Hemoperfusion Devices for Experimental Animals

Abstract

The invention discloses a blood perfusion device for experimental animals. The device provided by the present invention comprises an arterial indwelling needle, a three-way valve, a constant flow pump, a perfuser, an exhaust tube, an infusion heater and an intravenous indwelling needle connected in sequence through a catheter. The invention uses rabbits as experimental animals, establishes vascular access via the central ear artery and the marginal ear vein through local anesthesia, simplifies the blood perfusion operation, relieves the pain of the experimental animals, and reduces the influence on the experimental results. The indwelling needle is soft, does not damage the inner wall of the blood vessel, can be retained for a long time, and is convenient for multiple blood perfusion. The arterial end is connected with a three-way valve to facilitate blood collection and injection of anticoagulant and other drugs. The internal volume of the perfusion device is 20ml. The reduction of blood volume in rabbits during hemoperfusion has no obvious impact on the basic vital signs. After the perfusion beam, the blood in the system is returned to the animal body through a constant flow pump to reduce blood loss. An infusion warmer is installed at the venous end to compensate for the loss of extracorporeal circulation blood temperature and maintain a constant body temperature of the experimental animals.

Description

technical field [0001] The invention belongs to the field of medical equipment and relates to a blood perfusion device for experimental animals. Background technique [0002] Hemoperfusion (HP) is one of the commonly used blood purification methods in clinical practice. It draws the patient's blood from the body through puncture and other methods for extracorporeal circulation, and uses the adsorption of the adsorbent in the extracorporeal circulation perfusion device to remove exogenous blood. and blood purification treatment of endogenous toxins. Hemoperfusion for poisoning treatment generally uses activated charcoal or resin as the adsorbent material. Activated carbon is composed of criss-cross voids, and a large number of micropores constitute a huge specific surface area. The solute reaches the surface of activated carbon particles through the liquid phase, and then is adsorbed in the voids. This adsorption is non-specific and has a certain Adsorption. The resin main...

AI Technical Summary

Problems solved by technology

Due to the complex genetics and microbial background of hybrid dogs; and the high cost of experimental Beagle dogs and harsh breeding conditions, it is not convenient to use dogs as experimental animals to carry out experiments

There are also hemoperfusion experiments using rabbits as experimental animals. After general anesthesia, hemoperfusion is performed through carotid artery / venous cannulation. Surgery is required to separate the large blood vessels in the neck. The trauma is relatively large, the postoperative survival time is short, and the experimental operation is complicated.

General anesthesia drugs may also interfere with poison metabolism and hemoperfusion effect, affecting the objective evaluation of hemoperfusion effect

In addition, surgical wounds are prone to infection and bleeding, which increases the pain and accidental death of animals, increases the amount of animals used, and is also contrary to animal ethics and the "3R" principle

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