Production method of single-cell protein

Abstract

The invention provides a production method of a single-cell protein. The production method comprises the steps of (1) evenly mixing the oil extraction residues of microorganisms with lysozyme in a mass ratio of (20-200): 1, reacting for 2 to 5 hours under conditions of 40 to 60 DEG C, an initial pH 5.0 to 7.0 and a stirring rotating speed 50 to 200 r / minute, and treating at 105 DEG C for 10 to 30 minutes; (2) mixing water with the obtained enzymatic hydrolysate in a mass ratio of (5-2): 1, and adding 5-150mg / L of magnesium sulfate, 100-200mg / L of ferric chloride and 5-50mg / L of odium dihydrogen phosphate, and sterilizing at 115 to 121 DEG C for 15 to 30 minutes; (3) inoculating the culture solution obtained in the step (2) with spiral seaweed and a yeast under the conditions of a pH ranging 6.0 to 8.0 and a volume of aeration in the range of 0.1vvm to 5.0vvm, and employing a two-stage culture method based on a temperature control strategy, and setting the culture time of the front stage of culture in the range of 48 to 108 hours and the culture time of the rear stage of culture in the range of 24 to 36 hours, and (4) collecting the microorganic biomass obtained in the step (3), flushing with water, and drying by using a freezer dryer to obtain the single-cell protein product. The production method has the advantages that the production efficiency is improved, the production cost is reduced and the quality of the single-cell protein is improved.

Description

technical field [0001] The invention relates to the technical field of chemical industry. Background technique [0002] Microbial oils are oils synthesized by microorganisms such as yeast, mold, bacteria and algae under certain conditions using carbohydrates and other substances through a series of biochemical reactions. Microbial oil is a good raw material for biodiesel production. Microbial oil extraction is one of the key steps in biodiesel production. The residues after microbial oil extraction mainly contain carbohydrates, proteins and other substances. Therefore, in the production system of high-quality biodiesel produced by microorganisms, if the above residues after oil extraction are not used, it will result in waste of high-value and multi-component components of microbial residues after oil extraction. environmental pollution. In fact, the residue after microbial oil extraction is an important biomass resource. The residue after microbial oil extraction can be...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems that the residue after oil extraction by microorganisms in the prior art biodiesel production system is not fully utilized, the production cost of single-cell protein is relatively high, and the quality needs to be improved, the purpose of the present invention is to provide a method for producing single-cell protein by using the residue of microorganisms after oil extraction. The protein method can increase the protein content of cells through temperature regulation. This method has high yield, low production cost, high quality single-cell protein, and can be regenerated.