Preparation method of microbial fermentation cotton seed meal

Abstract

The invention provides a preparation method of microbial fermentation cotton seed meal. The microbial fermentation cotton seed meal comprises the following raw materials: 800-1200 kg of cotton seed meal, 80-120 kg of corn flour, 80-120 kg of bran, 3-7 kg of bacillus, 3-7 kg of lactic acid bacteria and 3-7 kg of dry yeast. The preparation method comprises the following steps: uniformly mixing diluted zymophyte liquid with a mixture of the dry yeast, the cotton seed meal, the corn flour and the bran in a mixer at a ratio of 1:3, fermenting at temperature of 25-30 DEG C for 72-120 hours to obtain the microbial fermentation cotton seed meal with a color changing from faint yellow to dark brown, wherein the bacillus and the lactic acid bacteria are added into a fermentation container filled with 30-50 kg of pure water and cultured for 2-3 days in a closed state to obtain a fermentation liquor, and the fermentation liquor is used after being diluted with 250-350 kg of pure water.

Description

technical field [0001] The invention relates to the fermentation technology of microorganisms. The adopted method can remove the anti-nutritional factors and free gossypol in the cottonseed meal, and can be used as feed to improve the intestinal flora relationship of livestock and poultry, increase the protein content, and improve the resistance of livestock and poultry bodies. Background technique [0002] Xinjiang is located in the west of China, where the planting of corn, soybeans and other crops is relatively small, and it is an area seriously lacking in plant-based protein feed. With the rapid development of animal husbandry, the prices of various feed materials have also soared, especially animal protein feed (such as fishmeal) has the largest price increase, which forces livestock producers to choose cottonseed meal with relatively low price. Cottonseed meal is a plant-based protein feed, and its protein content accounts for about 40-45%. It can be compared with fishm...

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Problems solved by technology

[0002] Xinjiang is located in the west of China, where the planting of corn, soybeans and other crops is relatively small, and it is an area seriously lacking in plant-based protein feed. With the rapid development of animal husbandry, the prices of various feed materials have also soared, especially animal protein feed (such as fishmeal) has the largest price increase, which forces livestock producers to choose cottonseed meal with relatively low price. Cottonseed meal is a plant-based protein feed, and its protein content accounts for about 40-45%. It can be compared with fishmeal and soybean meal. In addition, after hydrolysis of cottonseed meal, 17 kinds of amino acids can be obtained, which is a high-quality and cheap protein source in animal husbandry production, but because it contains various anti-nutritional factors, and its amino acid content is not as balanced as that of fish meal and other animal proteins, it lacks Various beneficial bacteria, thus affecting their feeding amount, so that their proportion in protein feed raw materials is not very high

Xinjiang is an important commercial cotton production base in the country. At present, it has basically formed an annual production capacity of 3 million tons. Cottonseed meal feed resources are quite rich. However, due to the existence of these anti-nutritional factors, namely free gossypol, phytic acid and mono Ning et al. greatly restricted the application of cottonseed meal in livestock and poultry feed and aquatic feed

[0003] According to the literature, the anti-nutritional factors of cottonseed meal have been disclosed in the prior art. Microbial fermentation is also used to remove free gossypol in cottonseed meal, but the disadvantages are as follows: there is a method to sieve the cottonseed meal, and then ferment; there is a method Adding nutrients to cottonseed meal can make the fermentation more complete; there are also methods to carry out airtight under aerobic conditions, which make the fermentation process of cottonseed meal more complicated, which is not conducive to cost saving and industrial production