Hansenula polymorpha recombinant strain and application thereof in biosynthesis of paclitaxel
A technology of Hansenula polymorpha and recombinant strains, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems that paclitaxel has not been reported, and achieve the effect of easy high-density fermentation and easy separation and extraction
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[0026] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.
[0027] The present invention uses H.polymorpha DL-1 as the starting strain (the source is ATCC No.26012), utilizes low-energy ion irradiation injection to mediate the transformation of Taxus chinensis genomic DNA, and obtains paclitaxel-producing recombinant strains by screening, and genetically Stability and corresponding fermentation tests.
[0028] (1) culture medium
[0029] The composition of liquid inorganic medium: glycerol 19g, (NH 4 ) 2 SO 4 6g,K 2 HPO 4 0.24g, MgSO 4 ·7H 2 O1.4g, CaCl 2 0.38g, NaCl0.6g, NaNO 3 0.58g, 0.2mL iron salt solution, 1mL thiamine hydrochloride solution, 1mL PTM trace element solution, dilute to 1L with water, pH 6.5;
[0030] Wherein each solution batching is as follows:
[0031] Iron salt solution: FeCl 3 ·6H 2 O30g, dissolved in water and dilute to 1L;
[0032] Thiamine hydrochloride solution: thiami...
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