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A kind of microbacterium chocolate magnetic cell and its preparation method and application

A microbacterium and chocolate technology, applied in the field of microbacteria chocolate magnetic cells and its preparation, can solve the problems of weak binding force between cells and materials, inability to reuse, low stability, etc., and achieve convenient separation, enzyme activity maintenance, and low activity Effect

Inactive Publication Date: 2016-06-22
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] One of the purposes of the present invention is to provide a kind of microbacterium chocolate in order to solve the above-mentioned technical problems such as weak binding force between cells and materials, low stability, easy to fall off in the batch reaction process of repeated use and unable to be reused. magnetic cell

Method used

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  • A kind of microbacterium chocolate magnetic cell and its preparation method and application
  • A kind of microbacterium chocolate magnetic cell and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A magnetic microbacterium chocolatier cell is specifically prepared by a method comprising the following steps:

[0042] (1), Chitosan-wrapped Fe 3 o 4 Preparation of Magnetic Nanomaterials

[0043] Dissolve 0.2g of chitosan (viscosity 400-600mPa.s) in 20ml of acetic acid aqueous solution with a mass percent concentration of 5%. After the chitosan is completely dissolved, add Fe 3 o 4 Particles 0.2g, ultrasonically mixed for 20 minutes to obtain a mixture;

[0044] Then, under strong stirring in a water bath at 40°C, disperse the resulting mixed solution in a three-necked flask filled with 40ml of liquid paraffin and 5ml of Span 80, stir for 30min, add 3ml of glutaraldehyde aqueous solution with a concentration of 25% by mass, and mechanically stir After reacting for 4 hours, the chitosan-coated Fe 3 o 4 Crude magnetic nanomaterials;

[0045] The obtained chitosan-coated Fe was fully ultrasonically washed with petroleum ether 3 o 4 The crude magnetic nanomateri...

Embodiment 2

[0057] A microbacterium chocolatier magnetic cell, specifically prepared by the method comprising steps as described in Example 1:

[0058] Just the Microbacterium chocolatier cells used in step (3) and the chitosan-coated Fe 3 o 4 The weight ratio and stirring time of magnetic nanomaterial are different from Example 1, and others are all the same as Example 1;

[0059] The used Microbacterium chocolatier cells with chitosan-coated Fe 3 o 4 The weight ratio of magnetic nanomaterials: that is, the used chitosan-wrapped Fe 3 o 4 Amount of magnetic nanomaterials, Microbacterium chocolate cells, by chitosan-coated Fe 3 o 4 The wet weight of magnetic nanomaterials: the dry weight of Microbacterium chocolate cells: the ratio of phosphate buffer solution is 150g: 10g: 1L;

[0060] Stirring time is 1h.

[0061] Others are the same as embodiment 1.

[0062] The immobilization rate of the magnetic cells of Microbacterium chocolate was calculated by the formula of immobilization...

Embodiment 3

[0064] A microbacterium chocolatier magnetic cell, specifically prepared by the method comprising steps as described in Example 1:

[0065] Just the Microbacterium chocolatier cells used in step (3) and the chitosan-coated Fe 3 o 4 The weight ratio and stirring time of magnetic nanomaterial are different from Example 1, and others are all the same as Example 1;

[0066] The used Microbacterium chocolatier cells with chitosan-coated Fe 3 o 4 The weight ratio of magnetic nanomaterials: that is, the used chitosan-wrapped Fe 3 o 4 Amount of magnetic nanomaterials, Microbacterium chocolate cells, by chitosan-coated Fe 3 o 4 The wet weight of magnetic nanomaterials: the dry weight of Microbacterium chocolate cells: the ratio of phosphate buffer solution is 150g: 10g: 1L;

[0067] Stirring time is 3h.

[0068] Others are the same as embodiment 1.

[0069] The immobilization rate of the magnetic cells of Microbacterium chocolate was calculated by the formula of immobilization...

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Abstract

The present invention discloses a chocolate microbacteria magnetic cell and its preparation methods and applications.The chocolate microbacterial cell suspension of the FE3O4 magnetic nanogenic material obtained by the obtained shell polycanic is costed by the chocolate microbacteria cells, the control temperature is 30 ° C, and the chocolate microorpus magnetic cells are stirled and adsorbed for 10-600 minutes.The chocolate microaczobacteria magnetic cells are used to catalyze the internal anti -rotation dihydride asymmetric hydrolyzed reactions (4 S 5 R ) -Memodide, can be repeatedly used, and after 10 times, chocolate microbacteria magnetic cells have more than 70%of the catalytic transformation rate of bioinomodox esters for substrates.This chocolate microorpur magnetic cell preparation method is simple, enzyme activity recovery rate is high, and stability is good, which can be convenient and quickly recovered and reused in the magnetic field.

Description

technical field [0001] What the present invention relates to is a kind of Microbacterium chocolate magnetic cell and its preparation method and application, more specifically relate to a kind of using chitosan-Fe 3 o 4 Microbacterium chocolate magnetic cells prepared by immobilizing Microbacterium chocolate with magnetic nanoparticles and its ability to catalyze the generation of asymmetric hydrolysis of meso-dimethyl ester (4 S ,5 R )-application in half esters. Background technique [0002] Cell immobilization refers to a basic technology that uses chemical or physical means to locate free cells in a limited space and keep them active and reusable. [0003] Compared with free cells, immobilized cells have significant advantages. For example, immobilized cells can have higher stability, can be reused, and the immobilization method is simpler and lower in cost. Commonly used methods for immobilizing cells include embedding and adsorption. The embedding method is a metho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12P17/10C12R1/01
CPCY02P20/50
Inventor 徐毅陈建波周音卉郑蒙蒙吴范宏朱勇刚吴小梅徐峰蒲强卞蒙璐周敏何梨梨
Owner SHANGHAI INST OF TECH