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A kind of extraction method of phycocyanin

A technology of phycocyanin and an extraction method, applied in the field of biomedicine, can solve the problems of long production cycle and large environmental pollution, and achieve the effects of improving production efficiency, high purity, and realizing the removal of macromolecular biological impurities

Active Publication Date: 2017-05-10
TAIZHOU BINMEI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of repeated freezing and thawing method has the problem of long production cycle, and the use of two-phase method has a greater pollution to the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take fresh spirulina algae mud, weigh it, and then add ice powder at -10°C according to the weight ratio of algae mud to ice powder at a ratio of 1:10. The particle size of the ice powder is 40-20 mesh. Add algae mud and ice powder into the mixing tank together, control the stirring speed at 1000r / min and control the system temperature below 0°C to keep warm for 80 minutes of wall breaking treatment. After the wall breaking treatment, make the ice powder in the system Naturally melted to obtain a wall-breaking solution containing phycocyanin. After testing, the wall-breaking rate of algae cells reached more than 95%;

[0032] Insert the cotton column made of medical absorbent cotton into the above-mentioned wall-breaking solution containing phycocyanin to make the phycocyanin adsorb on the cotton column. The medical absorbent cotton can be fixed with a stainless steel filter, and the height of the cotton column can be adjusted according to actual needs. Make adjustments...

Embodiment 2

[0036] Take the algae powder of spirulina and weigh it. It is best to pre-soak the above-mentioned algae powder and water according to the weight ratio of 1:3. Then add ice powder at -50°C according to the weight ratio of algae powder and ice powder at a ratio of 1:20. Put it into the stirring tank, control the stirring speed at 3000r / min and control the system temperature below -10°C to keep warm for 70 minutes for wall breaking treatment. After the wall breaking treatment, let the ice powder in the system melt naturally to obtain Phycocyanin wall-breaking solution, after testing, the wall-breaking rate of algae cells reaches more than 95%;

[0037] Insert the cotton column made of medical absorbent cotton into the above-mentioned wall-breaking solution containing phycocyanin to make the phycocyanin adsorb on the cotton column. The medical absorbent cotton can be fixed with a stainless steel filter, and the height of the cotton column can be adjusted according to actual needs...

Embodiment 3

[0041] To obtain the algae powder of cyanobacteria, it is best to pre-soak the algae powder of the above-mentioned cyanobacteria and water in a weight ratio of 1:3. Weigh, and then add ice powder at -30°C according to the weight ratio of algae powder and ice powder at a ratio of 1:15. The particle size of the ice powder is 40-20 mesh. Put it into the mixing tank together, control the stirring speed at 2000r / min and control the system temperature between 0°C and -20°C to keep warm for 90 minutes of wall breaking treatment. After the wall breaking treatment, the ice powder in the system will naturally Melted to obtain a wall-breaking solution containing phycocyanin. After testing, the wall-breaking rate of algae cells reached more than 95%;

[0042] Insert the cotton column made of medical absorbent cotton into the above-mentioned wall-breaking solution containing phycocyanin to make the phycocyanin adsorb on the cotton column. The medical absorbent cotton can be fixed with a st...

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Abstract

The invention relates to a phycocyanin extraction method, which belongs to the technical field of biological medicine. The invention provides the phycocyanin extraction method for solving the current problems of long wall breaking period and large environmental pollution. The method comprises the following steps: adding phycocyanin-containing fresh algae or pre-immersed algae powder in ice powder at -50 to -10 DEG C, controlling the stirring speed at 1000r / min-3000r / min for wall breaking treatment to obtain a wall breaking liquid containing phycocyanin; separating and purifying the wall breaking liquid containing the phycocyanin, and drying to obtain the finished product phycocyanin. The method has the advantages of short wall breaking period and long friendly environment, and the product has the advantage of high purity.

Description

technical field [0001] The invention relates to a method for extracting phycocyanin, belonging to the technical field of biomedicine. Background technique [0002] Phycocyanin is extracted from algal cells containing phycobilisomes. It is the only light-harvesting pigment protein in cyanobacteria. It is a very rare natural blue pigment and nutrient with unique medicinal efficacy and fluorescent properties. Among them, the cyanobacteria represented by spirulina is rich in phycocyanin, and has safety and high digestion and absorption rate. It has been certified by the Ministry of Health of China and the US Food and Drug Administration that spirulina is completely non-toxic and side effects. It is a safe plant drug and Health products. [0003] However, the existing cell disruption methods include natural swelling method, repeated freezing and thawing method, ultrasonic method and enzymatic hydrolysis method. The natural swelling method generally takes 10 to 20 days, and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/795C07K1/34
CPCC07K14/795
Inventor 张兵权
Owner TAIZHOU BINMEI BIOTECH
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