Method of controlling pests by combination of insect predator Carabussmaragdinus Fischer and Metarrhiziumanisopliae
A technology for preventing and controlling pests and Metarhizium anisopliae, applied in hunting equipment, applications, animal husbandry, etc., can solve the problems of low biological control efficiency, single control resources, and single control methods, so as to improve the scope, control efficiency, and production Simple process and good effect
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Embodiment 1
[0036] 1.1 Test material
[0037] Three-year-old locust gnats: collected from the East Asian migratory locust breeding base of the Plant Protection College of Shandong Agricultural University in Tai'an, Shandong. Metarhizium anisopliae spore mother powder LS20041884: Provided by Chongqing Fungal Pesticide Research and Engineering Center. Dilute the spore powder of Metarhizium anisopliae to 10 with 0.2% Tween solution 5 pc / mL, 10 6 pc / mL, 10 7 pc / mL, 10 8 pc / mL, spare. Greenbug comes from the Institute of Environmental Biology and Insect Resources, School of Plant Protection, Shandong Agricultural University. The greenbug was raised with East Asian migratory locusts, and after a year of feeding and domesticating East Asian migratory locusts.
[0038] 1.2 Determination of the toxicity of Metarhizium anisopliae to Migratory Locust
[0039] The test insects were treated with the poison soaking method, and 2uL of Metarhizium anisopliae spore liquid was sucked with a pipette, ...
Embodiment 2
[0067] The predatory green step beetle is reared and reproduced in large quantities, and adults with strong activity are selected, and the ratio of male to female is 1:1.
[0068] Obtain the fermented spore powder of Metarhizium anisopliae and process it into wettable powder: use the original drug of Metarhizium anisopliae, an inert filler and a certain amount of additives, mix and pulverize thoroughly according to the proportion, and make wettable powder into a certain powder fineness. After experimental research and biological tests, the most suitable metarhizium anisopliae spore wettable powder meets:
[0069] 1. Content, active ingredient content of Metarhizium anisopliae 10-20 billion spores / g; 2. Suspension rate 93%; 3. Wetting time: wettability 56 seconds. 4. pH value (pH value) PH=7.8. 5. Moisture: moisture content is less than 3%. Metarhizium anisopliae spore wettable powder formula is: by weight, 60% spores, 30% filler, 8% blending agent, 2 % humectant.
[0070] Su...
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