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Aspergillus flavus and application thereof in preparing rutin and podophyllotoxin mono-glucoside

A monoglucoside and podophyllotoxin technology, applied to a strain of Aspergillus flavus and its application in the preparation of rutin and podophyllotoxin monoglucoside, can solve the problems of defective methods, impossibility, and high cost, and achieve The effect of protecting the diversity of the ecological environment, avoiding large-scale excavation, and shortening the production cycle

Active Publication Date: 2014-05-28
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main source of podophyllotoxin is extracted from natural podophyllum plants, and the increasing demand for medicine has led to a large number of wild podophyllophyllum plants, which has caused serious damage to resources
The source of podophyllotoxin has become a research hotspot, but various methods have defects. Large-scale tissue culture technology is not suitable for podophyllum plants. Although chemical synthesis can be carried out, the cost is too high
The construction of engineering bacteria by means of genetic engineering is also impossible due to the large number of enzymes involved in the formation of such substances.

Method used

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  • Aspergillus flavus and application thereof in preparing rutin and podophyllotoxin mono-glucoside
  • Aspergillus flavus and application thereof in preparing rutin and podophyllotoxin mono-glucoside
  • Aspergillus flavus and application thereof in preparing rutin and podophyllotoxin mono-glucoside

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, isolation and identification of Aspergillus flavus TG7

[0025] 1. Isolation of strains

[0026] The roots and rhizomes of Sinopodophyllum hexandrum (Royle) Ying collected from Xinglong Mountain (2300m above sea level) in Lanzhou City, Gansu Province in July 2009 were washed with tap water, and placed in In the ultra-clean workbench, treat with 75% (volume percent) ethanol aqueous solution for 5 minutes, then rinse with sterile water for 3-5 times; then treat with 2.5% (mass percent) sodium hypochlorite aqueous solution for 10 minutes, and then rinse with sterile water for 3 minutes. -5 times; use sterilized tweezers and blades to peel off the outer skin of the root of Taoerqi, then cut into small pieces of 0.5cm×0.5cm in size and plant them on PDA solid medium, and cultivate them at 28°C for 3-7 days; at the same time The roots of the same sterilized P. chinensis were not peeled and cut, rolled on the PDA medium for one week, and cultivated under the sam...

Embodiment 2

[0033] Embodiment 2, Utilize Aspergillus flavus TG7 to prepare rutin and podophyllotoxin monoglucoside

[0034] 1. Cultivation of Aspergillus flavus TG7

[0035] The composition of the potato glucose liquid medium is: 200 g of peeled potatoes, 20 g of glucose, and add water to make the volume to 1000 mL. The preparation method of potato dextrose liquid medium: peel the potatoes and cut them into about 2cm 2 put into a beaker and boil for 30 minutes, then filter with double-layer gauze, take the filtrate and add glucose, then make up water, the pH is natural. Put 100ml of potato dextrose liquid medium in a 250ml Erlenmeyer flask, and sterilize it for later use.

[0036] A single colony of Aspergillus flavus TG7 was inoculated into 100ml potato dextrose liquid medium, and cultured with shaking at 28°C and 120r / min for 7 days.

[0037] 2. Preparation of crude extract

[0038] 1. Take the fermentation system obtained in step 1, use a suction filter to separate and collect the ...

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Abstract

The invention discloses an aspergillus flavus and the application thereof in preparing rutin and podophyllotoxin mono-glucoside. The preservation number of the aspergillus flavus (aspergillus flavus) TG7 is CGMCC NO.8570. The invention also protects the application of aspergillus flavus TG7 in preparing the rutin and / or the podophyllotoxin mono-glucoside. The invention also protects a method in preparing the rutin and / or the podophyllotoxin mono-glucoside. The method comprises the following steps of fermenting and culturing the aspergillus flavus TG7 to obtain the rutin and / or the podophyllotoxin mono-glucoside. The endogenous aspergillus flavus provided by the invention can prepare the rutin and the podophyllotoxin mono-glucoside via fermentation, so that the operation is simple, costs are low, production cycles are short, and the aspergillus flavus has application potential for industrialized large-scale production. The invention provides a new way for directly obtaining podophyllotoxin mono-glucoside, avoids a great deal of mining for wild podophyllum plants, and has an important significance in protecting the diversity of ecological environment.

Description

technical field [0001] The invention relates to a strain of Aspergillus flavus and its application in preparing rutin and podophyllotoxin monoglucoside. Background technique [0002] Rutin has a wide range of utilization value, which is mainly reflected in its use as a therapeutic drug and health care product. Rutin has vitamin P-like effects, can reduce capillary permeability and fragility, promote cell proliferation and prevent blood cell aggregation, and have anti-inflammatory, anti-allergic, diuretic, antispasmodic, antitussive, and blood lipid-lowering effects. The pharmaceutical industry at home and abroad uses rutin as a raw material to develop a variety of drugs, such as rutin tablets, venalutong (hydroxyethyl rutin), rutin magnesium chromium salt, multidimensional glucose hydroxybuterutin, quercetin, etc. cardiovascular drugs. In addition, rutin acts as an antioxidant and natural food coloring. At present, my country's rutin production mainly uses Huai Mi as the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/60C12R1/67
Inventor 赵长琦梁子臻张嘉朱妍妍
Owner BEIJING NORMAL UNIVERSITY
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