mPEG (methoxy polyethylene glycol) grafted alpha-zein polymer and preparation method thereof
A zein and polymer technology, applied in the field of polymers, can solve the problems of unfavorable drug release and uniform particle size distribution
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[0035] Preparation example of α-zein
[0036] 1 part of corn flour was dissolved in 5 parts of 50-75% ethanol, and the supernatant was obtained by centrifugation. Dilute with water until there is no more protein precipitation, filter and dry to obtain zein. Use n-hexane and other solvents to remove the grease in zein, take 1 part of defatted zein and dissolve it in 30 parts of N-methylpyrrolidone, and extract repeatedly 3 times in 90 parts of dichloromethane to remove riboflavin. Finally, it was washed with more than 90% ethanol solution, centrifuged to take the supernatant, diluted with water to a concentration below 80%, and filtered; the filtered solid was washed with n-hexane, and vacuum-dried to obtain white α-zein powder.
Embodiment 1
[0038] 1g mPEG (Mz=1KDa) was dissolved in dichloromethane, and excess succinic anhydride was added. 45°C water bath, stirring at 300rpm for 5h. Precipitation was repeated twice in diethyl ether and dichloromethane, and vacuum overnight after suction filtration. After dissolving the obtained white powder in water, inject it into a dialysis bag with a relative molecular weight of 3500, and perform dialysis for two days. mPEG-COOH was obtained after lyophilization. Dissolve the above product in 10ml of N-methylpyrrolidone, add 0.018g EDC·HCl (activator) and 0.027g NHS, stir and activate for 3h. Slowly add the above mixed solution to 1g of α-zein (Mz=26KDa) in dimethyl sulfoxide solution, stir for 28 hours, after the reaction is completed, precipitate in ether, then dissolve in N-methylpyrrolidone, repeat 2 Second-rate. The product obtained by drying was dissolved in water, poured into a dialysis bag with a molecular weight cut off of 30,000, dialyzed in water for 1 day, and f...
Embodiment 2
[0042] 10g mPEG (Mz=25KDa) was dissolved in dichloromethane, and excess succinic anhydride was added. 65°C water bath, stirring at 700rpm for 3h. Precipitate in diethyl ether repeatedly 4 times, vacuum overnight after suction filtration. After dissolving the obtained white powder in water, inject it into a dialysis bag with a relative molecular weight of 35,000, and perform dialysis for two days. mPEG-COOH was obtained after lyophilization. Dissolve the above product in 100ml N-methylpyrrolidone, add 0.036g EDC·HCl (activator) and 0.054g NHS, stir and activate for 4h. Slowly add the above mixed solution dropwise to 1.2g of α-zein (Mz=22KDa) in dimethyl sulfoxide solution, stir for 24 hours, after the reaction is completed, precipitate in ether, then dissolve in N-methylpyrrolidone, repeat 4 times. The product obtained by drying was dissolved in water, injected into a dialysis bag with a molecular weight cut off of 35,000, dialyzed in water for 4 days, and freeze-dried to o...
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