Novel method for assembling multi-layer biological enzyme sensor to improve detection performance by using graphene
A graphene and sensor technology, applied in the field of electrochemical analysis research
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[0017] Example 1. (1) Use pyrenebutyric acid to modify the biological enzyme (glucose oxidase as an example) to have a large π ring end group on the surface: take 5 mg of glucose oxidase, 1-(3-dimethyl Aminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) 10 mg, N-hydroxysuccinimide (NHS) 2 mg were dissolved in 9.5 mL pH=7.4 phosphate buffer solution. Dissolve 2 mg of pyrenebutyric acid in 1 mL of N,N-dimethylformamide (DMF). Slowly add 0.5 mL of pyrenebutyric acid solution to the glucose oxidase solution, and stir at room temperature for 10 hours. After the reaction, remove unreacted small molecules by dialysis in phosphate buffer solution to obtain pyrenebutyric acid-modified Glucose oxidase.
[0018] (2) Preparation of graphene: Oxidation of graphite powder: Add 2 g of graphite powder, 1 g of potassium persulfate, and 1 g of phosphorus pentoxide to 3 ml of concentrated sulfuric acid, and obtain a dark blue liquid after 6 hours. Dilute with distilled water, filter, wash, an...
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