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Preparation method of gentamicin sulphate C1a

A technology of gentamicin sulfate and gentamicin, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low bacterial fermentation units, and achieve the production process is simple and easy to operate, fermentation unit high effect

Inactive Publication Date: 2014-09-03
福安药业集团烟台只楚药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The preparation method of existing gentamicin sulfate C1a is: by gentamicin strain mutagenesis to produce the high strain of gentamicin C1a, ferment by this strain, extract gentamicin sulfate C1a, The disadvantage of this method is that the fermentation unit of the strain is low, and the fermentation unit is usually about 995u / ml

Method used

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  • Preparation method of gentamicin sulphate C1a

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Embodiment 1

[0049] The preparation method of gentamicin sulfate C1a comprises the following steps:

[0050] 1) Take gentamicin sandy soil spores and place them on the spore slant medium for spore culture, the culture temperature is 35°C, and the culture time is 8-12 days, and the gentamicin spores after slant culture are obtained;

[0051] The preparation method of gentamicin sand spore is:

[0052] a. The formula of sandy soil spore medium is calculated by weight percentage: soluble starch 1.0%, sodium chloride (NaCl) 0.05%, potassium nitrate (KNO 3 ) 0.1%, calcium carbonate (CaCO 3 ) 0.1%, dipotassium hydrogen phosphate (K 2 HPO 4 ) 0.02%, bran 1.8%, magnesium sulfate (MgSO 4 ) 0.02%, agar 2.0%, and the balance is water; the prepared sandy soil spore medium is divided into 100ml in each eggplant bottle, and the volume of the eggplant bottle is 500ml. 0.1MPa, 30 minutes, 120 degrees;

[0053] b. On the ultra-clean workbench, under the protection of the flame, use a sterile inoculat...

Embodiment 2

[0081] The preparation method of gentamicin sulfate C1a comprises the following steps:

[0082] 1) Take gentamicin sandy soil spores and place them on the spore slant medium for spore culture, the culture temperature is 34°C, and the culture time is 8 days, and the gentamicin spores after slant culture are obtained;

[0083] 2) The gentamicin spores cultured on the slant obtained in step 1) were cultured in shake flasks, and the amount of spores cultured in the shake flasks was: dig 0.3cm from the slant of the spores 2 Insert a shake flask, culture temperature is 38 ℃, culture time is 40 hours, obtains gentamicin shake flask mycelia;

[0084] 3) Place the gentamicin shake flask hyphae obtained in step 2) on the seed medium for seed culture, and the insertion amount of the seed culture is: gentamicin shake flask mycelia per thousand of the volume of the seed medium The second is to insert the shake flask seeds, the culture temperature is 38°C, and the culture time is 48 hours ...

Embodiment 3

[0122] The preparation method of gentamicin sulfate C1a comprises the following steps:

[0123] 1) Take gentamicin sandy soil spores and place them on spore slant medium for spore culture, the culture temperature is 38°C, and the culture time is 12 days, and the gentamicin spores after slant culture are obtained;

[0124] 2) The gentamicin spores cultured on the slant obtained in step 1) were cultured in shake flasks, and the amount of spores cultured in the shake flasks was: dig 0.3cm from the slant of the spores 2 Insert a shake flask, culture temperature is 38 ℃, culture time is 40 hours, obtains gentamicin shake flask mycelia;

[0125] 3) Place the gentamicin shake flask hyphae obtained in step 2) on the seed medium for seed culture, and the insertion amount of the seed culture is: gentamicin shake flask mycelia per thousand of the volume of the seed medium The second is to insert the shake flask seeds, the culture temperature is 38°C, and the culture time is 48 hours to ...

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Abstract

The invention the technical field of antibiotics fermentation, and provides a preparation method of gentamicin sulphate C1a. According to the preparation method, the output of gentamicin C1a is increased by improving the component dosage of a gentamicin fermentation culture medium. The preparation method of the gentamicin sulphate C1a is simple in production process and easy to operate and obtains the high-quality gentamicin sulphate C1a by improving the component dosage and extraction and separation method of the gentamicin sulphate fermentation culture medium.

Description

Technical field [0001] The present invention involves the field of antibiotic fermentation technology, and specifically involves preparation methods for sulfuric acid C1A. Background technique [0002] Antimylinicillin is amino glycoside antibiotics. It has a good antibacterial effect on various Gram -negative bacteria and Gram -positive bacteria.The genus, Salmonella, the genus of Zhiga, the genus Enterococcus, the genus Sandal bacteria, and the copper -green monochrobacterium have good antibacterial effects. The genus Nunase and Bacteria of Bacteria are sensitive to the product.It also has a certain effect on Brucelasia, Glames, Peribrium, and fetal bonus bacteria.About 80%of the metharocyte -sensitive strains of oxygenic oxygenolin (including Golden Polyglycini and Conuselase negatives) have good antibacterial effects, but oxygen Xilin resistant strains are mostly resistant to this product.Poor effects on the genus and pneumonia, pneumonia, and pneumonia. Enterococcus is mostl...

Claims

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Application Information

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IPC IPC(8): C12P19/50C12R1/645
Inventor 葛祥斌王丽丽孙永国马金凤张文浩李贤坤王永盛杨俊法张健
Owner 福安药业集团烟台只楚药业有限公司
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