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Method for preparing oil absorbing and degrading material by waste keratins

A keratin and lysate technology, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of slow growth, short survival period, limited application, etc., to improve growth efficiency and cost. low effect

Inactive Publication Date: 2014-07-23
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the small size of the bacteria, poor mobility, short survival period of simple bacteria in the natural environment of oil pollution, slow growth, poor autonomy in practical application, and slow contact with the target limit the practical application of this process (Wang et al. 2013)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 0.5kg of hair, wash it with detergent, crush it, put it into 2.5kg of solution (8M urea, 0.1M sodium metabisulfite and 0.5M sodium dodecylsulfate (SDS)), 80°C, 70r / The speed of min was shaken to dissolve for 40 min. The protein solution was placed at room temperature for cross-linking, and then filtered and dried. Take 100 grams of the cross-linked sample and add 1000 mL of bacterial (Escherichia coli1098-3, CGMCC No.8696) culture solution, put it in a shaker, 20 ° C, 120 r / min immobilization for 24 hours, filter to obtain the finished product of oil-absorbing sponge immobilized bacteria, 20 ° C Protect from light and dry. Put the dried sample into 100 kg of water with 100 g of kerosene on the surface, and after 24 hours, the residual oil basically disappears.

Embodiment 2

[0023] Take 0.5 kg of feathers, wash them with detergent, crush them, put them into 5 kg of solution (4M urea, 0.5M sodium metabisulfite and 0.3M sodium dodecylsulfate (SDS)), 90°C, 120r / min Shake at a high speed to dissolve for 30 minutes. The protein solution was placed at room temperature for cross-linking, and then filtered and dried. Take 100 grams of cross-linked samples and add 1000 mL of bacteria (Pseudomonas mendocina Palleroni, CGMCC No.1.871) culture solution, place in a shaker, 30 ° C, 150 r / min immobilization for 20 h, filter to obtain the finished product of oil-absorbing sponge immobilized bacteria, 30 ° C to avoid Light dry. Put the dried sample into 100kg of water with 100g of diesel oil on the surface. After 48 hours, the residual oil basically disappeared.

Embodiment 3

[0025] Take 0.5 kg of wool, wash it with detergent, crush it, put it into 10 kg of solution (0.5M urea, 0.01M sodium metabisulfite and 0.05M sodium dodecylsulfate (SDS)), 100°C, 200r / The speed of min was oscillated to dissolve for 30 min. The protein solution was placed at room temperature for cross-linking, and then filtered and dried. Take 100 grams of the cross-linked sample and add 1000 mL of bacterial (Escherichia coli1098-3, CGMCC No.8696) culture solution, place in a shaker, 20 ° C, 120 r / min immobilization for 24 hours, filter to obtain the finished product of oil-absorbing sponge immobilized bacteria, 30 ° C Protect from light and dry. Put the dry sample into 100kg of water with 100g of kerosene and kerosene mixed on the surface. After 50 hours, the residual oil basically disappears.

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PUM

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Abstract

The invention relates to a method for preparing oil absorbing and degrading material by waste keratins. The method comprises the following steps: (1) cleaning and drying the waste keratins to obtain clean objects, then, cutting the clean objects and then adding the cut objects into a solution to be dissolved, and filtering to remove residual slags to obtain a keratin solution; and (2) placing the keratin solution at room temperature to crosslink, filtering and drying to obtain a sponge sample, and adding a bacterial culture medium to immobilize bacteria to obtain the oil absorbing and degrading material. The method provided by the invention is low in cost,environmental friendly and free from secondary pollution in the technical process, has very significant scientific meaning and application value to the present world short of resources and energy resources, and further meets the concept of circular economy and the strategic demand of a conservation-minded society.

Description

technical field [0001] The invention belongs to the field of water environment cleaning, and in particular relates to a method for preparing an oil-absorbing and de-oiling material by using waste keratin. Background technique [0002] Oil and its products entering the marine environment during mining, refining, storage and use will cause serious pollution; among them, oil spill pollution is the most harmful, and oil spills are known as the super killer of marine pollution. The world's oil spilled into the ocean due to tanker accidents is about 390,000 tons per year (Wang Chuanyuan et al., 2009). In recent years, China has discharged about 120,000 tons of oil into the sea every year, and the average mass concentration of oil in China's offshore waters has reached 0.055mg / L, and the pollution is increasing day by day. In addition to nutrients, petroleum hydrocarbons have become the main pollutants in the world's oceans, especially shallow seas (Al-Majed et al. 2012). With th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02C02F3/34C12R1/19C12R1/38
Inventor 曹张军雷晓辉周美华
Owner DONGHUA UNIV
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