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Method for preparing and purifying naringin

A purification method and naringin technology are applied in the field of preparation and purification of naringin, which can solve the problems of long production process and high cost, and achieve the effects of low production cost, economic value creation and simple process.

Inactive Publication Date: 2014-09-03
QIANJIANG GREEN SEA TREASURE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The production process is longer and the cost is higher

Method used

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  • Method for preparing and purifying naringin

Examples

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Effect test

example 1

[0018] Chromatographic conditions for naringin analysis: chromatographic column (XDB-C18, 4.6 mm×150 mm, 5 μm); mobile phase (methanol:water:phosphoric acid=35:65:0.2); flow rate 1.0 ml / min; column oven ( 30°C); injection volume 20 μl; detection wavelength (λ=283 nm).

[0019] Reference substance solution preparation: Accurately weigh 25.0 mg of naringin reference substance in a 100 ml volumetric flask, add 1 ml of methanol to dissolve, then add water to the mark, shake well, take 1-2 ml and filter with a 0.45 μm microporous membrane to make It is the naringin reference solution.

[0020] Raw material solution preparation: Accurately weigh three parts of about 0.5 g of raw material and place them in a 250 ml corked Erlenmeyer flask, accurately add 100.0 ml of 50% ethanol (volume fraction), seal the plug, and weigh it. Soak the triangular flask in a 60°C water bath for 30 minutes, shake well, and ultrasonically shake at room temperature for 30 minutes, take out and dry the bot...

example 2

[0026] Crush the young pomelo fruit to about 10 meshes, weigh 40g into a 250ml Erlenmeyer flask with a stopper, add 100ml of water, seal it tightly, take a water bath at 95°C for 60 minutes, filter, and extract the filter residue once again with 100ml of water; combine the filtrates in In a 500ml beaker, cool to 30°C with a tap water bath, add a small amount of naringin crystals and a magnetic rotor, stir on a magnetic stirrer at 100r / min for 2 hours, a large amount of crystals precipitate, filter, and dry to obtain 2.43g of light yellow powder The solid was 94% pure by HPLC analysis.

example 3

[0028] Take 200kg of pomelo young fruit, crush it, pour it into the extraction kettle, inject 400kg of water, heat it with steam to 90°C, keep it warm for 1h, release the extract, and extract the filter residue twice with 400kg of water; Filtrate the liquid while it is hot, transfer the filtrate to the extraction kettle again, cool it to 35°C with cooling water, add 2g of naringin crystals, stir and crystallize for 4h, stop stirring to allow the crystals to settle, release the supernatant from the side hole, and put the crystallization liquid in the lower layer Put it into a three-legged centrifuge, dehydrate, and wash with a small amount of water to obtain about 20kg of moist light yellow solid, dissolve it again in 100kg of hot water at 90°C, filter it with a diatomaceous earth filter while it is hot, and cool it to 30°C with cooling water under constant stirring. ℃, a large amount of white crystals precipitated, continued stirring for 1 hour, poured the crystalline solution ...

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Abstract

The invention discloses a method for preparing and purifying naringin, namely a method for extracting naringin from young fruits of citrus paradisi macfadyen by adopting a hot water extraction and cooling crystallization process and purifying the naringin. The method is characterized by comprising the steps of collecting young citrus paradisi macfadyen fruits of which the diameters are less than 5cm, pulverizing, and extracting with hot water; filtering the extract, cooling and adding seed crystal for crystallizing; removing the mother solution, dissolving the coarse crystal into hot water again, cooling and re-crystallizing to obtain almost white crystal powder, wherein the content of the naringin is more than 96% through HPLC (high performance liquid chromatography) detection. Compared with the present naringin production method, the method has the advantages that the process is simple, the production cost is low, and the waste young fruits are comprehensively utilized to create economic value.

Description

technical field [0001] The invention relates to a method for preparing and purifying naringin, in particular to a method for extracting, preparing and purifying naringin from the young fruit of pomelo, a Rutaceae plant, by using a hot water extraction-cooling crystallization process. Background technique [0002] Naringin (Naringin, Cas 10236-47-2) is a flavanone glycoside compound widely present in the fruits and peels of pomelo, grapefruit, lime and other plants of Rutaceae. Naringin has biological activities such as lowering blood fat, anti-oxidation, anti-tumor, anti-fungal, and anti-atherosclerosis. It can be used to produce drugs or health care products for cardiovascular diseases, anti-inflammatory and sore treatments, and can also be used as an additive for oral care products . In addition, using naringin as raw material, naringin dihydrochalcone, a new sweetener with cool taste, high sweetness and low energy, can be synthesized through alkalization, ring opening a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 林晓锋王国艳陈琰
Owner QIANJIANG GREEN SEA TREASURE BIO TECH
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