Mycorrhizal fungi inoculant and application of mycorrhizal fungi inoculant in degradation of residual tetracycline antibiotics

A technology of mycorrhizal fungi and tetracyclines, applied in the field of contaminated soil remediation, to achieve the effects of increasing the contact area, simple operation, and reducing residues

Active Publication Date: 2014-10-15
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports about the effects of arbuscular mycorrhizal fungi on

Method used

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  • Mycorrhizal fungi inoculant and application of mycorrhizal fungi inoculant in degradation of residual tetracycline antibiotics
  • Mycorrhizal fungi inoculant and application of mycorrhizal fungi inoculant in degradation of residual tetracycline antibiotics
  • Mycorrhizal fungi inoculant and application of mycorrhizal fungi inoculant in degradation of residual tetracycline antibiotics

Examples

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Example Embodiment

[0029] Example 1 Preparation of mycorrhizal fungal inoculum

[0030] The arbuscular mycorrhizal inoculum used in the present invention, that is, the mycorrhizal fungal inoculum, is prepared by multiplying the bacterial inoculant in the greenhouse, wherein the bacterial inoculant (XJ03C), Moses Glomus (NM03D) and Glomus twist (NM03A)) were purchased from the "Chinese Arbuscular Mycorrhizal Fungi Germplasm Library". The specific preparation method of the inoculum is as follows:

[0031] Multiplication and cultivation are carried out in a greenhouse. The conditions in the greenhouse are: in a growth room with controlled environment, daytime illumination is about 16h, temperature is about 25℃, night time is about 8h, temperature is about 18℃, and Osram fluorescent lamps are used for both day and night. Illumination, the light intensity is 550μEm -2 s -1 about. Put the sterilized culture medium (zeolite: river sand=1:1, volume ratio) into 2 / 3 of the plastic basin, spread the bacterial ...

Example Embodiment

[0032] Example 2 Degradation effect experiment of Glomus juveniles on oxytetracycline with different initial concentrations in soil contaminated with oxytetracycline

[0033] Put 1.0 kg of oxytetracycline-contaminated soil with initial oxytetracycline content of 1mg / kg, 10mg / kg, 50mg / kg in different pots, and mix them with 30g of the young mantle saccharomycete prepared in Example 1. At the same time, a control treatment without inoculation of any bacterial agents was set up. In each of the four treatments, three parallel samples were set up. Adjust the soil moisture content to 60% of the maximum field water holding capacity. Leave it to stand overnight. Use 10wt. alfalfa or corn seeds. %H 2 O 2 After disinfection and germination, they were sown in the soil that had been inoculated with the saccharomycetes. 3 corn per pot and 9 alfalfa per pot were sown in different pots. The seedlings were thinned 3 days after emergence and left in each pot. 2 corn plants and 7 alfalfa plants; t...

Example Embodiment

[0035] Example 3 Degradation effect experiment of Glomus mosesii on different initial concentrations of oxytetracycline in soil contaminated with oxytetracycline

[0036] Put 1.0 kg of oxytetracycline contaminated soil with initial oxytetracycline content of 1 mg / kg, 10 mg / kg, and 50 mg / kg in different pots, and mix them with 30 g of the Moses sacculus prepared in Example 1. At the same time, a control treatment without inoculation of any bacterial agents was set. In each of the four treatments, 3 parallel samples were set for each treatment. Adjust the soil water content to 60% of the maximum field water holding capacity. Leave it to stand overnight and use 10wt% of alfalfa or corn seeds. H 2 O 2 After disinfection and sprouting, sown in the soil that has been inoculated with Moses globulin. 3 corn per pot and 9 alfalfa per pot. Sown in different pots. Thinning is carried out 3 days after emergence, leaving 2 per pot. Plant corn, 7 alfalfa. Then, it is managed according to the ...

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Abstract

The invention discloses a mycorrhizal fungi inoculant and an application of the mycorrhizal fungi inoculant in degradation of residual tetracycline antibiotics. The inoculant comprises a culture medium and spores and/or mycelia of arbuscular mycorrhizal fungi mixed in the culture medium. The application of the mycorrhizal fungi inoculant in the degradation of the residual tetracycline antibiotics specially comprises the processes as follows: the mycorrhizal fungi inoculant is inoculated in soil polluted by the tetracycline antibiotics, and then a host plant is sowed in the soil, so that a soil remediation system polluted by the tetracycline antibiotics is established. The host plant is harvested after growing for 90-120 days, then the concentration of the tetracycline antibiotics in the soil is measured, and the degradation rate can reach 20%-60%. The method provides the basis for arbuscular mycorrhizal fungi used for degradation of high-concentration tetracycline antibiotics residues in the soil.

Description

technical field [0001] The invention relates to the field of contaminated soil remediation, in particular to a mycorrhizal fungal agent and its application in degrading residual tetracycline antibiotics. Background technique [0002] Antibiotics used in large quantities by humans and livestock will eventually enter the environment and become new pollutants and cutting-edge research topics. In particular, the use of livestock and poultry waste as organic fertilizer in agricultural production may cause soil antibiotic pollution and endanger the safety of agricultural products. According to statistics, the annual consumption of antibiotics in the animal breeding industry in the United States is about 11,000 tons, accounting for about 70% of the total antibiotic consumption; the annual consumption of antibiotics in the animal breeding industry in the European Community has reached more than 5,000 tons. In my country, about tens of thousands of tons of antibiotics are used in li...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/645
Inventor 陈莉张乙涵
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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