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Method for screening exogenous carbohydrase spectrum of chicken feed

A technology of carbohydrate enzymes and screening methods, which is applied in the field of screening of exogenous carbohydrate enzymes in chicken feed, can solve the problems of many factors affecting systematic errors and accidental errors, laborious, time-consuming, etc., and achieve rapid carbohydrate enzymes, optimization The Effects of Carbohydrate Enzymes

Active Publication Date: 2014-06-25
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

The in vivo method is to conduct animal experiments, which is often costly, laborious, and time-consuming, and there are many factors affecting systematic errors and accidental errors (Chen Liang et al., 2013)
The in vitro method has the advantages of rapidity, simplicity, and low cost, but the parameters and conditions selected by the in vitro method often lack theoretical explanations and are somewhat arbitrary (Coles et al., 2005)

Method used

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  • Method for screening exogenous carbohydrase spectrum of chicken feed
  • Method for screening exogenous carbohydrase spectrum of chicken feed

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Effect test

Embodiment 1

[0023] Such as figure 1 Shown, concrete operation method of the present invention can be implemented in the following manner:

[0024] (1) Choose a corn-soybean meal ration, and add four exogenous carbohydrate enzymes to the feed, namely xylanase, cellulase, β-glucanase and pectinase, each exogenous carbohydrate The concentration gradient levels of compound enzyme additives were set to 0, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000 μg / g, respectively.

[0025] (2) Accurately weigh 1-2g (crushed and passed through a 60-mesh sieve) feeding sample (accurate to 0.0002g), put it in a 100ml corked Erlenmeyer flask, add buffer and digestive enzyme solution for simulating gastric digestion into the Erlenmeyer flask, Among them, the weight percentage of feed, simulated gastric buffer solution and simulated gastric digestive enzyme solution is 2:8:1; the buffer solution for simulated gastric digestion has a pH of 2.0, and the molar mass ratio is 90:8=sodium chloride:potassium chl...

Embodiment 2

[0031] (1) Select a wheat-soybean meal ration, and add five exogenous carbohydrate enzymes to the feed, namely xylanase, cellulase, β-glucanase, β-mannanase and pectin For enzymes, the concentration gradient levels of each exogenous carbohydrate enzyme additive were set to 0, 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 μg / g, respectively.

[0032](2) Accurately weigh 1-2g (crushed and passed through a 60-mesh sieve) feeding sample (accurate to 0.0002g), put it in a 100ml corked Erlenmeyer flask, add buffer and digestive enzyme solution for simulating gastric digestion into the Erlenmeyer flask, Among them, the weight percentage of feed, simulated gastric buffer solution and simulated gastric digestive enzyme solution is 2:8:1; the buffer solution for simulated gastric digestion has a pH of 2.0, and the molar mass ratio is 90:8=sodium chloride:potassium chloride mixture Buffer solution; the simulated gastric digestive enzyme solution is 1000U / mL pepsin solution with p...

Embodiment 3

[0038] (1) Select a wheat-dough ration, and add six exogenous carbohydrate enzymes to the feed, namely xylanase, cellulase, β-glucanase, β-mannanase, arabic For glycanase and pectinase, the concentration gradient levels of each exogenous carbohydrate enzyme additive were set to 0, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000 μg / g, respectively.

[0039] (2) Accurately weigh 1-2g (crushed and passed through a 60-mesh sieve) feeding sample (accurate to 0.0002g), put it in a 100ml corked Erlenmeyer flask, add buffer and digestive enzyme solution for simulating gastric digestion into the Erlenmeyer flask, Among them, the weight percentage of feed, simulated gastric buffer solution and simulated gastric digestive enzyme solution is 2:8:1; the buffer solution for simulated gastric digestion has a pH of 2.0, and the molar mass ratio is 90:8=sodium chloride:potassium chloride mixture Buffer solution; the simulated gastric digestive enzyme solution is 1000U / mL pepsin solution with...

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Abstract

The invention provides a method for screening an exogenous carbohydrase spectrum of chicken feed. The method comprises the following steps: selecting a target chicken feed; setting the type and the dose level of the added exogenous carbohydrases; in-vitro simulating gastrointestinal digestion of chicken; detecting the in-vitro digestion emergy of the feed; and analyzing the optimal carbohydrase spectrum of the target feed. According to the method, the animal digesting process is simulated in vitro, and the acting effect of the carbohydrases is represented by using the in-vitro emergy, so that the optimal exogenous carbohydrase spectrum of the chicken feed can be quickly obtained.

Description

technical field [0001] The invention relates to a screening method for exogenous carbohydrate zymogram of chicken feed, in particular to a method for rapidly optimizing chicken feed exogenous carbohydrate zymogram by using an in vitro simulated digestion method. Background technique [0002] Carbohydrates are the bulk of feed and the main source of energy for animals. As the cost of feed continues to rise, people are constantly looking for cheaper feed ingredients (such as: corn processing by-products) to partially replace traditional corn and soybean meal feed ingredients. However, these processing by-products are often high in dietary fiber, which is not fully utilized by the animal organism (Anguita et al., 2006). At present, exogenous carbohydrate enzymes are usually used to improve the utilization rate of dietary fiber, and the carbohydrate enzymes are often non-starch polysaccharide enzymes, such as xylanase (Engberg et al., 2006; Ding Xuemei et al., 2009; Selle et a...

Claims

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Application Information

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IPC IPC(8): C12Q1/34
Inventor 陈亮张宏福侯小峰付生慧何科林
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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