Production method of single-cell protein

A single-cell protein and production method technology, applied in the direction of single-cell algae, fungi, etc., can solve the problems of high production cost of single-cell protein, high quality of single-cell protein, low production cost, etc., to solve food shortage, enzymatic hydrolysis, etc. High efficiency and the effect of improving production efficiency

Active Publication Date: 2014-06-11
河南艾尔恩生物技术有限公司
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems that the residue after oil extraction by microorganisms in the prior art biodiesel production system is not fully utilized, the production cost of single-cell protein is relatively high, and the quality needs to be improved, the purpose of the present invention is to provide a method for producing single-cell protein by using the residue of microorganisms after oil extraction. The protein method can increase the protein content of cells through temperature regulation. This method has high yield, low production cost, high quality single-cell protein, and can be regenerated.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The oil-rich Chlorella algae were inoculated into sterile f / 2 medium through aseptic operation, and placed in a light incubator for cultivation at a cultivation temperature of 25°C and a light intensity of 2000lx for 10 days. Inoculate the strains of Rhodotorula viscosus rich in oil into the sterilized shake flask culture solution through aseptic operation: 1000mL of potato extract (200g potatoes are peeled, cut into small pieces, add water and boil for 1 hour, filter with 5 layers of gauze, and dilute to 1000mL ) and glucose 20,000 mg / L, cultured in a shaker with a rotation speed of 150 rpm and a culture temperature of 37°C for 72 hours. 50g of oil-rich Chlorella grown in a light incubator and 50g of oil-rich Rhodotorula viscose fermented in a shaker were collected by centrifugation (8000 rpm, 10 minutes) to obtain biomass, and washed 5 times with distilled water . Pretreatment with lysozyme L6876: oil-rich microorganisms (dry matter) and lysozyme L6876 were mixed at ...

Embodiment 2

[0026]The oil-rich Nannochloropsis species were inoculated into sterile f / 2 medium through aseptic operation, and placed in a light incubator for cultivation at a cultivation temperature of 25°C and a light intensity of 2000 lx for 10 days. Inoculate the strains of Rhodotorula viscosus rich in oil into the sterilized shake flask culture solution through aseptic operation: 1000mL of potato extract (200g potatoes are peeled, cut into small pieces, add water and boil for 1 hour, filter with 5 layers of gauze, and dilute to 1000mL ) and glucose 20,000 mg / L, cultured in a shaker with a rotation speed of 150 rpm and a culture temperature of 37°C for 72 hours. Respectively, 50 g of oil-rich Nannochloropsis grown in a light incubator and 50 g of oil-rich Rhodotorula viscosum fermented in a shaker were collected by centrifugation (8000 rpm, 10 minutes) to obtain biomass, and washed with distilled water for 5 Second-rate. Pretreatment with lysozyme L7651: oil-rich microorganisms (dry m...

Embodiment 3

[0030] The oil-rich Chlorella algae were inoculated into sterile f / 2 medium through aseptic operation, and placed in a light incubator for cultivation at a cultivation temperature of 25°C and a light intensity of 2000lx for 10 days. Inoculate the strains of Rhodotorula viscosus rich in oil into the sterilized shake flask culture solution through aseptic operation: 1000mL of potato extract (200g potatoes are peeled, cut into small pieces, add water and boil for 1 hour, filter with 5 layers of gauze, and dilute to 1000mL ) and glucose 20,000 mg / L, cultured in a shaker with a rotation speed of 150 rpm and a culture temperature of 37°C for 72 hours. 50g of oil-rich Chlorella grown in a light incubator and 50g of oil-rich Rhodotorula viscose fermented in a shaker were collected by centrifugation (8000 rpm, 10 minutes) to obtain biomass, and washed 5 times with distilled water . Pretreatment with lysozyme L2879: oil-rich microorganisms (dry matter) and lysozyme L2879 were mixed at ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a production method of a single-cell protein. The production method comprises the steps of (1) evenly mixing the oil extraction residues of microorganisms with lysozyme in a mass ratio of (20-200): 1, reacting for 2 to 5 hours under conditions of 40 to 60 DEG C, an initial pH 5.0 to 7.0 and a stirring rotating speed 50 to 200 r/minute, and treating at 105 DEG C for 10 to 30 minutes; (2) mixing water with the obtained enzymatic hydrolysate in a mass ratio of (5-2): 1, and adding 5-150mg/L of magnesium sulfate, 100-200mg/L of ferric chloride and 5-50mg/L of odium dihydrogen phosphate, and sterilizing at 115 to 121 DEG C for 15 to 30 minutes; (3) inoculating the culture solution obtained in the step (2) with spiral seaweed and a yeast under the conditions of a pH ranging 6.0 to 8.0 and a volume of aeration in the range of 0.1vvm to 5.0vvm, and employing a two-stage culture method based on a temperature control strategy, and setting the culture time of the front stage of culture in the range of 48 to 108 hours and the culture time of the rear stage of culture in the range of 24 to 36 hours, and (4) collecting the microorganic biomass obtained in the step (3), flushing with water, and drying by using a freezer dryer to obtain the single-cell protein product. The production method has the advantages that the production efficiency is improved, the production cost is reduced and the quality of the single-cell protein is improved.

Description

technical field [0001] The invention relates to the technical field of chemical industry. Background technique [0002] Microbial oils are oils synthesized by microorganisms such as yeast, mold, bacteria and algae under certain conditions using carbohydrates and other substances through a series of biochemical reactions. Microbial oil is a good raw material for biodiesel production. Microbial oil extraction is one of the key steps in biodiesel production. The residues after microbial oil extraction mainly contain carbohydrates, proteins and other substances. Therefore, in the production system of high-quality biodiesel produced by microorganisms, if the above residues after oil extraction are not used, it will result in waste of high-value and multi-component components of microbial residues after oil extraction. environmental pollution. In fact, the residue after microbial oil extraction is an important biomass resource. The residue after microbial oil extraction can be...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/12
Inventor 郑洪立马小琛阮榕生刘玉环高振巫小丹万益琴黄和王允圃
Owner 河南艾尔恩生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap