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Preparation method of ginko biloba extract

A technology of ginkgo biloba leaves and extracts, applied in the field of preparation of ginkgo biloba extracts, can solve the problems of writhing reaction in mice, easy leakage of filtration, tissue necrosis, etc. The effect of content

Active Publication Date: 2014-10-15
SHINEWAY PHARMA GRP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN201110067189.2 Ginkgo leaves are extracted by water or alcohol, filtered with diatomaceous earth, and separated by a macroporous resin column to reduce the amount of burning residue in the extract of ginkgo leaves. Diatomaceous earth may also bring in other unknown impurities, which will bring potential harm to the preparation
[0010] Zhejiang Kangenbei Pharmaceutical Co., Ltd. has conducted research on the content of proanthocyanidins in Ginkgo biloba extract, and proposed an industry standard that the content of proanthocyanidins in Ginkgo biloba extract should not be less than 4%, but its preparations only involve oral preparations and do not involve injection
Lei Jieqiong et al observed that after intraperitoneal injection of proanthocyanidin 40mg / kg in mice, they found that the activity of the mice decreased, curled up, some mice showed writhing reactions, and 24 hours after intramuscular injection, there were exudate, hyperemia, tissue necrosis and other reactions at the administration site , which shows that the injection of proanthocyanidins has a great irritant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Weigh 1 kg of ginkgo biloba leaves produced in Jiangsu, coarsely crush them, add 8 times the amount of 70% ethanol solution, soak and extract at 60°C for 4 hours, extract 3 times, filter to obtain the extract, concentrate in vacuo (-0.07~-0.09MPa, 60°C) to the volume of the medicinal materials, then add purified water that is 4 times the amount of medicinal materials, refrigerate and stand at 0-10°C for 24 hours, extract the supernatant and filter, put the filtrate on a HPD-500 macroporous resin column, and wash with 1L of purified water Remove impurities, then elute with 3L of 75% ethanol, collect the eluate, concentrate in vacuum until there is no alcohol smell, add purified water twice the amount of medicinal materials, boil for 5 minutes, cool to room temperature and let stand for 12 hours, filter, and put the filtrate on the For the second HPD-500 macroporous resin column, use 1L of purified water to elute the impurities, then use 1L of 10% ethanol to elute the impu...

Embodiment 2

[0043] Weigh 1 kg of Ginkgo biloba leaves from Chongqing, coarsely crush them, add 6 times the amount of 80% ethanol solution, soak and extract at 65°C for 2 hours, extract 4 times, filter to obtain the extract, concentrate in vacuo (-0.07~-0.09MPa, 65°C) to 0.5 times the volume of medicinal materials, then add purified water 6 times the amount of medicinal materials, refrigerate and stand at 0-10°C for 48 hours, extract the supernatant and filter, put the filtrate on AB-8 macroporous resin column, use 1.5L Purified water was used to elute impurities, and then 4L of 70% ethanol was used to elute, and the eluate was collected, concentrated in vacuum until there was no alcohol smell, added purified water with 4 times the amount of medicinal materials, boiled for 10 minutes, cooled to room temperature and then stood for 24 hours, filtered , put the filtrate on the second AB-8 macroporous resin column, use 1.5L of purified water to elute the impurities, then use 1L15% ethanol to el...

Embodiment 3

[0045] Weigh 1kg of Ginkgo biloba leaves produced in Chongqing, coarsely crush them, add 10 times the amount of 60% methanol solution, soak and extract at 50°C for 3 hours, extract 3 times, filter to obtain the extract, concentrate in vacuo (-0.07~-0.09MPa, 50°C) to 2 times the volume of medicinal materials, then add purified water with 4 times the amount of medicinal materials, refrigerate and stand at 0-10°C for 36 hours, extract the supernatant and filter, put the filtrate on HPD-500 macroporous resin column, and use 1L to purify Elute the impurities with water, then elute with 3L of 75% methanol, collect the eluate, concentrate in vacuum until there is no alcohol smell, add purified water with 3 times the amount of medicinal materials, boil for 1 minute, cool to room temperature, let stand for 24 hours, filter, Put the filtrate on the second AB-8 macroporous resin column, elute the impurities with 1L of purified water, then elute the impurities with 1L of 25% methanol, and ...

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Abstract

The invention provides a preparation method of a ginko biloba extract. According to the method, ginko biloba leaves are used as raw materials, and the ginko biloba extract is prepared through extraction with alcohol or acetone, water precipitation, purification by a macroporous resin column, boiling water precipitation, secondary purification by the macroporous resin column, refrigerating precipitation and alcohol or acetone low-temperature water-soluble refining. The extract has the characteristics of water solubility, low phenolic acid, low anthocyanidi and low residue on ignition; in the ginko biloba extract contains at least 24% of the total flavonoid glycosides, at least 9.5% of terpene lactones, no more than 0.3% of residues on ignition, no more than 0.3% of proanthocyanidins and no more than 1 ppm of total ginkgolic acid, and the cold water solubility is not lower than 5%. According to the method, the quality of the ginko biloba extract is improved with a two-step resin column method and a two-step precipitation method, and the method is simple in technological operation, good in phenolic acid removal effect, stable in technology and applicable to industrial production.

Description

technical field [0001] The invention relates to a preparation method of ginkgo leaf extract, which belongs to the technical field of extraction and separation of natural plant effective components. Background technique [0002] Ginkgo biloba extract (Ginko Biloba Extract, EGB) is the leaf extract of Ginkgo biloba L. It can effectively scavenge free radicals, reduce the cytotoxicity of NO induced by β-amyloid protein (Aβ), protect nerve cells, improve cognitive functions such as attention and short-term memory, and is symmetrical to Alzheimer's disease, aging, and neuropathy. , learning, and memory functions are all improved. Ginkgo biloba preparations are currently mainly used clinically for middle-aged and elderly brain insufficiency and senile dementia caused by coronary heart disease, angina pectoris, myocardial infarction, stroke, cerebral circulation disorder and brain function reversion. [0003] EGB mainly contains total flavonol glycosides, terpene lactones, proant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P25/28A61P39/06A61K127/00
Inventor 冯玉康孙胜斌姜国志刘铁军张岩岩霍宝军陈钟李振江
Owner SHINEWAY PHARMA GRP LTD
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