Jellyfish paraffin section making method

A technology for paraffin sectioning and jellyfish, which is applied in the field of preparation of paraffin sections, can solve the problems of difficult frozen sectioning, short time consumption, and few steps, and achieve the effects of shortening preparation time, reducing deformation, and good results

Active Publication Date: 2014-09-10
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Frozen sectioning technology has relatively few steps, short time consumption, and few reagents used, but sectioned samples are not suitable for long-term storage, especially the salted jellyfish raw material has a high salt content, and it is more difficult to freeze sectioned due to the influence of salt
Although the paraffin sectioning technique takes a long time and involves many steps, the embedded tissue blocks can be stored for a long time, and can be sectioned repeatedly for observation, unlike frozen sections that must be cut within a specified time, and it is suitable for most samples

Method used

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  • Jellyfish paraffin section making method
  • Jellyfish paraffin section making method
  • Jellyfish paraffin section making method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Material collection and fixation

[0062] Salvage the fresh jellyfish, divide it immediately, wash away the mucus and other impurities on the umbrella body, cut it into 4×4cm tissue pieces, quickly put it in neutral buffered formaldehyde fixative solution at 4°C for 10 hours, take it out and trim it to 0.5×0.5×0.5 cm tissue block, put into the embedding box, alcohol acetate formaldehyde mixed with fixative solution and fixed at 4°C for 12h.

[0063] 2. Dehydration and transparency

[0064] After fixation, the tissue was taken out of the fixative solution, and placed in different concentrations of ethanol and xylene solutions for gradient dehydration and transparency. The specific steps are as follows:

[0065] 80% ethanol 3h→90% ethanol 1.5h→95% ethanol 1h→100% ethanol 45min→100% ethanol 45min→1 / 3 xylene 30min→1 / 2 xylene 40min→full xylene 30min→full xylene 30min.

[0066] 3. Wax penetration and embedding:

[0067] After step 2, the completely dehydrated and transp...

Embodiment 2

[0076] 1. Material collection and fixation

[0077] Take out the salted jellyfish umbrella from the salting pool, wash the salt on the surface of the umbrella body with clean water, cut it into 1.5×4cm strips, fix it in neutral buffered formaldehyde fixative solution at room temperature for 8 hours, take out the trimmed pieces 0.5×0.5×0.5cm and put them in the bag Embed the box, fix with ethanol-acetate-formaldehyde fixative at 4°C for 8h.

[0078] 2. Dehydration and transparency

[0079] After fixation, the tissue was taken out of the fixative solution, and placed in different concentrations of ethanol and xylene solutions for gradient dehydration and transparency. The specific process is as follows:

[0080] 80% ethanol 2h→90% ethanol 1.5h→95% ethanol 1h→100% ethanol 30min→100% ethanol 30min→1 / 3 xylene 30min→1 / 2 xylene 40min→full xylene 30min→full xylene 30min.

[0081] 3. Wax penetration and embedding:

[0082] Put the tissue block treated in step 2 into 1:1 xylene:paraf...

Embodiment 3

[0090] 1. Material collection and fixation

[0091]Cut the salted jellyfish umbrella into 1.5×4cm long strips, the ratio of solid to liquid is 1:10, soak in clean water for 6 hours, change the water every 2 hours, put in neutral buffered formaldehyde fixative solution, fix at room temperature for 8 hours, take out and trim 0.5×0.5 ×0.5cm was put into alcohol-acetic acid-formaldehyde mixed fixative at room temperature and then fixed for 8h.

[0092] 2. Dehydration and transparency

[0093] After fixing the tissue, perform gradient dehydration and transparency, and the specific steps are the same as step 2 of Example 1.

[0094] 3. Wax penetration and embedding:

[0095] The tissue block treated in step 2 was immersed in 1:1 xylene: paraffin for 40 minutes, 58-60°C full paraffin for 30 minutes twice, took out the embedding box, wrapped in newspaper and cooled at 4°C for 30 minutes. Take out the cooled wax block, and embedding normally with LECIA RM2245 embedding machine, and ...

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Abstract

The invention discloses a jellyfish paraffin section making method. The method comprises the steps of raw materials processing and fixing, dehydration, transparency realization, waxing, embedding, sectioning and dyeing. The method is suitable for the fixing, dehydration, transparency realization, waxing, embedding, sectioning and dyeing of different raw jellyfishes. A secondary fixing method combining a neutral buffering formaldehyde fixing liquid with an alcohol-acetic acid-formaldehyde mixed liquid, and the formula of a routine alcohol-acetic acid-formaldehyde mixed fixing liquid is adjusted, so the dehydration time is shortened, and the section production efficiency is improved; and the waxing time and temperature control conditions suitable for the jellyfish are established. The tissue deformation problem in the jellyfish paraffin section making process is overcome in the invention, and ideal paraffin tissue blocks and dyeing effect are realized. The making is suitable for making jellyfish paraffin sections with high content and rich in collagens, and the method provides a histomorphologic support for the monitoring of the quality of jellyfish in the processing process.

Description

technical field [0001] The invention relates to a method for preparing paraffin slices, in particular to a method suitable for paraffin slices of various jellyfish raw materials. Background technique [0002] Jellyfish is a large edible jellyfish belonging to the invertebrate coelenterate phylum. The recorded jellyfish genera in the world include jellyfish, macular jellyfish, club jellyfish and warty jellyfish. [0003] Jellyfish is rich in nutrition, and its dry base contains high protein, calcium, phosphorus, iodine, iron and other inorganic salts and vitamins and other nutrients. In addition to eating jellyfish in China, jellyfish food is also popular in Japan, Thailand and other East and Southeast Asian regions. . Jellyfish is a traditional fishing target in my country's fishery. my country's jellyfish has good quality and high output. In 2012, the national jellyfish farming output was 63,790 tons, and the marine fishing volume was 206,885 tons. It ranks first in the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/36G01N1/30
Inventor 董秀萍马璐璐梁爽吴琼傅新鑫朱蓓薇范馨茹詹佳新
Owner DALIAN POLYTECHNIC UNIVERSITY
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