Use of methylene blue in prevention of acute cerebral ischemia damage

A technology of acute cerebral ischemia and methylene blue, applied in the direction of medical preparations containing active ingredients, applications, organic active ingredients, etc., to achieve the effect of improving behavioral ability, good economic and social benefits, and reducing infarct volume

Inactive Publication Date: 2014-09-10
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

However, at present, only about 2% of patients with ischemic stroke can receive thrombolytic therapy in time. Can methylene blue protect the other 98% of patients who miss the golden treatment period and cause permanent neurological damage? The effect has not been reported in the research

Method used

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  • Use of methylene blue in prevention of acute cerebral ischemia damage
  • Use of methylene blue in prevention of acute cerebral ischemia damage

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preparation example Construction

[0016] 10) Preparation of middle cerebral artery occlusive ischemia model: select male SD rats with a body weight ranging from 280 to 350 g, fast 12 hours before the operation, drink water freely, and inject 1% pentobarbital sodium (0.5ml / 100g) After anesthesia by intraperitoneal injection, the rats were fixed on the operating table with the abdomen facing up, the skin was cut from the middle of the neck, and the common carotid artery (CCA), internal carotid artery (ICA) and external carotid artery ( ECA). The thread plug was inserted into the middle cerebral artery through the ECA, the ligature was fastened, and methylene blue was injected intraperitoneally immediately, and the skin was sterilized and sutured.

[0017] Effect verification:

[0018] The neurological function score and cerebral infarction volume measurement are used as methods to verify the therapeutic effect of methylene blue, and the specific implementation is as follows:

[0019] 1) Neurological score

[...

Embodiment 1

[0025] Protective effect of intraperitoneal injection of methylene blue (1mg / kg, 5mg / kg, 10mg / kg) on ​​acute cerebral ischemic injury in MCAO rats

[0026] 1. Materials:

[0027] 1) Pentobarbital sodium (Pelltobarbitalum Natricum): imported from Germany, repackaged by Beijing Reagent Company, batch number 020402, valid until March 2015.

[0028] 2) Preparation of 1% pentobarbital sodium injection: based on the pentobarbital sodium produced by the German Merck company, take 1g of pentobarbital sodium powder, dissolve it in 100ml of normal saline, and mix well to make 1% pentobarbital sodium injection. % Pentobarbital Sodium Injection 100ml, used for animal anesthesia, based on the weight of rats, used according to the dosage of 0.5ml / 100g.

[0029] 3) 0.9% normal saline: produced by Shijiazhuang No. 4 Medicine Co., Ltd., approved by the National Pharmaceutical Association H13023200, valid until January 29, 2015.

[0030] 4) Methylene blue (methylene blue, MB) molecular formul...

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Abstract

The invention relates to novel use of methylene blue (MB) in prevention of acute cerebral ischemia. MB can remarkably reduce cerebral ischemia infarction caused by permanent middle cerebral artery occlusion (middle cerebral artery occlusion MCAO) and alleviate damage of neurological function. The inventors of the invention permanently occlude middle cerebral artery of a mouse by adopting a suture method and after intraperitoneal injection of MB at the moment and at different time points after cerebral ischemia, changes on degree and ethology of permanent cerebral ischemia infarction of the mouse are observed. Researches discover that MB can remarkably reduce infarct volume caused by cerebral ischemia and improve the active ability of the mouse after cerebral ischemia. Through the method provided by the invention, moderate doses of MB are delivered for many times after cerebral ischemia can remarkably alleviate damage degree after permanent cerebral ischemia of the mouse so as to improve the active ability of the mouse after cerebral ischemia. The use of MB is expected to provide a rescue therapeutic measure to cerebral apoplexy and alleviate permanent neurological function deficit after golden treatment period.

Description

technical field [0001] The invention relates to an application of methylene blue against acute cerebral ischemic injury. Immediate intraperitoneal injection of methylene blue after middle cerebral artery occlusion and ischemia in rats, and administration at multiple time points after ischemia, can significantly reduce the volume of infarct foci after permanent cerebral ischemia in rats, and improve the behavioral capacity. Background technique [0002] Ischemic stroke accounts for 70%-80% of the total number of stroke patients, and it is the first cause of adult disability. There are as many as dozens of drugs for the treatment of ischemic stroke, but the current drugs are mainly thrombolytic, and some drugs have serious side effects. Within hours is the golden treatment period), in my country, only 1.6% of patients can receive timely thrombolytic therapy, even in the United States, only 5% of patients can receive timely thrombolytic therapy. Therefore, it is particularly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/5415A61P9/10A23L1/29A23L33/00
Inventor 吴丽颖范明朱玲玲邸瑶赵彤何云凌丁学锋吴奎武黄欣赵永岐刘淑红
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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