PCR detection-oriented linear microfluidic DNA extraction system

An extraction system and microfluidic technology, applied in the fields of biology, analytical chemistry and medical testing, can solve the problems of difficult automatic operation, complicated steps, time-consuming and other problems, so as to improve the experimental work efficiency, avoid the mixing of reagents, and smooth the reaction. quick effect

Inactive Publication Date: 2014-07-16
BEIJING UNIV OF TECH
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Problems solved by technology

Traditional nucleic acid separation technology includes precipitation, centrifugation and other processes. These purification methods have complicated steps, time-consuming, low yield, and contact with toxic reagents, so it is difficult to realize automatic

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  • PCR detection-oriented linear microfluidic DNA extraction system
  • PCR detection-oriented linear microfluidic DNA extraction system

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Embodiment Construction

[0016] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0017] like Figure 1-2 , a linear microfluidic DNA extraction system for PCR detection, the system includes an anti-corrosion alloy material structure 1, an injection piston 2, a stepper motor 3, a waste liquid microchannel 4, and a lead screw 5, the lead screw 5 A slider is installed on it, and the slider is the reaction chamber, and the biological nano magnetic beads 6 are placed in the center of the slider; specifically, the anti-corrosion alloy material structure 1 contains eight microchannels with injection pistons 2, The eight microchannels with injection pistons 2 are arranged in the length direction of the anti-corrosion alloy material structure 1, and the eight microchannels with injection pistons 2 form an alternate module of the entire reagent; the stepping motor 3 and the lead screw 5 are installed on Inside the anti-corrosion al...

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Abstract

The invention relates to a PCR detection-oriented linear microfluidic DNA extraction system. The system comprises a corrosion-resistant alloy material structure, an injection piston, a stepper motor, a liquid waste microchannel and a screw, wherein a slide block is arranged on the screw; the slide block is a reaction cavity; biological nanometer magnetic beads are arranged at the center of the slide block. The linear reaction structure is adopted, mixing of reagents caused by repeated work is avoided, the final purity and the concentration of DNA are improved, the reaction is smoother and faster, and one-time bulking of the reagent is facilitated. A single chip microprocessor integrated control system can be adopted, excessive peripheral equipment is reduced, the system is more miniaturized, and the function is integrated, so that the stepper motor and the injection piston finish power work of the system in common, and the experimental work efficiency is improved.

Description

technical field [0001] The invention relates to a linear microfluidic DNA extraction system oriented to PCR detection, which belongs to the fields of biology, analytical chemistry and medical detection. Background technique [0002] Nucleic acid research is an important topic in modern biology and medical research. As the carrier of genetic information, nucleic acid is the material basis of gene expression. In addition to playing a very important role in the normal growth, development, and reproduction of organisms, it is also related to abnormal conditions of life, such as tumor occurrence, radiation, etc. Injuries are also closely related. Therefore, nucleic acid is an important topic in modern biology and medical research. The traditional nucleic acid separation technology includes precipitation, centrifugation and other processes. These purification methods have complicated steps, time-consuming, low yield, and contact with toxic reagents, so it is difficult to realize...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCC12N15/1013
Inventor 吴坚于伦王富吉陈涛
Owner BEIJING UNIV OF TECH
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