Preparation method of fermented pine needle feed
A technology of pine needles and feed, applied in animal feed, animal feed, applications, etc., can solve the problems of difficult release of nutrients, inability to fully digest and absorb, and single use of pine needles, so as to reduce green gas and bitter taste and improve feed intake Quantity, effect of fermentation effect improvement
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Embodiment 1
[0026] (1) Pretreatment of pine needles. Wash the fresh pine needles with clean water and soak them in a sodium hydroxide solution with a concentration of 0.5-0.8mol / L at 50-60°C for 1-2h, and then use a concentration of 0.5-0.8mol / L 1 L of hydrochloric acid solution or clear water are washed several times, put into a steamer, and steam at a temperature of 90 to 100°C for 3 to 5 minutes to remove the waxy layer, and then use the pine needles with a concentration greater than Rinse with 90% ethanol, ventilate for 30 minutes, and then wash with sterile saline;
[0027] (2) drying and pulverizing the pretreated pine needles obtained in step (1) at 55-60° C., and passing through a 40-60 mesh sieve to obtain pulverized pine needles with uniform particles;
[0028] (3) Inoculate Aspergillus niger on potato glucose medium for cultivation, wait for the spores to grow all over the slope, and use sterile water to make a spore suspension. The spore content is 4.5~5.5х10 6 a / mL;
[002...
Embodiment 2
[0036] (1) Pretreatment of pine needles. Wash the fresh pine needles with clean water and soak them in a sodium hydroxide solution with a concentration of 0.5-0.8mol / L at 50-60°C for 1-2h, and then use a concentration of 0.5-0.8mol / L 1 L of hydrochloric acid solution or clear water are washed several times, put into a steamer, and steam at a temperature of 90 to 100°C for 3 to 5 minutes to remove the waxy layer, and then use the pine needles with a concentration greater than Rinse with 90% ethanol, ventilate for 30 minutes, and then wash with sterile saline;
[0037] (2) drying and pulverizing the pretreated pine needles obtained in step (1) at 55-60° C., and passing through a 40-60 mesh sieve to obtain pulverized pine needles with uniform particles;
[0038] (3) Inoculate Aspergillus niger on potato glucose medium for cultivation, wait for the spores to grow all over the slope, and use sterile water to make a spore suspension. Among them, the spore content is 4.5~5.5х10 6 a...
Embodiment 3
[0046] (1) Pretreatment of pine needles. Wash the fresh pine needles with clean water and soak them in a sodium hydroxide solution with a concentration of 0.5-0.8mol / L at 50-60°C for 1-2h, and then use a concentration of 0.5-0.8mol / L 1 L of hydrochloric acid solution or clear water are washed several times, put into a steamer, and steam at a temperature of 90 to 100°C for 3 to 5 minutes to remove the waxy layer, and then use the pine needles with a concentration greater than Rinse with 90% ethanol, ventilate for 30 minutes, and then wash with sterile saline;
[0047] (2) drying and pulverizing the pretreated pine needles obtained in step (1) at 55-60° C., and passing through a 40-60 mesh sieve to obtain pulverized pine needles with uniform particles;
[0048] (3) Inoculate Aspergillus niger on potato glucose medium for cultivation, wait for the spores to grow all over the slope, and use sterile water to make a spore suspension. Among them, the spore content is 4.5~5.5х10 6 a...
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