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Method for detecting biogenic amine in yellow wine by using chromatographic sheet

A thin-layer chromatography plate and thin-layer chromatography technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of expensive, unable to meet high-throughput, cheap, simple, fast detection requirements, time-consuming, etc., to reduce The effect of detection cost, shortened detection time, and easy operation

Inactive Publication Date: 2014-08-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the high-performance liquid chromatography technology is accurate and sensitive, it is expensive and time-consuming, and can only complete the detection of one sample at a time, which is far from meeting the high-throughput, cheap, simple and rapid detection requirements in actual production.

Method used

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  • Method for detecting biogenic amine in yellow wine by using chromatographic sheet
  • Method for detecting biogenic amine in yellow wine by using chromatographic sheet
  • Method for detecting biogenic amine in yellow wine by using chromatographic sheet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The making method of embodiment 1 different concentration standard solution standard curve

[0035] The standard curve of different biogenic amines was prepared by thin-layer chromatography, including the following steps:

[0036] (1) Preparation of biogenic amine standard solutions of different concentrations: 10-400 μg / mL tyramine and spermidine standard solutions; 5.0-200 μg / mL spermine standard solutions; 20-800 μg / mL histamine and putrescine standard solutions; 40-1000μg / mL cadaverine standard solution. Take standard solutions of different concentrations in different plastic centrifuge tubes, first add saturated NaHCO with the same volume as the standard solution 3 Make the solution alkaline, then add dansyl chloride derivative solution (1-10mg / mL acetone) 1-5 times the volume of the standard solution, vortex and mix on a vortex mixer for 1min, then place in the dark at 30°C After derivatizing in a constant temperature water bath at -60°C for 10-60 minutes, take ...

Embodiment 2

[0041] Example 2 Detection of biogenic amines in rice wine samples derivatized at different derivatization temperatures

[0042] Thin-layer chromatography qualitatively and quantitatively detects biogenic amines in rice wine samples derived at different derivatization temperatures, including the following steps:

[0043] (1) Take rice wine samples respectively in different plastic centrifuge tubes, first add saturated NaHCO of the same volume as the rice wine sample solution 3 Make the solution alkaline, then add dansyl chloride derivative solution (1-10 mg / mL acetone) 1-5 times the volume of the sample solution, vortex and mix on a vortex mixer for 1 min, and place in dark room temperature, After derivatizing in a constant temperature water bath at 35°C, 45°C, 50°C, 55°C, and 60°C for 30 minutes, take it out, place it at room temperature for a few minutes, add 1 / 2 of the sample solution volume of saturated NaCl solution, and then add the sample solution Equal volume organic ...

Embodiment 3

[0049] Example 3 Detection of biogenic amines in rice wine after different derivation times

[0050] The method for detecting biogenic amines in rice wine after derivatization for different time by thin layer chromatography comprises the following steps:

[0051] (1) Take rice wine samples of different concentrations in different plastic centrifuge tubes, first add saturated NaHCO of the same volume as the rice wine sample solution 3 Make the solution alkaline, then add dansyl chloride derivative solution (1-10mg / mL acetone) 1-5 times the volume of the sample solution, vortex and mix on a vortex mixer for 1min, and place them in the dark at a constant temperature of 60°C Derivatize in a water bath for 10min, 20min, 30min, 40min, 50min, and 60min respectively, take it out, place it at room temperature for a few minutes, add 1 / 2 of the sample solution volume of saturated NaCl solution, and then add an organic solvent equal to the volume of the sample solution Extraction, after ...

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Abstract

The invention discloses a method for detecting biogenic amine in yellow wine by using a chromatographic sheet, belonging to the technical field of analysis and detection of components in the yellow wine. The method comprises the steps of carrying out derivation on a biogenic amine standard substance and a to-be-tested yellow wine sample by using a derivatization reagent, enriching the biogenic amine by using liquid-liquid extraction, carrying out sample application on a sheet plate, arranging the sheet plate in a saturate tank loaded with a certain proportion of developing solvent and unfolding; developing and observing under an ultraviolet lamp, and qualitatively detecting the target biogenic amine; and finally, quantitatively measuring the biogenic amine in the yellow wine by using a thin layer chromatograph. According to the method, the detection limit range of the biogenic amine is 0.5-5 mug / mL, the average adding standard recovery rate is 88.5-110.3 percent, the standard deviation is 3.8-12.5 percent, and at most 13 samples can be simultaneously detected on a 20*20 thin layer glass plate. Compared with other analysis methods, the method has the advantages that qualitative and quantitative analysis of the biogenic amine in the yellow wine can be realized, the operation is simple and convenient, the cost is low, and a plurality of samples can be simultaneously detected.

Description

technical field [0001] The invention relates to a method for detecting biogenic amines in rice wine by using a thin-layer chromatography plate, in particular to a method for qualitatively and quantitatively detecting the types and contents of biogenic amines in rice wine by using thin-layer chromatography, and belongs to the technical field of analysis and detection of rice wine components. Background technique [0002] Rice wine is a unique fermented wine in my country, and it can be called a typical representative of the oriental brewing industry. But its fermentation process is complex and difficult to control, so rice wine liquor contains rich and complex components, including water, ethanol, sugars, organic acids, nitrogenous compounds, total solids, non-volatile esters, volatile components (including alcohol , acids, esters, hydroxyl compounds), organic components, etc. There are as many as 18 kinds of amino acids contained in it, and the total content is one to sever...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 毛健黄桂东魏晓璐
Owner JIANGNAN UNIV