Compound insecticide of tung tree cake extract and Beauveria bassiana and preparation method thereof
The technology of Beauveria bassiana and its extract is applied in the field of compound pesticides of plant sources and microorganisms and their preparation, and can solve the problems of affecting the control effect of agricultural and forestry pests, the control effect has a great influence, the control effect is not ideal, and the like, Achieve the effect of solving the lack of biological pesticides, promoting rapid development, and maintaining long-term efficacy
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Embodiment 1
[0030] The compound insecticide in this example is prepared from the following raw materials in mass percentage: 50% of tung cake extract, Beauveria bassiana ( Beauveria bassiana ) spore powder (the concentration of conidia is 10 12 pcs / g) 15%, kaolin 20%, sodium lauryl sulfate 10%, calcium lignosulfonate 5%.
[0031] The extract powder of the tung cake extract is prepared according to the following method: (1) After the tung cake is baked at 90°C for 3 to 5 hours, it is pulverized by a pulverizer, passed through a 300-mesh sieve, and used for the sieved material The mixed extract of methanol and acetone (volume ratio: 3:1) was heated to 60-80°C for reflux extraction 4 times, the volume of the mixed extract of methanol and acetone added each time: the quality of the sieved product = 9:1, each time The extraction time is 1 hour. After combining the extracts each time, the methanol and acetone are removed by vacuum distillation with a rotary evaporator until the methanol and a...
Embodiment 2
[0050] The difference between this embodiment and Example 1 is that the distribution ratio of each raw material component is: tung cake extract extract powder 40%, Beauveria bassiana spore powder (the concentration of conidia is 10%) 12 pcs / g) 20%, kaolin 15%, sodium lauryl sulfate 15%, calcium lignosulfonate 10%.
[0051] The preparation method is the same as in Example 1.
[0052] The insecticidal efficacy of the compound insecticide of the present embodiment is as shown in Table 4-6.
[0053] (1) Toxic effect on diamondback moth
[0054] Dilute the tung cake extract compound insecticide with water according to different times (1000 times, 1500 times, 2000 times), spray it on the surface of cabbage leaves, and then raise live diamondback moths on the cabbage leaves for insecticide treatment. The effect test was repeated three times, and 3 groups of control groups were established in addition: control group 1: no spraying of the compound insecticide of Example 2; control gr...
Embodiment 3
[0069] The difference between this embodiment and Example 1 is: the distribution ratio of each raw material component is: tung cake extract extract powder 30%, Beauveria bassiana spore powder (the concentration of conidia is 10%) 10 per g) 30%, diatomaceous earth 10%, sorbitol polyoxyethylene ether 15%, dispersant Z 15%.
[0070] The preparation method is the same as in Example 1.
[0071] The insecticidal efficacy of the compound insecticide of this embodiment is shown in Table 7-9.
[0072] (1) Toxic effect on diamondback moth
[0073] After diluting the compound insecticide of Example 2 with water in different times (1000 times, 1500 times, 2000 times), spray it onto the surface of cabbage leaves, and then raise live diamondback moths on the cabbage leaves for insecticidal efficacy. The test was repeated three times, and 3 groups of control groups were established in addition: control group 1: no spraying of the compound insecticide of Example 3; control group 2: spraying...
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