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A rapid detection kit for chicken infectious bronchitis virus

A detection kit and bronchitis technology, applied in the field of chicken infectious bronchitis virus detection, can solve the problems of easy cross-contamination by RT-PCR, expensive thermal cycle equipment, poor stability, etc., and achieve good reproducibility and high accuracy , the effect of high sensitivity

Active Publication Date: 2015-09-09
SHANDONG BINZHOU BOLAIWEI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the detection methods for chicken infectious bronchitis virus are time-consuming, complicated to operate, poor in sensitivity and stability
The PCR method has the advantages of strong specificity, high sensitivity, and fast speed, but it is difficult to be popularized due to the disadvantages of RT-PCR, such as easy cross-contamination, cumbersome operation, and expensive thermal cycle equipment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A rapid detection kit for chicken infectious bronchitis virus of the present invention comprises: chicken infectious bronchitis N recombinant protein coated microwell reaction plate / strip, stop solution, positive control and negative control, enzyme conjugate, sample dilution Solution, Concentrated Washing Solution, Chromogenic Solution A and Chromogenic Solution B.

[0019] The preparation method of chicken infectious bronchitis N recombinant protein coated microwell reaction plate / strip is as follows: use 0.1mol / L Tris-HCl buffer solution of pH 8 as the coating solution, dilute chicken infectious bronchitis N recombinant protein to 5 μg / mL, add 50 μL / well to the ELISA reaction plate, block at 37°C for 2 hours, coat at 4°C overnight, pat dry, block with 1% bovine serum albumin at 37°C for 3 hours, wash with 20% NaCl solution containing Tween-20 , pat dry, then add 20% sucrose phosphate buffer and keep at room temperature for 2h.

[0020] The sample diluent is a 10% N...

Embodiment 2

[0028] A rapid detection kit for chicken infectious bronchitis virus of the present invention comprises: chicken infectious bronchitis N recombinant protein coated microwell reaction plate / strip, stop solution, positive control and negative control, enzyme conjugate, sample dilution Solution, Concentrated Washing Solution, Chromogenic Solution A and Chromogenic Solution B.

[0029] The preparation method of chicken infectious bronchitis N recombinant protein coated microwell reaction plate / strip is as follows: use 0.1mol / L Tris-HCl buffer solution of pH 8 as the coating solution, dilute chicken infectious bronchitis N recombinant protein to 5 μg / mL, add 50 μL / well to the ELISA reaction plate, block at 37°C for 2 hours, coat at 4°C overnight, pat dry, block with 1% bovine serum albumin at 37°C for 2 hours, wash with 20% NaCl solution containing Tween-20 , pat dry, then add 20% sucrose phosphate buffer and keep at room temperature for 2h.

[0030] The sample diluent is a 10% N...

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PUM

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Abstract

The invention discloses a kit for rapidly detecting infectious bronchitis viruses. The kit comprises an infectious bronchitis virus N-recombined protein enveloped microporous reaction plate / strip, terminating liquid, a male contrast, a female contrast, an enzyme composition, a sample diluted solution, concentrated washing liquid, a color-developing solution A and a color-developing solution B. The kit is applied to large-batch detection and one-by-one / group-by-group manual detection and has the characteristics of high sensitivity and accuracy and high reproducibility.

Description

technical field [0001] The invention relates to the technical field of chicken infectious bronchitis virus detection, in particular to a rapid detection kit for chicken infectious bronchitis virus. Background technique [0002] Avian Infectious Bronchitis (IB) is an acute, highly contagious infectious disease of chickens caused by Avian Infectious Bronchitis Virus (IBV) of the genus Coronaviridae in the family Coronaviridae. It mainly affects the respiratory tract, digestive tract, reproductive system and kidney of chickens, and IBV has different degrees of harm to chickens of different ages. It is characterized by respiratory symptoms, decreased egg production and quality, and kidney lesions in sick chickens. IBV also often causes mixed infections (such as Newcastle disease, chronic respiratory disease, etc.), and can cause secondary bacterial diseases such as colibacillosis, salmonellosis, etc., thereby aggravating the harm to chickens. [0003] IBV nucleocapsid protein,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983G01N33/6875G01N2333/165
Inventor 陈申秒马景霞贾红梅董新荣吴雪莉
Owner SHANDONG BINZHOU BOLAIWEI BIOTECH