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Mycorrhizal fungi inoculant and application of mycorrhizal fungi inoculant in degradation of residual tetracycline antibiotics

A technology of mycorrhizal fungi and tetracyclines, applied in the field of contaminated soil remediation, to achieve the effects of increasing the contact area, simple operation, and reducing residues

Active Publication Date: 2014-10-15
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports about the effects of arbuscular mycorrhizal fungi on the degradation of tetracycline antibiotics in soil and the impact of plant uptake

Method used

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  • Mycorrhizal fungi inoculant and application of mycorrhizal fungi inoculant in degradation of residual tetracycline antibiotics
  • Mycorrhizal fungi inoculant and application of mycorrhizal fungi inoculant in degradation of residual tetracycline antibiotics
  • Mycorrhizal fungi inoculant and application of mycorrhizal fungi inoculant in degradation of residual tetracycline antibiotics

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 mycorrhizal fungus inoculum

[0030] The arbuscular mycorrhizal agent used in the present invention is that the mycorrhizal fungal agent is obtained by multiplying the bacterial species inoculant in the greenhouse, wherein the bacterial species inoculant (Glomus lanus (XJ03C), Moses Glomus (NM03D) and Glomus contortus (NM03A)) were purchased from the "China Arbuscular Mycorrhizal Fungi Germplasm Resource Bank", and the specific preparation method of the bacterial agent is as follows:

[0031] The multiplication culture is carried out in the greenhouse. The conditions in the greenhouse are: carried out in a growth room with controlled environment, with about 16 hours of daytime light and a temperature of about 25°C, and about 8 hours of nighttime light with a temperature of about 18°C. Osram fluorescent lamps are used for both day and night Lighting, the light intensity is 550μEm -2 the s -1 about. Put the sterilized culture medium (zeo...

Embodiment 2

[0032] Example 2 Degradation effect experiment of Glomus tertiotomycin with different initial concentrations of oxytetracycline in oxytetracycline-contaminated soil

[0033] The initial content of oxytetracycline is 1.0kg of oxytetracycline-contaminated soil of 1mg / kg, 10mg / kg, 50mg / kg and is respectively packed in different flower pots, and is mixed into the young capsular mycotic agent 30g that embodiment 1 prepares respectively At the same time, a control treatment without inoculation of any bacterial agent is set, three parallel samples are set for each treatment in the four treatment modes, the soil water content is adjusted to be 60% of the maximum field water holding capacity, and the soil is left to stand overnight, and the alfalfa or corn seeds are treated with 10wt %H 2 o 2After disinfection and germination, sow in the soil that has been inoculated with young capsule fungal agent. Sow 3 seeds per pot of corn, 9 seeds per pot of alfalfa, and sow them in different pot...

Embodiment 3

[0035] Example 3 Degradation effect experiment of Glomus mosei on different initial concentrations of oxytetracycline in oxytetracycline-contaminated soil

[0036] The oxytetracycline contaminated soil 1.0kg that the oxytetracycline initial content is 1mg / kg, 10mg / kg, 50mg / kg is packed in different flower pots respectively, and mixes respectively the Moxi balloon mold agent 30g that embodiment 1 prepares, Set up the control treatment that does not inoculate any bacterial agent at the same time, set 3 parallel samples for each treatment in four kinds of treatment modes, adjust soil water content to be 60% of maximum field water holding capacity, stand overnight, alfalfa or corn seed with 10wt% h 2 o 2 After disinfection and germination, sow the seeds in the soil that has been inoculated with the moxie glomus fungal agent. Sow 3 seeds per pot of corn and 9 seeds per pot of alfalfa. 1 corn, 7 alfalfa. Then manage according to the management mode of common crops, and after 90 d...

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Abstract

The invention discloses a mycorrhizal fungi inoculant and an application of the mycorrhizal fungi inoculant in degradation of residual tetracycline antibiotics. The inoculant comprises a culture medium and spores and / or mycelia of arbuscular mycorrhizal fungi mixed in the culture medium. The application of the mycorrhizal fungi inoculant in the degradation of the residual tetracycline antibiotics specially comprises the processes as follows: the mycorrhizal fungi inoculant is inoculated in soil polluted by the tetracycline antibiotics, and then a host plant is sowed in the soil, so that a soil remediation system polluted by the tetracycline antibiotics is established. The host plant is harvested after growing for 90-120 days, then the concentration of the tetracycline antibiotics in the soil is measured, and the degradation rate can reach 20%-60%. The method provides the basis for arbuscular mycorrhizal fungi used for degradation of high-concentration tetracycline antibiotics residues in the soil.

Description

technical field [0001] The invention relates to the field of contaminated soil remediation, in particular to a mycorrhizal fungal agent and its application in degrading residual tetracycline antibiotics. Background technique [0002] Antibiotics used in large quantities by humans and livestock will eventually enter the environment and become new pollutants and cutting-edge research topics. In particular, the use of livestock and poultry waste as organic fertilizer in agricultural production may cause soil antibiotic pollution and endanger the safety of agricultural products. According to statistics, the annual consumption of antibiotics in the animal breeding industry in the United States is about 11,000 tons, accounting for about 70% of the total antibiotic consumption; the annual consumption of antibiotics in the animal breeding industry in the European Community has reached more than 5,000 tons. In my country, about tens of thousands of tons of antibiotics are used in li...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/645
Inventor 陈莉张乙涵
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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