Method for extracting collagen from fish scales

A collagen and fish scale technology, applied in the field of extracting collagen from fish scales, can solve the problems of low yield and purity of fish scale collagen, achieve the effects of shortening enzymatic hydrolysis time, small molecular weight, and no secondary pollution

Active Publication Date: 2014-05-07
海口天海鱼丰实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention aims to overcome the shortcomings of low yield and purity of fish scale collagen in the above-mentioned techn...

Method used

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  • Method for extracting collagen from fish scales

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Embodiment 1

[0019] a. Use high-quality fish scales as raw materials, add 0.1mol / L NaOH to soak for 24 hours, wash with distilled water until neutral, then add 0.6mol / L HCL to soak for 24 hours, wash with distilled water until neutral, remove sticky matter and other impurities on the surface of fish scales until there is no obvious suspended impurities;

[0020] b. drying, the fish scales are naturally dried until the water content is less than 15%, after drying, impurities and unclean fish scales are removed, and stored for later use;

[0021] c. Degreasing, put the fish scales into a plastic container, soak them in water, add 10% sodium hydroxide by weight of the fish scales, stir, remove the lipid substances in the fish scales, filter and rinse, add distilled water to adjust to neutrality;

[0022] d. Deliming, adding the above-mentioned fish scales into the container, soaking in water, then adding 10% citric acid of 8% fish scale weight, stirring until no foam is generated, removing th...

Embodiment 2

[0028] a. Use high-quality fish scales as raw materials, add 0.1mol / L NaOH to soak for 24 hours, wash with distilled water until neutral, then add 0.6mol / L HCL to soak for 24 hours, wash with distilled water until neutral, remove sticky matter and other impurities on the surface of fish scales until there is no obvious suspended impurities;

[0029] b. drying, the fish scales are naturally dried until the water content is less than 15%, after drying, impurities and unclean fish scales are removed, and stored for later use;

[0030] c. Degreasing, putting the above fish scales into a plastic container, soaking in water, adding 12% sodium hydroxide by weight of the fish scales, stirring, removing the lipid substances in the fish scales, filtering and rinsing, adding distilled water to adjust to neutrality;

[0031]d. Deliming, adding the above-mentioned fish scales into the container, soaking in water, then adding 10% citric acid of 10% fish scale weight, stirring until no foam ...

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Abstract

The invention relates to a method for extracting a collagen from fish scales. The method comprises the following steps: carrying out pretreatment, drying, degreasing, deliming, irradiation treatment, enzymolysis, extraction, purification and the like on the fish scales taken as raw materials, thereby extracting the collagen from the fish scales. The enzymolysis, the extraction and the purification of the fish scales taken as the raw materials are carried out by the combination of pepsins with citric acids after the fish scales are treated under the irradiation of electron beams, so that the enzymolysis time is shortened; the hydrolysis degree is improved; the production efficiency is improved. The method has the advantages of high extraction rate, small relative molecular weight, high collagen purity, low preparation cost and secondary pollution avoidance.

Description

technical field [0001] The invention relates to a method for preparing collagen, in particular to a method for extracting collagen from fish scales. Background technique [0002] Collagen, also known as collagen, is a helical fibrous protein twisted by three peptide chains. It exists in human skin, bones, teeth, tendons and other parts, and its main physiological function is to bond connective tissue. Collagen has strong biological activity and biological function, can participate in the migration, differentiation and proliferation of cells, and makes bones, tendons, cartilage and skin have certain mechanical strength. The diversity and complexity of the structural and functional characteristics of collagen determine its important position and good application prospects in many fields. The research and application of collagen has long been a hot spot. Due to the serious pollution of the ecological environment in recent years and the outbreak of terrestrial mammal diseases,...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 曾佳
Owner 海口天海鱼丰实业有限公司
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