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A method for simultaneously extracting and separating tobacco chlorogenic acid, solanesol, alkaloids and rutin

A technology for solanesol and rutin, which is applied in the preparation of sugar derivatives, sugar derivatives, and sugar derivatives, can solve the problems of unsatisfactory extraction process, low extraction efficiency, and high extraction temperature, so as to reduce energy consumption and improve Utilization, efficient extraction effect

Active Publication Date: 2016-04-13
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the research and utilization of tobacco functional components are mainly based on the extraction and utilization technology of a single functional component. Although the comprehensive extraction technology of different functional components has begun to receive attention, it is mainly the comprehensive extraction technology of solanesol and nicotine. There are few reports on the comprehensive extraction technology of substances. Ai Xinxin of Jiangnan University reported the technology of simultaneously extracting tobacco solanesol, chlorogenic acid, and alkaloids with 95% ethanol ultrasonic assistance, and the extraction rates were 82% and 94% respectively. , 95%, the extraction efficiency of the three substances, especially solanesol, is low, and the extraction temperature required for the test is as high as 80°C, and the energy consumption of the test is high
[0004] It can be seen that most of the current technologies are single or a few extractions, and the extraction process is not ideal or the extraction rate is not high. At present, the comprehensive extraction of chlorogenic acid, solanesol, nicotine, and rutin from tobacco is efficiently extracted at the same time, and the realization of Solanesol, chlorogenic acid, rutin, and nicotine are effectively separated and the operation is simple, solvent-saving, and energy-saving technologies have not been reported yet

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  • A method for simultaneously extracting and separating tobacco chlorogenic acid, solanesol, alkaloids and rutin
  • A method for simultaneously extracting and separating tobacco chlorogenic acid, solanesol, alkaloids and rutin

Examples

Experimental program
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Embodiment 1

[0021] Such as figure 1 A method for simultaneously extracting and separating tobacco chlorogenic acid, solanesol, nicotine, and rutin is shown, and its steps include:

[0022] 1. Simultaneous extraction of tobacco chlorogenic acid, solanesol, nicotine and rutin

[0023] Weigh 500g of pulverized tobacco powder sample, extract once with 80% ethanol at the ratio of solid to liquid 1:10, ultrasonic frequency 45KHz, extraction temperature 40-50°C, and the residue is then extracted with 80% and 95% ethanol respectively. Extract once for 20 minutes; the obtained extracts are combined to obtain extracts of tobacco chlorogenic acid, solanesol, nicotine and rutin, and the extraction rates of the four components are all up to 100%.

[0024] 2. Separation of tobacco chlorogenic acid, solanesol, nicotine and rutin

[0025] 1. Separation of solanesol

[0026] The extract obtained in step 1 was concentrated under reduced pressure at 50°C until there was no alcohol smell, and the ethanol ...

Embodiment 2

[0033] Such as figure 2 As shown, the difference from Example 1 is:

[0034] 2. Separation of tobacco chlorogenic acid, solanesol, nicotine and rutin

[0035] 1. Separation of solanesol

[0036] The extract was concentrated under reduced pressure at 50°C until there was no alcohol smell, and ethanol was recovered. After the concentrated solution is cooled, it is quickly filtered, solanesol remains in the precipitate, and chlorogenic acid, rutin, and nicotine remain in the filtrate. Wash the precipitate with 20-40% ethanol solution, dissolve it in absolute ethanol, add 30% sodium hydroxide solution, saponify at 40-50°C under ultrasonic conditions for 40min, add deionized water after saponification, and then extract with petroleum ether 3 times, the extracts were combined and concentrated to obtain crude solanesol. The crude solanesol is refined by column chromatography, preparative chromatography, crystallization and other methods to obtain pure solanesol.

[0037] 2. Sep...

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Abstract

The invention relates to a method for simultaneously extracting and separating tobacco chlorogenic acid, solanesol, nicotine, and rutin. The steps include: Step 1. Simultaneous extraction: pulverizing tobacco raw materials at a temperature of 40-50°C and an ultrasonic frequency of 45KHz , under the condition of solid-liquid ratio 10:1, extract once with 80% ethanol, and after suction filtration or centrifugation, the residue is extracted once with 80% and 95% ethanol respectively; the three extracts are combined to obtain tobacco chlorogenic acid, solanum The crude extract of nicotinol, nicotine, and rutin, the extraction rates of tobacco chlorogenic acid, solanesol, nicotine, and rutin all reach 100%; Step 2, separation: separate solanesol; separate chlorogenic acid, Rutin; isolated nicotine. The present invention realizes simultaneous efficient extraction of tobacco chlorogenic acid, solanesol, nicotine, and rutin, and the extraction rate reaches 100%. The four are effectively separated through simple steps, and the utilization rate of tobacco resources is improved while the operation is simple. Save organic solvents and reduce energy consumption.

Description

technical field [0001] The invention relates to a comprehensive extraction and separation technology of tobacco functional components, in particular to a method for simultaneous extraction and separation of tobacco chlorogenic acid, solanesol, nicotine and rutin. Background technique [0002] The medicinal value of tobacco has been discussed in Chinese herbal medicine and traditional Chinese medicine literature. Modern analytical chemistry has proved that tobacco is rich in a variety of biologically active functional components. Tobacco is the most abundant plant solanesol (1-4%) resource; the content of chlorogenic acid (0.2-5.4%) in tobacco leaves is equivalent to that of honeysuckle; The functional components are comparable; nicotine and its related substances in tobacco are very promising as future drug candidates for the treatment of Alzheimer's disease. Nicotine pesticide is a traditional "green pesticide" with high efficiency and low toxicity. Tobacco also contains ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732C07C67/48C07C33/02C07C29/76C07D401/04C07H17/07C07H1/08
CPCC07C29/76C07C67/48C07D401/04C07H1/08C07H17/07C07C69/732C07C33/02
Inventor 杜咏梅张怀宝李晓芹张忠锋侯小东付秋娟王爱华
Owner TOBACCO RES INST CHIN AGRI SCI ACAD