Continuous flow method for measuring total alkaloid in tobacco or tobacco products

A technology for tobacco products and plant alkaloids, which is applied in the preparation of test samples, measurement of color/spectral characteristics, and material analysis by observing the impact on chemical indicators, etc. It can solve the problems of inconvenient use and large amount of chemicals

Active Publication Date: 2014-11-05
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In YC / T468-2013, there are also requirements for a suitable acid-base environment for the reaction. The acid-base environment of the reaction is also controlled by buffer solution A and buffer solution B. Although the acid-base environment of the reaction can be effectively controlled, the method buffer solution The amount of chemicals used in the preparation of A and buffer solution B is relatively large, which also brings inconvenience to the use of this method

Method used

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  • Continuous flow method for measuring total alkaloid in tobacco or tobacco products
  • Continuous flow method for measuring total alkaloid in tobacco or tobacco products
  • Continuous flow method for measuring total alkaloid in tobacco or tobacco products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1 : Determination of total plant alkaloids in flue-cured tobacco

[0080] 1) Sample pretreatment

[0081] Prepare the flue-cured tobacco powder into a sample according to YC / T31, and measure its moisture content, then weigh 0.25g of the sample in a 50mL Erlenmeyer flask with a stopper, add 25mL of 5% acetic acid, cover the stopper, and vibrate on a shaker (rotating speed >150rpm) extraction for 30min. Filter the extract with rapid qualitative filter paper, discard the first 2mL-3mL filtrate, and collect the subsequent filtrate for analysis.

[0082] 2) Preparation of reaction reagents

[0083] The preparation of buffer solution A is as follows: Weigh 71.6 grams of disodium hydrogen phosphate (12 crystal waters) and 15.2 grams of trisodium phosphate (12 crystal waters) into a beaker, dissolve them in water, then transfer them to a 1L volumetric flask, and dilute with water. To the scale.

[0084] The preparation of buffer solution B is: weigh 7g p-aminobenz...

Embodiment 2

[0109] Embodiment 2: the mensuration of total plant alkaloids in Burley tobacco

[0110] 1) Sample pretreatment

[0111] Prepare a sample of Burley tobacco powder according to YC / T31, and measure its moisture content, then weigh 0.25g of Burley tobacco sample in a 50mL conical flask with a stopper, add 25mL of 5% acetic acid, cover the stopper, and shake Shake on the device (rotational speed > 150rpm) and extract for 30min. Filter the extract with rapid qualitative filter paper, discard the first 2mL-3mL filtrate, and collect the subsequent filtrate for analysis.

[0112] 2) Preparation of reaction reagents

[0113] The preparation of buffer solution A is as follows: Weigh 71.6 grams of disodium hydrogen phosphate (12 crystal waters) and 15.2 grams of trisodium phosphate (12 crystal waters) into a beaker, dissolve them in water, then transfer them to a 1L volumetric flask, and dilute with water. To the scale.

[0114] The preparation of buffer solution B is: weigh 7g p-ami...

Embodiment 3

[0138] Embodiment 3: Determination of total plant alkaloids in cigarette

[0139] 1) Sample pretreatment

[0140] Prepare cigarette shredded tobacco as a sample according to YC / T31, and measure its moisture content, then weigh 0.25g of the cigarette sample into a 50mL Erlenmeyer flask with a stopper, add 25mL of 5% acetic acid, cover the stopper, and oscillate on a shaker (speed >150rpm) extraction for 30min. Filter the extract with rapid qualitative filter paper, discard the first 2mL-3mL filtrate, and collect the subsequent filtrate for analysis.

[0141] 2) Preparation of reaction reagents

[0142] The preparation of buffer solution A is as follows: Weigh 83.0 grams of disodium hydrogen phosphate (12 crystal waters) and 20.0 grams of trisodium phosphate (12 crystal waters) into a beaker, dissolve them in water, then transfer them to a 1L volumetric flask, and dilute with water. To the scale.

[0143] The preparation of buffer solution B is: weigh 7g of p-aminobenzenesul...

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Abstract

The invention provides a continuous flow method for measuring total alkaloid in tobacco or tobacco products. In the method, potassium thiocyanate and sodium dichloro isocyanurate are adopted to carry out reactions to generate cyanogen chloride on line. The cyanogen chloride can carry out reactions with total alkaloid (calculated in the form of nicotine) in tobacco or tobacco products to break off the pyridine rings of nicotine, and then further carries out reactions with p-aminobenzene sulfonic acid. The reaction products are measured by a chromometer at 460 nm. The method uses a buffer system to control the pH value of the reaction system in a range of 6.0 to 7.5, and the buffer system is composed of a buffer solution A and a buffer solution B. The buffer solution A is prepared by the following steps: weighing disodium hydrogen phosphate and trisodium phosphate, placing the weighed substances in a beaker, dissolving the substances with water, transferring the solution to a volumetric flask (1L), and adding water into the volumetric flask until the water reaches the scale. The buffer solution B is prepared by the following steps: weighing p-aminobenzene sulfonic acid, disodium hydrogen phosphate, sodium dihydrogen phosphate, and sodium citrate, placing the weighed substances into a beaker, dissolving the substances with water, transferring the solution to a volumetric flask (1L), and then adding water into the volumetric flask until the water reaches the scale.

Description

technical field [0001] The invention belongs to the technical field of tobacco chemical component detection, in particular to a method for measuring total alkaloids in tobacco or tobacco products by a continuous flow method. Background technique [0002] Nicotine is an important component of tobacco, and without nicotine, it cannot be called tobacco. In the routine analysis of tobacco, the content information of nicotine is usually obtained by measuring the content of total plant alkaloids in tobacco. The standards currently issued by the tobacco industry on the determination of total alkaloids in tobacco include: GB / T23225-2008 "Determination of total alkaloids in tobacco and tobacco products by photometry", YC / T160-2002 "Determination of total alkaloids in tobacco and tobacco products Continuous flow method" and YC / T468-2013 "Continuous flow (potassium thiocyanate) method for the determination of total plant alkaloids in tobacco and tobacco products". [0003] The contin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N1/28G01N21/31
Inventor 张威刘楠马雁军胡清源邢军侯宏卫罗安娜何声宝王英元冯晓民
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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