Production of apple flavonoids by using tissue culture method

A tissue culture and flavonoid technology, applied in the field of biology and plant biology, can solve the problems of poor or poor quality of fresh food, low separation ratio, and difficulty in new functional apple varieties.

Active Publication Date: 2014-10-29
SHANDONG AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0004] In addition to the above-mentioned red-flesh and crispy-flesh strains with large fruit size, small fruit size (single fruit weight 20-40g), extremely low segregation ratio, and poor or very poor fresh food quality can also be bred from the hybrid F1 population. Poor, but high flavonoid

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  • Production of apple flavonoids by using tissue culture method
  • Production of apple flavonoids by using tissue culture method
  • Production of apple flavonoids by using tissue culture method

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Experimental program
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Embodiment 1

[0046] Embodiment 1, utilize tissue culture method to improve apple flavonoids

[0047] 1. Obtaining of explants

[0048] 1. Construct F1 segregation population

[0049] The 'Xiahongrou' Xinjiang red flesh apple preserved in the Xinjiang Tire National Germplasm Resource Garden was used as the male parent and the 'Red Fuji' apple variety was used as the female parent to construct a hybrid first generation (F1) segregation population;

[0050] 2. From F 1 Separation of populations for selection of explants

[0051] Primers were designed according to the red apple transcription factor MYB10 promoter sequence (R6),

[0052] The primer sequences are as follows:

[0053] F: 5'-GGTGGTCAAAGATGTGTGTTG-3' (SEQ ID NO: 1)

[0054] R: 5'-CTTATCTTTTGCCTGCTACCC-3' (SEQ ID NO: 2)

[0055] Using the genomic DNA of the apple pulp tissue of the hybrid first generation (F1) population as a template, the above primers were used for PCR amplification, and the band with a size of 504 bp was obta...

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Abstract

The invention discloses production of apple flavonoids by using a tissue culture method. The invention provides a method for preparing the flavonoids by using the tissue culture method, wherein the method comprises the following steps: 1) carrying out induction culture of an R6R6 genotype plant explant in a callus induction culture medium, to obtain a callus; 2) carrying out successive transfer culture of the callus in a successive transfer culture medium, to obtain a successive transfer callus; 3) carrying out enhancement culture of the successive transfer callus in an enhancement culture medium, to obtain an enhanced callus; and 4) extracting the flavonoids in the enhanced callus, to obtain the objective product. Experiments prove that the callus cultured by the method has the flavonoids content more than 30 times that of fruit pulp flavonoids of 'Jinshuai' and other apple varieties, and industrialized production of the apple flavonoids can be realized by using the tissue culture method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of plant biotechnology, in particular to the production of apple flavonoids by tissue culture. Background technique [0002] "The same origin of medicine and food" is the direction of development. Flavonoids are a kind of main polyphenols in apple fruit, which have various physiological functions on human health; while apple is a worldwide fruit, due to its strong ecological adaptability, high fruit nutritional value, good storability and supply The cycle is long, and many countries in the world have listed it as the main consumer fruit and strongly recommend it; my country is the world's largest producer and consumer of apples, of which 39.5 million tons of apples were produced in 2012, mainly for fresh food, and nearly 70% is the 'Red Fuji' variety which is low in flavonoids. Therefore, in order to ensure that consumers get enough polyphenols and flavonoids from apples, e...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 冀晓昊陈学森张宗营王楠姜生辉毛志泉吴树敬陈晓流张艳敏
Owner SHANDONG AGRICULTURAL UNIVERSITY
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