Preparation method and use of oyster bioactive peptides

A technology of active peptides and oysters, applied in diseases, hydrolyzed protein components, fermentation, etc., can solve problems such as ignoring the unique effects of other components
CN104031964AActive Publication Date: 2014-09-10ZHEJIANG GONGSHANG UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG GONGSHANG UNIVERSITY
Publication Date
2014-09-10

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Abstract

The invention provides a preparation method of oyster bioactive peptides. The method comprises the following steps: carrying out high speed homogenate on fresh oyster meat, centrifuging, and adjusting the pH to 7 in order to obtain crude oyster proteins; centrifuging the obtained oyster protein suspension, homogenizing through a high speed disperser, adding Papain, maintaining the pH value of the obtained solution unchanged, heating reactants for enzyme killing, and cooling to obtain freeze-dried oyster protein powder; and preparing a solution, stirring, centrifuging, determining the content of amino acids in the above obtained supernatant which is a high temperature enzyme-killed oyster enzymatic hydrolysate through a biosensor, separating the oyster enzymatic hydrolysate through a SephadexG-25 chromatography column, eluting, freeze-drying, and carrying out gel column desalination to obtain the oyster bioactive peptides. The oyster enzymatic hydrolysate obtained in the invention contains high content of total amino acids, so the activity of biological components is effectively protected, and the ovarian granulosa cell function interference effect caused by bisphenol A can be substantially antagonized; and the oyster bioactive peptides can be applied in the preparation of medicines for treating the ovarian function disorder caused by the exposure of chemical environment interferent bisphenol A.
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Description

technical field

[0001] The invention belongs to the technical field of marine natural products and medicines, and relates to a preparation method of oyster active peptide and its application in the preparation of medicines for treating ovarian dysfunction caused by exposure to bisphenol A, a chemical environmental interferent. Background technique

[0002] Seafood oysters have great edible value and medicinal value, and are the largest cultured shellfish in the world, and are cultivated on a large scale in coastal areas such as Zhejiang Province in my country. The Ninghai area of ​​Zhejiang Province is known as the "Hometown of Oysters" and has a history of 700 years of oyster farming. The nutritional value of oysters is extremely high, the protein content in oysters is as high as 45%-57%, and its amino acid composition is perfect. According to the evaluation of the World Food and Agriculture Organization, the completeness and quality ratio of essential amino acids in oyste...

Claims

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