Preparation method of yeast extract

A technology of yeast extract and yeast slime, which is applied in the field of improving the yield of yeast extract preparation, and can solve the problems of long time autolysis, high cost, and high price

Active Publication Date: 2014-07-16
HANGZHOU JINGYINKANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the thick cell wall of yeast, autolysis takes a long time and the yield is low
[0005] (2) Enzymatic hydrolysis method, in addition to the various enzymes contained in the yeast itself, exogenous proteases are added to make the yeast release the contained substances and decompose them into small molecular sugars, amino acids, peptides, etc. The yeast extract obtained by the enzymatic hydrolysis method The quality of the item is good, but the cost is high
[0009] Sulfhydryl proteases from plants, such as papain, cannot be expressed and produced by recombinant genetic engineering technology at present, and can only be extracted from papaya, which is very expensive and takes up a lot of farmland for planting, wasting natural resources

Method used

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  • Preparation method of yeast extract
  • Preparation method of yeast extract
  • Preparation method of yeast extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1, N-sugar amidase F enzymatic hydrolysis prepares yeast extract

[0058] Dilute 20 g of yeast slime to 100 ml with 50 mM pH7.5 sodium phosphate buffer, and suspend the yeast slime in sodium phosphate buffer; obtain 20% yeast liquid.

[0059] Add 0.5 g of N-glycosidase F (that is, the concentration is 0.5%) to the above 100 ml of 20% yeast liquid, and perform enzymatic hydrolysis at 37° C. and 150 rpm for 20 hours. Then add 1g of sodium chloride (that is, the concentration of sodium chloride is 1%), adjust the pH to 6.0 with 1M hydrochloric acid; autolyze at a constant temperature of 50°C for 20 hours. After constant temperature autolysis, inactivate the enzyme at 90°C for 20 minutes, then centrifuge at 3800rpm for 15 minutes, collect the supernatant, and dry the supernatant at 105°C to constant weight to obtain yeast extract.

[0060] Experiment 1. Calculation of Yeast Extract Yield

[0061] The collected supernatant was placed in an oven (105°C) to consta...

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Abstract

The invention discloses a preparation method of a yeast extract. N-PNGase F is selected and used in the preparation method. The preparation method comprises the following steps: (1) adding 50mM of a sodium phosphate buffer solution with the pH value of 7-8 into 19g-21g of yeast paste to make up the volume to be 100ml; suspending the yeast paste in the sodium phosphate buffer solution to obtain a yeast solution; and (2) adding 0.5g-1.0g of N-PNGase F into the yeast solution, enzymatically hydrolyzing at the speed of 120-180rpm and the temperature of 36.5-37.5 DEG C for 16-24 hours, adding 0.8g-1.2g of sodium chloride, adjusting the pH value to be 6.0, autolyzing at the constant temperature of 45-55 DEG C for 18-28 hours, performing enzyme deactivation, centrifuging at the speed of 3,500-4,000rpm for 13-17 minutes, collecting supernatant liquid, and drying to the constant weight at 105 DEG C to obtain the yeast extract.

Description

technical field [0001] The invention relates to a method for improving the yield of yeast extract. Background technique [0002] Yeast extract is made from yeast cells (such as brewer's yeast, Saccharomyces cerevisiae and Candida utilis, etc.), using biotechnology to autolyse, separate, vacuum concentrate, spray-dry the protein and nucleic acid in yeast cells, etc. The refined products are rich in protein, amino acid, polypeptide, nucleotide, B vitamins, growth factors, trace elements, etc. Yeast extract is widely used as a non-animal-derived product in growth media for bacteria and fungi, as well as serum-free culture media for mammalian and insect cells, and as a water-soluble vitamin in many other media formulations. Nutritional source of organisms, amino acids, peptides and carbohydrates. These media are widely used in the fields of biochemical culture and biopharmaceuticals, and have high product added value. In the food processing industry, yeast extract is an inter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A23L1/221A23L27/10
Inventor 姜有为
Owner HANGZHOU JINGYINKANG BIOTECH CO LTD
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