Preparation method for immobilized microbe oil-spill repairing agent

A technology of immobilized microorganisms and remediation agents, which is applied in the field of microbial remediation of petroleum and petroleum products polluted oceans, and in the field of preparation of immobilized microbial oil spill remediation agents, to achieve the effects of wide sources of raw materials, improved mechanical strength, and improved mass transfer

Inactive Publication Date: 2014-07-16
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Addition of corncob-based activated carbon to sodium alginate-CaCl 2 In the embedding method, to improve the mechanical strength of the preparation, there is no report at home and abroad

Method used

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  • Preparation method for immobilized microbe oil-spill repairing agent
  • Preparation method for immobilized microbe oil-spill repairing agent
  • Preparation method for immobilized microbe oil-spill repairing agent

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Embodiment Construction

[0030] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0031] Implementation example 1

[0032] A preparation method of an immobilized microbial oil spill restoration agent using sodium alginate-calcium chloride-corncob-based activated carbon as an embedding immobilization material, the specific steps are:

[0033] 1. Preparation of corncob-based activated carbon

[0034] 1) Clean, sieve, dry and pulverize the raw corncobs, pass through a 30-mesh sieve, and set aside.

[0035] 2) Weigh the above-mentioned crushed corncobs, add the zinc chloride activator solution with a mass fraction of 40% (mass ratio of corncobs to zinc chloride solution is 1:3), stir and mix, and mix the mixed The feed liquid is soaked at room temperature for about 1 hour;

[0036] 3) Put the impregnated material liquid into a high-temperature muffle furnace, raise it from room temperature to the required activation temperat...

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Abstract

A preparation method for an immobilized microbe oil-spill repairing agent is characterized by comprising the following steps: preparing corncob-based active carbon; mixing the corncob-based active carbon with a seed bacteria liquid to performing adsorption until adsorption is saturated, so as to form a bacteria suspension; adding a sterilized sodium alginate solution with a temperature of 30-40 DEG C into the above bacteria suspension, uniformly mixing, injecting into a CaCl2 solution with a certain concentration by employing an injector, dispersing into balls and performing crosslinking for 12-24 h, so as to obtain immobilized microbe microballoons; and finally flushing the immobilized microbe microballoons with normal saline for 2-4 times to obtain a finished product. According to the preparation method, the biology-based active carbon modified corncob is added into a sodium alginate-calcium chloride embedded immobilized carrier for the first time, the mechanical strength and the mass transfer property of the agent are improved, the activity and the degradation efficiency of microbes are improved; also the cost is low, the technology is simple and easy to operate, the reaction conditions are mild, microbes of the prepared repairing agent is not easy to leak, and the prepared repairing agent is good in stability and reusability, has relatively high microbe activity and cell capacity, and has petroleum hydrocarbon degradation rate of 78-88%.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of petroleum and petroleum product pollution, and specifically relates to a preparation method of an immobilized microbial oil spill restoration agent using sodium alginate-calcium chloride-corncob-based activated carbon as an embedded immobilization material. Microbial remediation of oceans polluted by petroleum and petroleum products. Background technique [0002] With the development of offshore oil development and marine transportation, oil spill accidents caused by blowouts, oil spills from transport ships, shipwrecks, and oil pipeline leaks have occurred frequently. After the oil spill accident, although some surface oil spills can be removed by physical methods (such as fence recovery, etc.) and chemical methods (spraying oil dispersants, etc.), the remaining petroleum hydrocarbons remain in the marine environment for a long time, which is harmful to marine ecosystems and Human heal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C02F3/34C01B31/08C12R1/01C02F101/32C02F103/08C01B32/324C01B32/348
Inventor 陈庆国刘梅包木太胡海燕
Owner ZHEJIANG OCEAN UNIV
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