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Method for preparing high-purity galactooligosaccharide through synergy of enzymes

A high-purity galacto-oligosaccharide technology, applied in the field of preparation of galactooligosaccharides, can solve the problems of high preparation cost and low purity of high-purity galacto-oligosaccharides, and achieve improved purity and high tolerance. The effect of high removal rate of monosaccharide and monosaccharide

Active Publication Date: 2014-08-20
SHANDONG LONGLIVE BIO TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the above-mentioned deficiencies and provide a method for synergistically preparing high-purity galacto-oligosaccharides with enzymes, thereby solving the problems that the purity of galacto-oligosaccharides is generally low and the production cost of high-purity galacto-oligosaccharides is high

Method used

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  • Method for preparing high-purity galactooligosaccharide through synergy of enzymes

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Weigh 50g of lactose and add 50g of water to configure 100g of 50% lactose solution, adjust the pH value of the sugar solution to 5.0 with alkali; add 20U / g of β-glucosidase B and 20U / g of β-galactosidase A, The enzyme activity ratio of β-galactosidase A and β-glucosidase B is 1:1; it is maintained at 65°C for 10 minutes, and converted at 45°C for 4 hours, 62.53% galactooligosaccharides, 2.03% lactose, galactose 9.11%, glucose 26.33%. Water bath at 100°C for 5 minutes. After inactivating the enzyme, take 5g of sugar solution and divide it into I solution and II solution. Take 1g of I solution and dilute it with water to 2g (the sugar concentration at this time is about 20%). Add 0.01g of dry yeast powder. Activated at 34°C for 2 hours, then transferred to 4g of II solution and cultured for 6 hours, then directly transferred to the remaining sugar solution, and fermented for 48 hours. Glucose 0.5%. Decolorization: Add activated carbon to the fermented sugar solution fo...

Embodiment 2

[0029] Dissolve lactose in water, heat to dissolve to control the mass concentration of lactose at 60%, adjust the pH of the sugar solution to 5.5 with hydrochloric acid or sodium hydroxide; add β-galactosidase A and 6U / g β-glucose in an amount of 8U / g The enzyme activity ratio of glucosidase B, β-galactosidase A and β-galactosidase B was controlled at 1.33:1; kept at 50°C for 15 minutes, then cooled to 45°C for 6 hours. After conversion, the composition of the sugar liquid is: 55.96% of galacto-oligosaccharides, 9.15% of lactose, 7.31% of galactose, and 29.15% of glucose.

[0030] Enzyme inactivation: 100°C water bath for 10 minutes, then cool to room temperature.

[0031] Preparation of hyperosmotic Saccharomyces cerevisiae: Take 5g of enzyme-inactivating sugar solution, divide it into I solution and II solution, take 2g of I solution, dilute 1.5 times to 5g (sugar concentration is about 20%), add 0.05g of yeast dry powder, and keep at 32°C After 4 hours of lower fermentati...

Embodiment 3

[0038] Weigh 30g of lactose and add 70g of water to configure 100g of 30% lactose solution, adjust the pH value of the sugar solution to 5.0 with hydrochloric acid; add 20.0U / g of β-galactosidase A and 0.4U / g of β -Galactosidase B, the enzyme activity ratio of β-galactosidase A and β-galactosidase B is controlled at 50:1; keep at 55°C for 15 minutes, then lower the temperature to 40°C for 8 hours, boil for 5 minutes Inactivate enzymes. Take 5mL of sugar solution and divide it into liquid I and liquid II. Take 1g of liquid I, dilute it twice to 2g (sugar concentration is about 15%), add 0.004g dry yeast powder, activate at 34°C for 4h, and then transfer to 4g of II After being cultured in the liquid for 6 hours, it was directly transferred to the remaining transformation liquid, and fermented for 16 hours. The results of transformation and fermentation are shown in Table 1.

[0039] Table 1 β-galactosidase separate transformation and embodiment 3 synergistic transformation eff...

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Abstract

The invention relates to a method for preparing high-purity galactooligosaccharide through synergy of enzymes. The method comprises the steps of adding water into lactose, heating up for dissolving, and regulating the pH value to be 4.5-7.0; adding beta-galactosidase A and beta-glucosidase B into the lactose solution, converting for 10-30 minutes at 50-70 DEG C firstly, then keeping for 4-24 hours at 35-55 DEG C; deactivating the enzyme, cooling down to room temperature, adding highly-hypertonic resisting brewer yeast into the liquid glucose with the enzyme deactivated, and reacting for 12-24 hours at 35 DEG C; and decoloring the product, performing ion exchange, concentrating, spray drying so as to obtain the high-purity galactooligosaccharide. According to the method, two enzymes are simultaneously used to produce the galactooligosaccharide, so that the purity of the galactooligosaccharide is high, the content of the galactooligosaccharide is significantly improved, and the content of lactose is lowered.

Description

technical field [0001] The invention relates to a preparation method of galacto-oligosaccharides, in particular to a method for preparing high-purity galacto-oligosaccharides by using enzymes to co-convert lactose. Background technique [0002] Galactooligosaccharides (Galactooligosaccharides, GOS) is a functional polysaccharide with natural properties, and its molecular structure is generally 1 to 7 galactosyl groups connected to galactose or glucose molecules, that is, Gal-(Gal)n -Glc / Gal (n is 0-6). In nature, there are trace amounts of GOS in animal milk, but more in human breast milk. The establishment of bifidobacteria in infants largely depends on the GOS components in breast milk. Galacto-oligosaccharides have the functions of specifically promoting the proliferation of intestinal bifidobacteria, indigestion and low energy, reducing and preventing constipation, low caries, improving mineral absorption, lipid metabolism, and enhancing immunity. In addition, galactoo...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/12C12P19/04C12R1/865
Inventor 肖林夏蕊蕊覃树林司立萍
Owner SHANDONG LONGLIVE BIO TECH CO LTD
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