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Method for extracting protein from snakegourd seed oil-extraction by-products

A technology of by-product, Fructus Fructus, is applied in the field of high-efficiency extraction of proteins in the by-products of Fructus Fructus Fructus, can solve the problems of affecting the functional properties of proteins, high concentration of lye used for extraction, protein denaturation, etc., and achieves simplification of extraction procedures. , suitable for large-scale industrial extraction, mild conditions

Active Publication Date: 2015-07-15
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Li Chiling etc. have adopted alkali method and salt method to extract the protein in the Trichosanthes quinquefolius after petroleum ether extracting oil respectively, and the yield is very low, and the extraction rate of optimal technology is also only about 17%; The concentration of lye used for extraction is high ( 2%), excessive discharge of waste liquid will cause environmental pollution; high extraction temperature (70°C) may lead to protein denaturation and affect the functional properties of the protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The by-product raw material of Trichosanthes chinensis after oil extraction with supercritical carbon dioxide is pulverized by a pulverizer and passed through a 100-mesh sieve, and then 5 times of n-hexane is added according to the mass volume ratio and stirred at a constant speed for 1.5 hours. The by-product extracted by n-hexane is Vacuum suction filtration, the filter cake after suction filtration was naturally dried and crushed through a 100-mesh sieve; the sieved by-product powder was added to 10 times the volume of 4% NaCl solution with a pH of 8.0 according to the mass volume ratio, and homogenized at 20°C Stir and extract for 1 h, monitor the pH value with a pH meter during the period, and adjust the pH value with dilute lye to make it stable at 8.0; after extraction, use a refrigerated centrifuge at 4°C, 8000 r / min for 20 min, and take the supernatant ;Add 10 times 0.05 mol / L NaOH solution to the precipitate obtained after the first extraction, homogenize and e...

Embodiment 2

[0035]The by-product raw material of Trichosanthes chinensis after oil extraction with supercritical carbon dioxide is pulverized by a pulverizer and passed through a 200 mesh sieve, and then 10 times of normal hexane is added according to the mass volume ratio to stir at a constant speed for 1 hour, and the by-product extracted by normal hexane is used Vacuum suction filtration, the filter cake after suction filtration was naturally dried and crushed through a 100-mesh sieve; the sieved by-product powder was added to 5 times the volume of 6% NaCl solution with a pH of 8.5 according to the mass volume ratio, and homogenized at 25°C Stir and extract for 1 hour, monitor the pH value with a pH meter during the period, and adjust the pH value with dilute lye to make it stable at 8.5; after extraction, use a refrigerated centrifuge at 4°C, centrifuge at 10,000 r / min for 15 minutes, and take the supernatant ;The precipitate obtained after the first extraction was added 5 times of 0.1...

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Abstract

The invention provides a method for extracting proteins from snakegourd seed oil-extraction by-products, which is implemented by pulverizing snakegourd seed oil-extraction by-products taken as raw materials by using a pulverizer, sieving the obtained object, and respectively carrying out secondary extraction on the obtained object by using 3-5% NaCl solution with a pH value of 8.0-8.5 and 0.05-0.1mol / L of a NaOH solution so as to achieve the efficient extraction of protein in snakegourd seeds. The method comprises the main steps of taking snakegourd seed oil-extraction by-products, pulverization, degreasing, suction filtration and natural drying, smashing, sieving, first extraction, centrifugalization, secondary extraction of precipitated parts, centrifugalization, combination of liquid supernatant, dialysis and drying. According to the method, snakegourd seed oil-extraction by-products are taken raw materials, so that the effect of changing waste material into things of value is achieved, and the value added of the raw material is increased, and therefore, the method has obviously economical and environmental benefits; through the adoption of two times of extraction, protein with different properties is extracted at the utmost, and the extraction rate is as high as over 80%. According to the extraction process, the operation is simple, the protein can be obtained easily, the cost is low, and the protein is suitable for large-scale industrialized extraction.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a method for efficiently extracting protein from by-products of oil extraction from Trichosanthes quinces. technical background [0002] Gualouzi is mainly produced in Shandong, Sichuan, Anhui and other places. It has medicinal value such as moistening the lungs and reducing phlegm, smoothing the intestines and laxative. Trichosanthes seeds are rich in oils, sterols, triterpenes, glycosides and other substances, of which about 26% are fatty oils, and about 67% are unsaturated fatty acids, including citrus acid, oleic acid, linoleic acid, and linoleic acid. Therefore, many studies have focused on the supercritical CO 2 Extraction and analysis of its properties. The by-products of Trichosanthes extract oil contain about 30% of protein and inorganic elements such as calcium, iron, copper, zinc, etc., but at present most of the time the by-products are treated as waste, and the valua...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/14
Inventor 李向红俞健刘永乐王发祥王建辉赵静斌王小东
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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