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A kind of utilization method of indel molecular marker chip

A technology of molecular markers and chips, applied in biochemical equipment and methods, microbial measurement/testing, etc., can solve the problems of backward basic research in molecular biology, and achieve the effect of easy configuration and simple method

Active Publication Date: 2016-03-30
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the basic research on molecular biology of vegetable crops still lags behind the model plant Arabidopsis and food crops rice and corn.

Method used

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  • A kind of utilization method of indel molecular marker chip
  • A kind of utilization method of indel molecular marker chip
  • A kind of utilization method of indel molecular marker chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This example takes the development of Indel molecular markers of cucumber resistance to fusarium wilt and target spot disease into an Indel molecular marker chip and hybridization as an example to describe the utilization method of the present invention in detail. The flow chart of the method can be found in figure 1 .

[0028] 1) Test materials:

[0029] Cucumber materials WF2757 (Material 1) and PL212233 (Material 2) resistant to Fusarium wilt and target spot disease and Jinyan No. 2 (Material 3) susceptible to Fusarium wilt and target spot disease were used as test materials. Among them, WF2757, PL212233 and Jinyan No. 2 were all purchased from Beijing Crop Germplasm Bank.

[0030] 2) Probe design for cucumber disease-resistant Indel molecular marker chip

[0031] According to the results of the positioning research on cucumber resistance to Fusarium wilt and target spot disease (Mao Aijun et al., Inheritance and linkage analysis of cucumber line WIS2757 resistance...

Embodiment 2

[0061] This example verifies the reliability of the present invention with an unknown population. The specific operation is as follows:

[0062] 1) Test materials:

[0063] Using Jinchun 3 and Changchun Mici as parents, construct 600 F 2 genetic population. Among them, Jinchun 3 and Changchun Mici were purchased from Beijing Crop Germplasm Bank.

[0064] 2) Investigation on disease index of cucumber wilt and target spot disease

[0065] The seeds of parents and each group were wrapped with gauze, soaked in warm soup, and then sowed in 50-hole hole trays after germination at a constant temperature of 28°C. Seedlings were raised in an air-conditioned greenhouse, and the seedling substrate was sterilized perlite or nutrient soil. When the cotyledon is fully unfolded and the first true leaf is exposed, it is used for inoculation.

[0066] Cucumber wilt pathogen (Fusarium oxysporum f. sp. cucumerinum Owen.) Physiological Race No. 4 for the test bacterial species is identified ...

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Abstract

The invention discloses a use method of an Indel molecular marker chip. The method comprises the following steps of synthesizing an Indel probe covering whole genome according to the design of an Indel molecular marker; dotting the synthesized Indel probe to a substrate; fixing the Indel molecular marker chip; hybridizing on the fixed Indel molecular marker chip; eluting and scanning the Indel molecular marker chip after the hybridization. The method is simple and easy to realize in a lab, is an open low-cost practical technology system, and can be applied to various aspects of fingerprint identification, gene location, gene pyramiding identifying screening, seed truth identification, genetic background identification, molecular mark assisted breeding and the like; especially, the Indel molecular marker chip designed by the invention can be used for identifying and screening cucumber blight and target leaf spot resistant genes.

Description

technical field [0001] The invention belongs to the field of chip preparation, and in particular relates to a method for utilizing an Indel molecular marker. The utilization method is to design the Indel molecular marker as a probe and manufacture the probe into a chip. Background technique [0002] The prototype of the gene chip (genechip) was proposed in the mid-1980s. It was developed on the basis of gene probes. The so-called gene probes are just a section of artificially synthesized base sequences, and some detectable substances are connected to the probes. , according to the principle of base complementarity, use gene probes to identify specific genes in the gene mixture. It immobilizes a large number of probe molecules on the support, then hybridizes with the labeled sample, and performs analysis by detecting the intensity and distribution of hybridization signals. Gene chips can integrate a large number of molecular detection units on the surface of a tiny solid sub...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6837C12Q2565/501
Inventor 温常龙于拴仓许勇王永勤董从娟
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES