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A portable glucose test tube

A glucose and test tube technology, applied in the field of glucose detection, can solve the problems of complex detection process, large background interference, and high operation requirements, and achieve the effects of low detection cost, good reproducibility, and easy portability

Active Publication Date: 2015-12-02
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a portable test tube for glucose detection, the principle of which is to use hydrogel as a carrier, based on molybdenum disulfide as the glucose color reaction detection method of the simulated enzyme, which can solve the problem of glucose detection in the prior art High operating requirements, complex detection process, high detection cost, long detection time, large background interference and other problems, and the glucose test tube of the present invention is low in cost, easy to operate, can visualize and quickly detect glucose in samples, and is easy to carry and store

Method used

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  • A portable glucose test tube

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preparation example Construction

[0015] The preparation method mainly includes the following steps: adding molybdenum disulfide solution, chromogen and glucose oxidase solution into the hydrogel solution, mixing evenly, and then transferring the mixed solution to a small groove inside the cover of the centrifuge tube. Leave it for 5 minutes under the condition to make it solidify into a gel, and then the glucose test tube is obtained.

[0016] The hydrogel includes agar hydrogel, agarose hydrogel or chitosan hydrogel.

[0017] The developer includes 3,3',5,5'-tetramethylbenzidine, 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt or o- phenylenediamine.

[0018] The detection method is to dilute the glucose sample and add it to the bottom of the test tube, cover the lid and turn it upside down, let the sample solution contact with the gel for a period of time, and then semi-quantitatively detect the glucose concentration according to the color change of the gel.

Embodiment 1

[0020] Preparation method of portable glucose test tube: 200μL molybdenum disulfide solution (18mg / L), 300μL 3,3',5,5'-tetramethylbenzidine solution (12mM) and 100μL glucose oxidase solution (10mg / mL) Add to 2mL agarose (10mg / mL) hydrogel solution, mix well at 40°C, then transfer 150μL of the mixed solution to the small groove inside the lid of the centrifuge tube, and place it under natural conditions for 5min to solidify After forming a gel, the glucose test tube was obtained and stored at 4°C.

[0021] Detection of glucose in serum: Dilute the serum sample 30 times, add 500 μL to the bottom of the test tube, cover the lid and turn over the test tube, place it at 37°C for 30 minutes, turn over the centrifuge tube to separate the gel from the sample solution, The content of glucose in the serum was detected by the color change of the hydrogel.

Embodiment 2

[0023] The preparation method of the portable glucose test tube: mix 200μL molybdenum disulfide solution (18mg / L), 300μL 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (20mM) and Add 100 μL of glucose oxidase solution (10 mg / mL) to 2 mL of chitosan (10 mg / mL) hydrogel solution, mix well at 40 ° C, then transfer 150 μL of the mixed solution to the small groove inside the centrifuge tube lid, Place it under natural conditions for 5 minutes to solidify into a gel to obtain a glucose test tube and store it at 4°C.

[0024] Detection of glucose in serum: Dilute the serum sample 30 times and add 500 μL to the bottom of the test tube, cover the lid and turn over the test tube, place it at 37°C for 40 minutes, turn over the centrifuge tube to separate the gel from the sample, pass The color change of the hydrogel detects the content of glucose in the serum.

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Abstract

The invention discloses a portable test tube for detecting glucose. A hydrogel is used as a carrier, the glucose is oxidized by glucose oxidase and generated into hydrogen peroxide, and then, a glucose sample is detected through the chromogenic reaction, which is catalyzed by molybdenum disulfide, between the hydrogen peroxide and a color developing agent. The problems in the prior art, such as high requirements on glucose detecting operation, complicated detection process, high detecting cost, long detecting time, big background interference can be solved. The glucose test tube has the advantages of low cost, simplicity in operation, capability of visually and rapidly detecting the glucose content in the sample, and convenience in carry and storage.

Description

technical field [0001] The invention belongs to the technical field of glucose detection, in particular to a portable test tube for detecting glucose. Background technique [0002] In recent years, due to the influence of many factors such as changes in diet structure, increasingly tense life rhythm and less active and more sedentary lifestyle, the incidence of diabetes worldwide has increased rapidly, making diabetes the third serious threat after tumors and cardiovascular diseases Chronic diseases of human health. The detection of glucose in serum is the main means of clinical examination and monitoring of diabetes. At present, most of the device inventions for detecting glucose are electrochemical sensors (patent number: 01810317.0; utility model patent: 200620110322.2; patent number: 201210116431.5; patent number: 201210189645.5; patent number: 201210416267.X; patent number: 201310087177.5; ). The core of these devices is the preparation of functionalized electrodes, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 郭良洽林天然
Owner FUZHOU UNIV