Refreshing agent and method for inhibiting blackening of prawns
A preservative and shrimp technology, which is applied in the field of preservatives for inhibiting melanization of shrimp, can solve the problem that the effect of inhibiting melanization is not ideal, and achieve the effects of maintaining color, ensuring safety, and extending shelf life
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Embodiment 1
[0021] 1) Extract kelp polyphenols
[0022] Weigh 20 g of kelp, wash it twice with distilled water, add 800 mL of ethanol with a concentration of 80wt% after pulverizing the kelp, extract under microwave-assisted conditions, the microwave power is 650 W, and the extraction time is 80 s, filter, and the filtrate is evaporated by rotary evaporation Concentrate, then purify with XDA-1 macroporous adsorption resin, elute with 80% ethanol by mass percentage, concentrate the purified solution by rotary evaporation, pre-cool at -80°C for 6h, take it out and place it in the freezer Freeze-dried in a drier for 24 hours, and the extraction rate of kelp polyphenols was 2.14%; the content of kelp polyphenols was determined by the Folin phenol method, and the content of polyphenols in the prepared kelp polyphenols was 83.27%;
[0023] 2) Extract laminarin
[0024] Weigh 20 g of kelp, wash it twice with distilled water, dry at 105 °C for 20 min, grind the dried kelp into kelp powder, add 8...
Embodiment 2
[0030] 1) Extract kelp polyphenols
[0031] Weigh 20g of kelp, wash it with distilled water for 5 times, add 1200 mL of ethanol with a concentration of 70wt% after crushing the kelp, extract under microwave-assisted conditions, the microwave power is 750W, the extraction time is 60s, filter, and use rotary evaporation to concentrate the filtrate , then purified with XDA-1 macroporous adsorption resin, eluted with 70% ethanol by mass percentage, concentrated the purified solution by rotary evaporation, pre-cooled at -80°C for 6h, took it out and placed it in freeze-drying Freeze-dried in the machine for 24 hours, and the extraction rate of kelp polyphenols was 2.47%; the content of kelp polyphenols was determined by the Folin phenol method, and the content of polyphenols in the prepared kelp polyphenols was 83.57%;
[0032] 2) Extract laminarin
[0033] Weigh 20 g of kelp, wash it with distilled water for 5 times, dry it at 105 °C for 20 min, grind the dried kelp into kelp pow...
Embodiment 3
[0039] 1) Extract kelp polyphenols
[0040]Weigh 20g of kelp, wash it with distilled water for 3 times, add 1000mL of ethanol with a concentration of 75wt% after pulverizing the kelp, extract under microwave-assisted conditions, the microwave power is 700W, the extraction time is 70s, filter, and use rotary evaporation to concentrate the filtrate, Then purify with XDA-1 macroporous adsorption resin, elute with 75% ethanol by mass percentage, concentrate the purified solution by rotary evaporation, pre-cool at -80°C for 6 hours, take it out and place it in a freeze dryer Freeze-dried in medium for 24 hours, the extraction rate of kelp polyphenols was 2.73%; the content of kelp polyphenols was determined by the Folin phenol method, and the content of polyphenols in the prepared kelp polyphenols was 84.09%;
[0041] 2) Extract laminarin
[0042] Weigh 20 g of kelp, wash it with distilled water three times, dry it at 105 °C for 20 min, grind the dried kelp into kelp powder, add 1...
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