Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hansenula polymorpha recombinant strain and application thereof in biosynthesis of paclitaxel

A technology of Hansenula polymorpha and recombinant strains, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems that paclitaxel has not been reported, and achieve the effect of easy high-density fermentation and easy separation and extraction

Active Publication Date: 2014-07-16
SHAANXI UNIV OF SCI & TECH
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are no foreign reports on the use of low-energy ion implantation to mediate medicinal plant genomic DNA transformation yeast technology to construct recombinant bacteria producing medicinal active ingredients, and there have been no reports on the biosynthesis of paclitaxel in yeast at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hansenula polymorpha recombinant strain and application thereof in biosynthesis of paclitaxel
  • Hansenula polymorpha recombinant strain and application thereof in biosynthesis of paclitaxel
  • Hansenula polymorpha recombinant strain and application thereof in biosynthesis of paclitaxel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0027] The present invention uses H.polymorpha DL-1 as the starting strain (the source is ATCC No.26012), utilizes low-energy ion irradiation injection to mediate the transformation of Taxus chinensis genomic DNA, and obtains paclitaxel-producing recombinant strains by screening, and genetically Stability and corresponding fermentation tests.

[0028] (1) culture medium

[0029] The composition of liquid inorganic medium: glycerol 19g, (NH 4 ) 2 SO 4 6g,K 2 HPO 4 0.24g, MgSO 4 ·7H 2 O1.4g, CaCl 2 0.38g, NaCl0.6g, NaNO 3 0.58g, 0.2mL iron salt solution, 1mL thiamine hydrochloride solution, 1mL PTM trace element solution, dilute to 1L with water, pH 6.5;

[0030] Wherein each solution batching is as follows:

[0031] Iron salt solution: FeCl 3 ·6H 2 O30g, dissolved in water and dilute to 1L;

[0032] Thiamine hydrochloride solution: thiami...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a hansenula polymorpha recombinant strain and an application thereof in biosynthesis of paclitaxel. A total DNA transformation yeast of mediated taxus chinensis genome is injected to obtain a hansenula polymorpha recombinant strain CGMCC No.8999 for producing paclitaxel by using low-energy ions. The technical breakthrough for biological fermentation to produce paclitaxel by using the recombined yeast is achieved, and a new way is provided for solving paclitaxel source shortage, protecting taxus chinensis plant resources in China and sustainable development thereof.

Description

technical field [0001] The invention belongs to the field of transgenic yeast recombinant bacteria producing paclitaxel obtained through genetic transformation, and specifically relates to a Hansenula polymorpha recombinant strain obtained by genetic transformation of the total genome DNA of Taxus chinensis in yeast by using low-energy ion implantation and its biosynthesis of paclitaxel in the application. Background technique [0002] Paclitaxel (trade name Taxo1) is a natural anticancer drug with broad-spectrum and definite efficacy recognized in the world today. Its molecular structure is derived from baccatin III and phenylalanine linked to the 13th carbon. tricyclic diterpenoid alkaloids. Paclitaxel is the only drug known to promote microtubule polymerization and stabilize the polymerized microtubules. After the cells are exposed to paclitaxel, a large number of microtubules accumulate in the cells, and the accumulation of these microtubules interferes with various cel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/19C12P17/02C12R1/78
Inventor 钱卫东赵德志蔡长龙毛培宏王婷陈雪峰施春阳
Owner SHAANXI UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products