Colloidal gold test strip for rapidly detecting eperythrozoon suis in plasma

Abstract

The invention provides a colloidal gold test strip for rapidly detecting eperythrozoon suis in plasma. The colloidal gold test strip comprises a support layer arranged at the bottom layer, and an absorption layer arranged at the middle layer, wherein the absorption layer is fixed on the support layer; the absorption layer is sequentially provided with a sample absorption fiber layer, a red blood cell filter film, a gold-labeled antibody fiber layer, a cellulose membrane layer and a water absorbing material layer from a test end. A detection line coated by a monoclonal antibody of anti-eperythrozoon suis and a quality control line coated with a goat anti-mouse IgG antibody or a rabbit anti-mouse IgG antibody are arranged in the middle of the cellulose membrane layer; after the structure is adopted, the colloidal gold test strip has the advantages of being high in specificity, simple, convenient and fast to operate, visual and accurate in result display wide in application scope, and the like, and the investment and the detection cost are reduced, and thus the colloidal gold test strip can be widely applied to detecting the eperythrozoon suis in basic level.

Description

technical field [0001] The invention relates to the field of preventive veterinary examination, in particular to a colloidal gold test strip for rapid detection of Eperythrozoon porcine. Background technique [0002] Eperythrozoonosis is a zoonotic disease caused by Mycoplasma suis (M.suis) parasitizing on the surface of pig red blood cells, plasma, bone marrow and cerebrospinal fluid. It is generally a recessive infection. The infection rate in pig herds is as high as 90% or more, and the symptoms such as anemia, jaundice, fever, swollen lymph nodes, and abortion of sows are generally manifested in acute onset. Eperythrozoon is classified as Rickettsia in Bergey's manual, but it is generally believed that it belongs to mycoplasma according to the research on its 16sRNA sequence. Eperythrozoon was discovered in rodents by Schilling and Dinger in 1928; Doyle et al. (1932) reported Eperythrozoon disease in pigs in India in 1932; in 1950, Splitter (1950) officially named the d...

AI Technical Summary

Problems solved by technology

At present, the main methods for the detection of M. suis are: (1) Using blood microscopic examination technology: clinically, the method of collecting porcine peripheral blood pressure drop slides or blood picture staining microscopic examination is used to detect the M. suis in plasma and on red blood cells. Erythrozoon, but the accuracy and sensitivity of this method are low, and the subjectivity is high; (2) Enzyme-linked immunosorbent assay (ELISA) is used to detect specific antibodies in serum. Sensitive, but due to the need for a large number of reagents and instruments, and the need for professional and technical personnel to operate and other factors, this method is not easy to popularize and apply, especially not suitable for field application by grassroots personnel

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