Antioxidant and nutritional stress-related proteins and their coding genes and applications

Abstract

The invention discloses an antioxidation and nutrient stress resistance associated protein and a coding gene and application thereof. The protein provided by the invention is selected from (a) or (b) as below: (a) a protein having an amino acid sequence shown as sequence 2 in the sequence table; and (b) a protein obtained by substitution and / or deletion and / or addition of one or several amino acid residues on the amino acid sequence shown as sequence 2 in the sequence table, related to antioxidation and nutrient stress resistance, and derived from (a). Experiments show that when the protein provided by the invention is over-expressed in a wild-type chlamydomonas, a transgenic strain gains enhanced oxidation resistance to hydrogen peroxide treatment; and when a vector interfering the expression of the protein provided by the invention is transferred to the wild-type chlamydomonas, an obtained mutant strain with reduced HOS expression quantity has decreased oxidation resistance to hydrogen peroxide treatment. Furthermore, the mutant strain with overexpression of HOS has greatly increased hydrogen desorption amount than the wild-type mutant in the sulfur deficiency hydrogen production system.

Description

technical field [0001] The invention relates to an anti-oxidation and nutritional stress-related protein derived from algae, its coding gene and application. Background technique [0002] Oxidative stress and nutrient deficiency (such as sulfur deficiency) are two major adversities frequently encountered by photosynthetic organisms, which severely limit the photosynthetic productivity of photosynthetic organisms including algae. Studies have shown that the main reason for the damage caused by these adversities may be the increase in the concentration of reactive oxygen species including hydrogen peroxide in cells, which causes changes in the structure of biological macromolecules such as lipids, proteins, and nucleic acids, disrupts the redox balance of cells, and leads to photosynthetic productivity. decline and even cell death. Therefore, the discovery of important new genes for antioxidant and nutritional deficiencies can provide available genetic elements and technical ...

AI Technical Summary

Problems solved by technology

[0002] Oxidative stress and nutrient deficiency (such as sulfur deficiency) are two major adversities frequently encountered by photosynthetic organisms, which severely limit the photosynthetic productivity of photosynthetic organisms including algae

Studies have shown that the main reason for the damage caused by these adversities may be the increase in the concentration of reactive oxygen species including hydrogen peroxide in cells, which causes changes in the structure of biological macromolecules such as lipids, proteins, and nucleic acids, disrupts the redox balance of cells, and leads to photosynthetic productivity. cell death

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