Method for preparing thrombin/tannic acid multilayer film compound chitosan hemostatic sponge

A technology of hemostatic sponge and chitosan, applied in medical science, bandages, absorbent pads, etc., can solve the problems of limited immobilization of porous sponges, achieve good clinical application prospects, break through application limitations, and improve storage stability

Inactive Publication Date: 2015-06-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chitosan is usually in the form of microspheres for enzyme immobilization, and porous sponges have limited immobilization

Method used

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  • Method for preparing thrombin/tannic acid multilayer film compound chitosan hemostatic sponge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Dissolve chitosan powder in acetic acid to prepare a chitosan solution with a mass concentration of 1%. After suction filtration, add excess sodium hydroxide aqueous solution until the gel is completely precipitated, and stir to obtain a suspension of gel particles. Rinse with water until neutral, add glycerol solution to plasticize, then centrifuge, pour into a mold, let stand for defoaming, freeze and then freeze-dry to obtain chitosan porous sponge;

[0026] 2) Sterilize the chitosan sponge obtained by freeze-drying by ultraviolet irradiation;

[0027] 3) Dissolve BPEI in phosphate buffered solution PBS, and prepare a BPEI solution with a mass concentration of 1 mg / mL, which is called solution A; dissolve tannic acid in PBS, and prepare a tannic acid solution with a mass concentration of 1 mg / mL, which is called Liquid B: Dissolve thrombin in PBS to prepare a thrombin solution with a mass concentration of 1 mg / mL, called liquid C.

[0028] 4) Soak the chitosan sp...

Embodiment 2

[0036] 1) Dissolve chitosan powder in acetic acid to prepare a chitosan solution with a mass concentration of 3%. After suction filtration, add excess sodium hydroxide aqueous solution until the gel is completely precipitated. Stir to obtain a suspension of gel particles. Rinse with water until neutral, add glycerol solution to plasticize, then centrifuge, pour into a mold, let stand for defoaming, freeze and then freeze-dry to obtain chitosan porous sponge;

[0037] 2) Sterilize the chitosan sponge obtained by freeze-drying by ultraviolet irradiation;

[0038] 3) Dissolve BPEI in PBS, and prepare a BPEI solution with a mass concentration of 2 mg / mL, which is called solution A; dissolve tannic acid in PBS, and prepare a tannic acid solution with a mass concentration of 2 mg / mL, which is called solution B; Dissolve thrombin in PBS, and prepare a thrombin solution with a mass concentration of 2 mg / mL, which is called solution C.

[0039] 4) Soak the chitosan sponge in step 2) i...

Embodiment 3

[0046]1) Dissolve chitosan powder in acetic acid to prepare a chitosan solution with a mass concentration of 5%. After suction filtration, add excess sodium hydroxide aqueous solution until the gel is completely precipitated, and stir to obtain a suspension of gel particles. Rinse with water until neutral, add glycerol solution to plasticize, then centrifuge, pour into a mold, let stand for defoaming, freeze and then freeze-dry to obtain chitosan porous sponge;

[0047] 2) Sterilize the chitosan sponge obtained by freeze-drying by ultraviolet irradiation;

[0048] 3) Dissolve BPEI in PBS, and prepare a BPEI solution with a mass concentration of 3 mg / mL, which is called liquid A; dissolve tannic acid in PBS, and prepare a tannic acid solution with a mass concentration of 3 mg / mL, which is called liquid B; Dissolve thrombin in PBS, and prepare a thrombin solution with a mass concentration of 3 mg / mL, which is called solution C.

[0049] 4) Soak the chitosan sponge in step 2) in...

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Abstract

The invention discloses a method for preparing a thrombin / tannic acid multilayer film compound chitosan hemostatic sponge. The method comprises the following steps: preparing a chitosan porous sponge; assembling the thrombin / tannic acid multilayer film on the chitosan sponge by virtue of a layer by layer self-assembly method; standing the assembled compound sponge overnight for dehydrating and freeze-drying. By regulating the number of the thrombin / tannic acid bilayer, the thrombin drug loading capacity can be regulated and controlled, i.e., the hemostatic effect can be regulated and controlled; compared with the free state thrombin, the thrombin / tannic acid multilayer film compound chitosan hemostatic sponge can be used for greatly improving the storage stability of the thrombin, has the advantages of fast hemostasis speed and good effect, and is expected to be used for war wound and hemostasis of pre-hospital care.

Description

technical field [0001] The invention relates to a preparation method of a chitosan-based hemostatic dressing, in particular to a preparation method of a thrombin / tannic acid multilayer film composite chitosan hemostatic sponge. Background technique [0002] Uncontrolled haemorrhage is an important cause of military casualties and civilian deaths. An ideal emergency hemostatic dressing should meet the following requirements: good hemostatic effect, low price, good stability, soft and easy to deform, no adverse reactions, etc. There are many kinds of existing hemostatic dressings, such as zeolite powder, fibrin dressings, chitosan bandages and the like. Among them, chitosan is considered to be one of the most promising hemostatic dressings due to its excellent biocompatibility, hemostatic properties, and antibacterial properties. Existing chitosan-based hemostatic dressings are mainly chitosan acid salts (acetate or lactate, etc.), which have been commercialized and used in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/28A61L15/44A61L15/42
Inventor 胡巧玲黄晓飞王征科孙永福
Owner ZHEJIANG UNIV
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