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A kind of salt-tolerant methylotrophic bacillus strain lj and its application

A methylotrophic, bacillus technology, applied in the field of microorganisms, can solve the problems of low viable bacteria, inability to grow, poor persistence and stability, etc., and achieve high effective viable bacteria rate, long shelf life, and improved yield Effect

Active Publication Date: 2016-03-02
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In arid and semi-arid areas, secondary salinization of soil caused by long-term drip irrigation, excessive and low-efficiency use of chemical fertilizers, and serious soil-borne diseases are prominent problems in crop seedling production, as well as problems in the production and application of agricultural microbial agents. The number of viable bacteria is low, the function is single, and the persistence and stability are poor. Therefore, it is necessary to use the interaction between microorganisms and plants in special environments as a way for plants to tolerate salt, prevent diseases, and promote growth. Research on resource excavation and organization, biological characteristics and mechanism of action of bacteria and disease-preventing and growth-promoting bacteria
In the face of the increasingly serious soil salinization phenomenon, the isolation of salt-tolerant genes, the construction of transgenic salt-tolerant plants, and the development and utilization of saline-alkali soils are research directions that are closely watched by countries all over the world. Salt-tolerant bacteria are also called facultative halophilic bacteria. It grows and reproduces in environment and salt-free environment, and its salt resistance can be as high as 20%. Its main feature is facultative halophilia, and it can grow in a wide range of environments. It is a rich source of genes for salt-tolerant bacteria. Although it grows in extreme environments Some extreme halophilic bacteria in the environment have high tolerance to salinity, but they cannot grow under low-salt or no-salt conditions

Method used

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  • A kind of salt-tolerant methylotrophic bacillus strain lj and its application
  • A kind of salt-tolerant methylotrophic bacillus strain lj and its application
  • A kind of salt-tolerant methylotrophic bacillus strain lj and its application

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Embodiment 1

[0019] 1. Isolation and screening of salt-tolerant strains

[0020] On July 4, 2012, 24 soil samples were collected from the rhizosphere soil of Tobacco Plants in Binzhou, Shandong, and 1g of each soil sample was suspended in 100ml sterile water and diluted to 10 5 times. Use Gibbons improved medium or beef extract peptone medium for screening, Gibbons improved medium formula: casein hydrolyzate 5.0g, sodium citrate 3.0g, yeast extract powder 10.0g, KCl2.0g, peptone, 5.0g, MgSO 4 ·7H 2 O, 20gNaCl100g, pH7.0. Use 1%, 4%, 7%, 10% NaCl to screen the salt-tolerant strains step by step. Use a pipette to take 0.1ml of the soil dilution solution and spread it on the two medium plates, incubate at 30°C for 2 to 3 days, select colonies that grow well at 10% salt concentration, and save them for future use after repeated streaking and purification. to a salt-tolerant strain LJ.

[0021] 2. Identification of the salt-tolerant strain LJ

[0022] The screened strain LJ was cultured o...

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Abstract

The invention provides a salt-resistance Bacillusmethylotrophicus strain LJ and an application thereof. The salt-resistance Bacillusmethylotrophicus strain LJ is classified to be named as Bacillusmethylotrophicus, and the collection number of the salt-resistance Bacillusmethylotrophicus strain LJ is CGMCCNO.7570. By performing salt tolerance analysis on the salt-resistance Bacillusmethylotrophicus strain LJ, performing a pathogenicity test on crops, and performing a test on the inhibition effect of the strain on pathogenic bacteria, results show that the salt tolerance degree of the strain is 25-30%; the strain can not cause pathogenicity to the crops, has relatively ideal inhibition effects on bacterial diseases, viral diseases and fungous diseases of tobaccos, can be compounded with a great number of elements, is long in retention period and high in effective bacterium survival rate, can be applied to saline-alkali soils, and has the effects of improving the soil, resisting the diseases, increasing the yield and the like. The salt-resistance Bacillusmethylotrophicus strain LJ disclosed by the invention ensures that a microbial preparation has the positive effects of preventing the diseases and promoting the growth of plants in special environments; the microbial inoculum has the effects of salt-resistant growth-promoting bacteria and disease-prevention growth-promoting bacteria, and is suitable for improvement of salt marsh, alkali soil and acid soil.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a salt-tolerant methylotrophic bacillus strain LJ and its application. Background technique [0002] In arid and semi-arid areas, secondary salinization of soil caused by long-term drip irrigation, excessive and low-efficiency use of chemical fertilizers, and serious soil-borne diseases are prominent problems in crop seedling production, as well as problems in the production and application of agricultural microbial agents. The number of viable bacteria is low, the function is single, and the persistence and stability are poor. Therefore, it is necessary to use the interaction between microorganisms and plants in special environments as a way for plants to tolerate salt, prevent diseases, and promote growth. Resource excavation and sorting, biological characteristics and mechanism of action of bacteria and disease-preventing and growth-promoting bacteria. In the face of the inc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P1/00C05F11/08B09C1/10C12R1/07
Inventor 单俊伟翟熙伦王海华
Owner 青岛海大生物集团股份有限公司
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