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A kind of preparation method of honeysuckle leaf effective extract

A technology of honeysuckle leaves and extracts, which is applied in the field of preparation of effective extracts of honeysuckle leaves, can solve the problems of numerous operation steps and difficult realization, and achieves the effects of high drug safety, low preparation cost and simple operation.

Active Publication Date: 2017-08-25
LUNAN PHARMA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the same eluted components need to be continuously detected by TLC, and the operation steps are numerous, and they are eluted and collected with different concentrations of ethanol respectively, which is difficult to achieve in large-scale production, and what is obtained is a separate group of components, not honeysuckle. Leaf extract

Method used

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  • A kind of preparation method of honeysuckle leaf effective extract
  • A kind of preparation method of honeysuckle leaf effective extract
  • A kind of preparation method of honeysuckle leaf effective extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 honeysuckle leaves purification process is preferred

[0030] 1 Macroporous resin type selection test design

[0031] Phenolic acids in honeysuckle leaves have certain polarity and water solubility, and polyphenolic hydroxyl groups are easy to form hydrogen bonds with macroporous resins. Therefore, the following six resins with good enrichment effects on phenolic hydroxyl groups are selected as the research targets in this process. , to filter.

[0032] According to the properties of phenolic acids and the adsorption performance of the resin, six types of macroporous resins, AB-8, HPD400, HPD100, HPD826, LS-300B, and LS-303, were selected for optimization, and their main properties are shown in Table 1.

[0033] Table 1 Properties of various types of macroporous resins

[0034]

[0035] 2 Pretreatment of macroporous resin

[0036] Soak various types of macroporous resins: AB-8, HPD400, HPD100, HPD826, LS-300B, LS-303 in 95% ethanol solution for 24 hou...

Embodiment 2

[0173] The preparation of the effective extract of the leaf of honeysuckle of embodiment 2

[0174] 1) Take 750g of dried honeysuckle leaves, extract 3 times with 30% ethanol under reflux, add 12 times the amount for the first time, add 10 times the amount for the second time, add 8 times the amount for the third time, 1.5 hours each time, filter, Combine the filtrates, concentrate under reduced pressure to a concentrated solution with a crude drug amount of 0.3 g / mL, let cool, and set aside;

[0175] 2) Adjust the pH value of the concentrated solution to 1.0, filter until clear, and set aside;

[0176] 3) Add the filtrate to the pretreated AB-8 macroporous resin column with a diameter-to-height ratio of 1:5 at a rate of 3BV / h, adjust the pure water to pH=6.0 with hydrochloric acid, and use a flow rate of 3BV / h Wash until the eluent is clear, then elute with 7 times the bed volume of 60% ethanol at a flow rate of 2BV / h, collect the eluent, and concentrate under reduced pressu...

Embodiment 3

[0183] The preparation of embodiment 3 honeysuckle leaves effective extract

[0184] 1) Take 750g of dried honeysuckle leaves, extract 3 times with 90% ethanol under reflux, add 12 times the amount for the first time, add 10 times the amount for the second time, add 8 times the amount for the third time, 1.0 hours each time, filter, Combine the filtrates, concentrate under reduced pressure to a concentrated solution with a crude drug amount of 0.3 g / mL, let cool, and set aside;

[0185] 2) Adjust the pH value of the concentrated solution to 6.0, filter until clear, and set aside;

[0186] 3) Add the filtrate to the pretreated XAD-8 macroporous resin column with a diameter-to-height ratio of 1:5 at a rate of 3BV / h, adjust the pure water to pH=1.0 with hydrochloric acid, and use a flow rate of 3BV / h Wash until the eluent is clear, then elute with 7 times the bed volume of 60% ethanol at a flow rate of 2BV / h, collect the eluent, and concentrate under reduced pressure to a clear ...

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Abstract

The invention relates to a preparation method of a honey suckle leaf effective extract product; the preparation method comprises the steps of alcohol backflow extraction, acid adjusting filtering, macroporous resin wash out impurities removal and condensation, and drying; the method is simple in operation, the macroporous resin can be renewable; making cost is low and environmental pollution is small; ethanol solution is used as extraction purifying solvent, is high in safety and low in cost, and is easily available; the obtained honey suckle leaf effective extract product is high in chlorogenic acid and general flavone content, complete in component, not only has the chlorogenic acid and the general flavones, but also has iridoid glycosides and phenolic acids, is very close to natural proportion of each component of the honey suckle leaf.

Description

technical field [0001] The invention relates to a preparation method of an effective extract of honeysuckle leaves, belonging to the field of traditional Chinese medicines. Background technique [0002] Lonicera japonica leaves are the dried leaves of Lonicera japonica Thunb. Li Shizhen said: "The stems, leaves and flowers of Lonicera japonica have the same functions". The medicinal effect and ingredients are basically the same as those of honeysuckle. Choleretic effect, commonly used in the treatment of upper respiratory tract infection, sore throat, sore throat, tonsillitis, stomatitis, etc. The main active ingredients of honeysuckle leaves are chlorogenic acid, flavonoids, glycosides (saponins, iridoid glycosides), volatile oil and other trace components, etc., which have various functions such as anti-pathogenic microorganisms, lowering blood pressure, lowering blood fat, and anti-tumor . [0003] Honeysuckle is often in short supply due to its limited production. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/355A61K127/00
Inventor 赵志全苏瑞强李守信李蔚群
Owner LUNAN PHARMA GROUP CORPORATION
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