Preparation method of fermented pine needle feed

A technology of pine needles and feed, applied in animal feed, animal feed, applications, etc., can solve the problems of difficult release of nutrients, inability to fully digest and absorb, and single use of pine needles, so as to reduce green gas and bitter taste and improve feed intake Quantity, effect of fermentation effect improvement

Inactive Publication Date: 2014-05-14
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But so far, the utilization of pine needles is relatively simple, and they are all fed directly or made into pine needle powder for feeding. However, the surface of pine needles contains a waxy layer, which affects the taste and is not easy to release nutrients. The taste is bitter and the palatability is poor, so that animals do not like to eat. In addition, non-herbivorous animals lack sufficient cellulase and hemicellulase, so they cannot fully digest and absorb substances such as cellulose and hemicellulose in pine needles. make its use subject to certain restrictions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Pretreatment of pine needles. Wash the fresh pine needles with clean water and soak them in a sodium hydroxide solution with a concentration of 0.5-0.8mol / L at 50-60°C for 1-2h, and then use a concentration of 0.5-0.8mol / L 1 L of hydrochloric acid solution or clear water are washed several times, put into a steamer, and steam at a temperature of 90 to 100°C for 3 to 5 minutes to remove the waxy layer, and then use the pine needles with a concentration greater than Rinse with 90% ethanol, ventilate for 30 minutes, and then wash with sterile saline;

[0027] (2) drying and pulverizing the pretreated pine needles obtained in step (1) at 55-60° C., and passing through a 40-60 mesh sieve to obtain pulverized pine needles with uniform particles;

[0028] (3) Inoculate Aspergillus niger on potato glucose medium for cultivation, wait for the spores to grow all over the slope, and use sterile water to make a spore suspension. The spore content is 4.5~5.5х10 6 a / mL;

[002...

Embodiment 2

[0036] (1) Pretreatment of pine needles. Wash the fresh pine needles with clean water and soak them in a sodium hydroxide solution with a concentration of 0.5-0.8mol / L at 50-60°C for 1-2h, and then use a concentration of 0.5-0.8mol / L 1 L of hydrochloric acid solution or clear water are washed several times, put into a steamer, and steam at a temperature of 90 to 100°C for 3 to 5 minutes to remove the waxy layer, and then use the pine needles with a concentration greater than Rinse with 90% ethanol, ventilate for 30 minutes, and then wash with sterile saline;

[0037] (2) drying and pulverizing the pretreated pine needles obtained in step (1) at 55-60° C., and passing through a 40-60 mesh sieve to obtain pulverized pine needles with uniform particles;

[0038] (3) Inoculate Aspergillus niger on potato glucose medium for cultivation, wait for the spores to grow all over the slope, and use sterile water to make a spore suspension. Among them, the spore content is 4.5~5.5х10 6 a...

Embodiment 3

[0046] (1) Pretreatment of pine needles. Wash the fresh pine needles with clean water and soak them in a sodium hydroxide solution with a concentration of 0.5-0.8mol / L at 50-60°C for 1-2h, and then use a concentration of 0.5-0.8mol / L 1 L of hydrochloric acid solution or clear water are washed several times, put into a steamer, and steam at a temperature of 90 to 100°C for 3 to 5 minutes to remove the waxy layer, and then use the pine needles with a concentration greater than Rinse with 90% ethanol, ventilate for 30 minutes, and then wash with sterile saline;

[0047] (2) drying and pulverizing the pretreated pine needles obtained in step (1) at 55-60° C., and passing through a 40-60 mesh sieve to obtain pulverized pine needles with uniform particles;

[0048] (3) Inoculate Aspergillus niger on potato glucose medium for cultivation, wait for the spores to grow all over the slope, and use sterile water to make a spore suspension. Among them, the spore content is 4.5~5.5х10 6 a...

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PUM

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Abstract

The invention relates to a method for preparing a fermented pine needle feed with bioactivity through the fermentation of aspergillus niger. The method comprises the following steps: pretreating pine needles, crushing the pine needles, cultivating aspergillus niger, preparing a solid fermentation culture medium, inoculating the aspergillus niger, carrying out sealed fermentation on the obtained product, drying and crushing the obtained product so as to obtain a pine needle fermented feed with bioactivity. In a pine needle fermented feed prepared by using the method disclosed by the invention, the green smell and herbal medicine flavor of original pine needles are reduced or disappear, the bitter taste disappears, so that compared with the original pine needles and pine needle powder, the pine needle feed has the advantages that the palatability is greatly improved, and the intake of animals can be improved; through measuring, the contents of proteins and amino acids in the pine needle fermented feed prepared by using the method disclosed by the invention are significantly increased, and the feed also contains rich cellulase, hemicellulase and beta-glucosaccharase, so that the digestion of celluloses in pine needles is promoted, the mass intake of non-herbivores is facilitated, and the application range of pine needles as feeds is expanded.

Description

technical field [0001] The invention mainly relates to the field of fermented feed, in particular to a preparation method of fermented pine needle feed. Background technique [0002] With the rapid development of animal husbandry, the contradiction between human and animal food has become increasingly prominent. To solve this problem, the world's scientific and technological circles and industrial circles are all looking for and researching new feed resources, and pine needles are a new feed material. [0003] Pine needles are the leaves of Pinaceae and Pinus plants, also known as pine leaves, pine hairs, etc., which are bitter in taste, warm in nature, and have the effects of supplementing nutrition, strengthening the spleen and regulating qi, expelling wind and dampness, killing insects and relieving itching. Pine needles contain biologically active substances and nutrients necessary for animal growth, rich in carotene and multivitamins, and contain flavonoids, various ami...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00
Inventor 吴秋珏王玉琴刘宁李元晓王国永王占彬祁艳霞
Owner HENAN UNIV OF SCI & TECH
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