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Biosensor sensitive membrane and application in detection of clenbuterol hydrochloride

A clenbuterol hydrochloride and biosensor technology, applied in the field of biosensors, can solve problems such as difficult implementation of the method, and achieve the effects of easy analysis, reasonable design, and low detection limit

Active Publication Date: 2014-11-12
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a biosensor sensitive film, which solves the problem that the method of immobilizing antibodies on the chip surface of the biosensor to detect clenbuterol hydrochloride is difficult to realize in the prior art

Method used

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  • Biosensor sensitive membrane and application in detection of clenbuterol hydrochloride
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  • Biosensor sensitive membrane and application in detection of clenbuterol hydrochloride

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Embodiment 1

[0056] The biosensor sensitive membrane of the present embodiment, such as figure 1 shown, was prepared by the following method:

[0057] 1) Preparation of gold film: put a 2×2cm glass substrate in a vacuum evaporation machine, and use a vacuum pump to evacuate the vacuum to make the vacuum in the coating chamber reach 1.3×10 -2 Pa, heating the crucible to melt the high-purity gold wire at a temperature of 1400°C and evaporate it into gaseous gold. The gaseous gold particles are deposited on the surface of the moving glass substrate, and a continuous and bright layer is formed on the glass substrate after cooling and reduction. Gold film, the thickness of the gold film is 47-50nm;

[0058] 2) Pretreatment of the gold film loaded on the glass substrate: put the gold film loaded on the glass substrate into H 2 o 2 Soak in a mixed solution with a volume ratio of 3:7 to concentrated sulfuric acid (98% by mass) for 2 minutes, take it out, wash it with ethanol and deionized water...

Embodiment 2

[0082] The biosensor sensitive film of this embodiment is prepared by the following method:

[0083] 1) Preparation of gold film: put a 2×2cm glass substrate in a vacuum evaporation machine, and use a vacuum pump to evacuate the vacuum to make the vacuum in the coating chamber reach 1.3×10 -3 Pa, heating the crucible to melt the high-purity gold wire at a temperature of 1200°C and evaporate it into gaseous gold. The gaseous gold particles are deposited on the surface of the moving glass substrate, and a continuous and bright layer is formed on the glass substrate after cooling and reduction. Gold film, the thickness of the gold film is 47-50nm;

[0084] 2) Pretreatment of the gold film loaded on the glass substrate: put the gold film loaded on the glass substrate into H 2 o 2 Soak in a mixed solution with a volume ratio of 3:7 to concentrated sulfuric acid (mass concentration: 98%) for 4 minutes, take it out, wash it with ethanol and deionized water in turn, dry it for later...

Embodiment 3

[0108] The biosensor sensitive film of this embodiment is prepared by the following method:

[0109] 1) Preparation of gold film: put a 2×2cm glass substrate in a vacuum evaporation machine, and use a vacuum pump to evacuate the vacuum to make the vacuum in the coating chamber reach 1.3×10 -2 Pa, heating the crucible to melt the high-purity gold wire at a temperature of 1400°C and evaporate it into gaseous gold. The gaseous gold particles are deposited on the surface of the moving glass substrate, and a continuous and bright layer is formed on the glass substrate after cooling and reduction. Gold film, the thickness of the gold film is 47-50nm;

[0110] 2) Pretreatment of the gold film loaded on the glass substrate: put the gold film loaded on the glass substrate into H 2 o 2 Soak in a mixed solution with a volume ratio of 3:7 to concentrated sulfuric acid (98% by mass) for 2 minutes, take it out, wash it with ethanol and deionized water in turn, dry it for later use, and re...

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Abstract

The invention discloses a biosensor sensitive membrane and application in detection of clenbuterol hydrochloride. The sensitive membrane is prepared by the method of: 1) placing a gold membrane loaded on a glass substrate in an ethanol solution of n-Octadecyl mercaptan to perform soaking, and then conducting flushing and drying, thus obtaining a first self-assembled gold membrane; 2) putting the first self-assembled gold membrane in a coupling solution of amino-functionalized graphene or carboxyl-functionalized graphene oxide and goat anti-mouse IgG to perform soaking, then conducting flushing with a phosphate buffer solution and carrying out drying, thus obtaining a second self-assembled gold membrane; and 3) adding an antibody solution dropwise to the surface of the second self-assembled gold membrane to perform antibody assembling so as to make the antibody cover the surface of the second self-assembled gold membrane, conducting standing, then performing flushing with a phosphate buffer solution and carrying out drying. The biosensor sensitive membrane provided by the invention undergoes recognition combination with an antibody through a clenbuterol hydrochloride antigen and its conjugates, and can carry out rapid detection on clenbuterol hydrochloride.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to a biosensor sensitive film, and also relates to the application of the biosensor sensitive film in detecting clenbuterol hydrochloride. Background technique [0002] In recent years, with the frequent occurrence of emergencies caused by food safety issues, food safety issues have attracted widespread attention from all aspects of society. Rapid and sensitive detection of food pathogens (Salmonella, Campylobacter, Escherichia coli, etc.) and food contaminants in food has become a major problem for researchers. [0003] A biosensor is a sensor that uses immobilized biological components or the biological body itself as a sensitive element, and is an analytical test device that converts biochemical reaction energy into electrical signals. SPR biosensors are biosensors based on surface plasmon resonance (SPR) technology. SPR is very sensitive to the refractive index of the elect...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N21/552
CPCG01N33/54393
Inventor 张治红王要丽田志壮刘健张烁
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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