Method for producing mannitol by taking sugarcane juice as raw material through leuconostoc mesenteroides fermentation

Abstract

The invention discloses a method for producing mannitol by taking sugarcane juice as a raw material through leuconostoc mesenteroides fermentation, relating to a preparation method of mannitol. The method comprises the steps of centrifuging and filtering the squeezed sugarcane juice, and adding brown sugar to obtain sugarcane juice and brown sugar filtrate; then, carrying out cold sterilization preparation to obtain sterile sugarcane juice and brown sugar filtrate as a culture medium for later use; adding activated leuconostoc mesenteroides into the sterile sugarcane juice and brown sugar filtrate culture medium at the inoculation rate of 1%; next, culturing to obtain a fermentation solution, separating and purifying to obtain mannitol. The method disclosed by the invention can be used for reducing the cost of the culture medium, so that the bottleneck problem caused by producing mannitol by using a microbiological fermentation method is solved.

Description

technical field [0001] The technical scheme of the present invention relates to a preparation method of mannitol, specifically, a method for producing mannitol by fermenting leuconostoc enterococci with sugarcane juice as a raw material. Background technique [0002] Mannitol is widely used in pharmaceutical, food, chemical and electronic industries. Global mannitol consumption accounts for about 11% of the total polyols, about 150,000 tons per year. The United States is the largest consumer of mannitol. Others, such as European countries, use a large amount for the production of chewing gum, buccal tablets and chewable tablets. my country's current consumption of mannitol is mainly used directly in the production of mannitol injections, pharmaceutical auxiliary additives and sugar-free food additives. Due to the rapid increase in the demand for mannitol in the food industry, especially chewing gum, the market capacity of mannitol has expanded rapidly. Mannitol is used i...

AI Technical Summary

Problems solved by technology

[0004] (1) Kelp extraction method: about 13-15 tons of dried kelp is needed to extract 1 ton of mannitol. The production process produces a large amount of waste water, high energy consumption, serious pollution, and low yield;

[0005] (2) Catalytic hydrogenation method: Nickel (Ni) catalytic high-temperature and high-pressure hydrogenation with sucrose or glucose as raw material has stable source of raw material, unlimited production period and low cost, but its yield is low, and the associated sorbitol not easy to separate;

[0006] (3) Enzyme conversion method: enzymatic hydrogenation needs to add an expensive coenzyme (NADH) to the system, which is uneconomical;

However, CN201310537851.5 and CN 201410065372.2 still have the defects of relatively high cost of medium, and these defects become the bottleneck problem of producing mannitol by microbial fermentation