Novel method for assembling multi-layer biological enzyme sensor to improve detection performance by using graphene

A graphene and sensor technology, applied in the field of electrochemical analysis research

Inactive Publication Date: 2014-04-23
QINGDAO UNIV
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Problems solved by technology

The modified enzyme and graphene are assembled layer by layer through π-π superposition, thus solving the problem of biosensor assembly with controllable layer number, orderly structure and stable performance, which can greatly improve the detection sensitivity and performance of the glucose oxidase electrode. examination range

Method used

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  • Novel method for assembling multi-layer biological enzyme sensor to improve detection performance by using graphene
  • Novel method for assembling multi-layer biological enzyme sensor to improve detection performance by using graphene
  • Novel method for assembling multi-layer biological enzyme sensor to improve detection performance by using graphene

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Embodiment 1

[0017] Example 1. (1) Use pyrenebutyric acid to modify the biological enzyme (glucose oxidase as an example) to have a large π ring end group on the surface: take 5 mg of glucose oxidase, 1-(3-dimethyl Aminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) 10 mg, N-hydroxysuccinimide (NHS) 2 mg were dissolved in 9.5 mL pH=7.4 phosphate buffer solution. Dissolve 2 mg of pyrenebutyric acid in 1 mL of N,N-dimethylformamide (DMF). Slowly add 0.5 mL of pyrenebutyric acid solution to the glucose oxidase solution, and stir at room temperature for 10 hours. After the reaction, remove unreacted small molecules by dialysis in phosphate buffer solution to obtain pyrenebutyric acid-modified Glucose oxidase.

[0018] (2) Preparation of graphene: Oxidation of graphite powder: Add 2 g of graphite powder, 1 g of potassium persulfate, and 1 g of phosphorus pentoxide to 3 ml of concentrated sulfuric acid, and obtain a dark blue liquid after 6 hours. Dilute with distilled water, filter, wash, an...

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Abstract

The invention aims at providing a novel method for assembling a multi-layer biological enzyme sensor to improve detection performance by using graphene. In order to utilize and transform a reagent pyrene butyric acid with a large phi-ring terminal group by amidation reaction with an amino group on the surface of biological enzyme (for example, glucose oxidase), so as to obtain the glucose oxidase with the large phi-ring terminal group on the surface, the transformed glucose oxidase is fixed on the surface of an electrode by using graphene in a layer-by-layer assembly manner, so as to prepare the biological enzyme sensor with controllable layer quantity and high sensitivity. The modified enzyme and the graphene are assembled layer by layer by phi-phi superposition. Thus, the assembly problem of the biological sensor with controllable layer quantity, ordered structure and stable performance is solved, and the detection sensitivity and the detection range of the biological enzyme sensor can be greatly improved.

Description

technical field [0001] The method relates to a preparation method of a new type of biological enzyme biosensor. We connect a reagent pyrenebutyric acid with a large π-ring end group to the surface of the biological enzyme through the amino group on the surface of the biological enzyme to make it modified. The modified biological enzyme (such as glucose oxidase) is immobilized on the surface of the electrode by layer-by-layer assembly method using graphene, and a biological enzyme sensor with controllable layer number and high sensitivity is prepared, which belongs to the research field of electrochemical analysis. Background technique [0002] Bioenzyme sensor is a special kind of chemical sensor. It uses biological enzyme as the biological sensitive element, captures the reaction between the target object and the sensitive element through various physical and chemical signal converters, and then uses discrete Or continuous electrical signal expression, it is a kind of detec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
Inventor 不公告发明人
Owner QINGDAO UNIV
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