving anti-oxidation, and inhibiting the surface antigen of hepatitis B virus. The ingredients contained in lychee kernels have been well studied. The main ingred...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 郭洁文邓志军屈喜玲
Owner GUANGZHOU HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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