Check patentability & draft patents in minutes with Patsnap Eureka AI!

Functionalized nano hydroxyapatite gene delivery material, preparation method, and applications thereof

A nano-hydroxyapatite and hydroxyapatite technology, which is applied in gene therapy, pharmaceutical formulations, and the introduction of foreign genetic material using a carrier, can solve the problem of high toxicity of cationic liposomes, difficult to obtain raw materials for preparation, and gene gun penetration. problems such as limited permeability, to achieve good biocompatibility, reduced activity, and good dispersibility

Inactive Publication Date: 2014-10-22
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the non-viral gene transfer method is a current research focus, but the above-mentioned non-viral gene transfer methods all have shortcomings: microinjection can only process one cell at a time, and its transfection efficiency is very low; Penetration is very limited; the transfection efficiency of the calcium phosphate co-precipitation method is affected by many factors such as temperature, concentration, and operating environment, and the transfection result is very unstable; although the cationic liposome method shows good transfection efficiency, the cationic liposome method Due to the high toxicity of liposomes, the application of the cationic liposome method is limited; the nano-gene transfer materials in the prior art have the disadvantages of high production cost, difficult availability of raw materials for preparation, and difficulty in popularization and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Functionalized nano hydroxyapatite gene delivery material, preparation method, and applications thereof
  • Functionalized nano hydroxyapatite gene delivery material, preparation method, and applications thereof
  • Functionalized nano hydroxyapatite gene delivery material, preparation method, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for preparing a gene transfer material of functionalized nano-hydroxyapatite, comprising the following steps:

[0038] Step 1, preparation of needle-shaped hydroxyapatite: Slowly add an aqueous solution of calcium hydroxide to an aqueous solution of dilute phosphoric acid, and stir at a temperature of 37° C. for 24 hours, and then filter after standing for 2 hours to obtain particles. Then the particles were washed with water and dried in vacuum to obtain needle-shaped hydroxyapatite.

[0039] Wherein, in step 1, the molar ratio of calcium hydroxide in the aqueous solution of calcium hydroxide to phosphoric acid in the aqueous solution of dilute phosphoric acid is 5:3. Wherein, in the aqueous solution of calcium hydroxide, the molar concentration of calcium hydroxide is 0.17mol / L. In the aqueous solution of dilute phosphoric acid, the molar concentration of dilute phosphoric acid is 0.15mol / L.

[0040] Step 2, adsorption of cisplatin: Dissolve cis-diaminodich...

Embodiment 2

[0045] A method for preparing a gene transfer material of functionalized nano-hydroxyapatite, comprising the following steps:

[0046] Step 1, preparation of needle-shaped hydroxyapatite: Slowly add an aqueous solution of calcium hydroxide to an aqueous solution of dilute phosphoric acid, and stir at a temperature of 33° C. for 26 hours, and then filter after standing for 1.5 hours to obtain particles. Then the particles were washed with water and dried in vacuum to obtain needle-shaped hydroxyapatite.

[0047]Wherein, in step 1, the molar ratio of calcium hydroxide in the aqueous solution of calcium hydroxide to phosphoric acid in the aqueous solution of dilute phosphoric acid is 4:2. Wherein, in the aqueous solution of calcium hydroxide, the molar concentration of calcium hydroxide is 0.1mol / L. In the aqueous solution of dilute phosphoric acid, the molar concentration of dilute phosphoric acid is 0.1mol / L.

[0048] Step 2, adsorption of cisplatin: dissolving cis-diaminodic...

Embodiment 3

[0053] A method for preparing a gene transfer material of functionalized nano-hydroxyapatite, comprising the following steps:

[0054] Step 1, preparation of needle-shaped hydroxyapatite: Slowly add an aqueous solution of calcium hydroxide to an aqueous solution of dilute phosphoric acid, and stir at a temperature of 40° C. for 22 hours, and then filter after standing for 2.5 hours to obtain particles. Then the particles were washed with water and dried in vacuum to obtain needle-shaped hydroxyapatite.

[0055] Wherein, in step 1, the molar ratio of calcium hydroxide in the aqueous solution of calcium hydroxide to phosphoric acid in the aqueous solution of dilute phosphoric acid is 6:4. Wherein, in the aqueous solution of calcium hydroxide, the molar concentration of calcium hydroxide is 0.3mol / L. In the aqueous solution of dilute phosphoric acid, the molar concentration of dilute phosphoric acid is 0.3mol / L.

[0056] Step 2, adsorption of cisplatin: dissolving cis-diaminodi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of gene delivery materials, and specifically relates to a functionalized nano hydroxyapatite gene delivery material, a preparation method and applications thereof. The preparation method comprises the following steps: step one, preparing needle-shaped hydroxyapatite; and step two, making the hydroxyapatite absorb cis-platinum so as to obtain the nano hydroxyapatite gene delivery material. The prepared nano hydroxyapatite gene delivery material is a nano spherical material with an average diameter of around 100 nm, is capable of combining with green fluorescent protein plasmid gene pGFP to carry out transfection, and has the advantages of high transfection efficiency, good cell compatibility, and high cell survival rate. For instance, in the human breast cancer cells, the transfection efficiency of combined nano hydroxyapatite gene delivery material and pEGFP-C1 can reach 40% more or less.

Description

technical field [0001] The invention relates to the technical field of gene transfer materials, in particular to a functionalized nano-hydroxyapatite gene transfer material and its preparation method and application. Background technique [0002] Gene delivery methods can be divided into two categories: the first category is viral gene delivery methods, which use retroviruses, adenoviruses, and adeno-associated viruses as carriers; the second category is non-viral gene delivery methods, such as microinjection, gene Gun, calcium phosphate co-precipitation, cationic liposome method, and the use of emerging nano-gene delivery materials for gene transfection. [0003] Viral gene transfer methods have many serious disadvantages, such as the possibility of activation of proto-oncogenes during viral transfection. Therefore, the non-viral gene transfer method is a current research focus, but the above-mentioned non-viral gene transfer methods all have shortcomings: microinjection c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/63A61K48/00A61P35/00
Inventor 王深明林颖张德元常光其周鸿雁李梓伦邵楠
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com