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Method for extracting total timosaponin

An extraction method and a technology of total saponins, which are applied in the field of extraction and purification of total timosaponins, can solve the problems that the technical literature and patents of total timosaponins are rarely seen, and achieve easy industrial production, improved purity, and low pollution Effect

Inactive Publication Date: 2014-12-03
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in my country, there are few technical literatures and patents related to the extraction of total timosaponins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take fresh Anemarrhena anemarrhena, dry, crush, and pass through a 40-mesh sieve. Weigh 50 grams of Anemarrhena powder and put it into an extractor, add 25 ml of water to soak for 30 minutes, then add 0.25 grams of pectinase reagent, and enzymolyze it for 2 minutes at a temperature of 30°C to collect the raw materials for enzymolysis. Then 250 ml of 60% ethanol aqueous solution was added for reflux extraction, the filter residue was extracted twice, the filtrates were combined three times, and concentrated under reduced pressure to 1 / 4 of the original volume to obtain the concentrated solution of total saponins of Anemarrhena. Pour the concentrated solution of total saponins of Anemarrhena into the X-5 macroporous adsorption resin column, first wash away the sugar with 2 times the column volume of water, then wash away some impurities with 2 times the column volume and 10% ethanol, and finally use 2 times the column volume volume ethanol for elution, the collected ethan...

Embodiment 2

[0024] Take fresh Anemarrhena, dry, crush, and pass through a 60-mesh sieve. Weigh 50 grams of Anemarrhena powder and put it into an extractor, add 30 ml of water to soak for 40 minutes, then add 1 gram of pectinase, and enzymolyze it for 3 minutes at a temperature of 40°C, collect the raw materials for enzymolysis, and then Add 250 ml of 60% ethanol aqueous solution for reflux extraction, extract the filter residue twice, combine the filtrates three times, concentrate under reduced pressure to 1 / 3 of the original volume, and obtain the concentrated solution of total saponins of Anemarrhena. Pour the concentrated solution of total saponins of Anemarrhena into a D3520 macroporous adsorption resin column, first wash off the sugar with 3 times the column volume of water, then wash away some impurities with 4 times the column volume and 15% ethanol, and finally wash it with 4 times the column volume of ethanol After elution, the collected ethanol eluate was concentrated under redu...

Embodiment 3

[0026] Take fresh Anemarrhena, dry, crush, and pass through a 60-mesh sieve. Weigh 50 grams of Anemarrhena powder and put it into an extractor, add 50 ml of water to soak for 60 minutes, then add 1.5 grams of biological enzyme reagent, and enzymolyze it for 4 minutes at a temperature of 45°C to collect the raw materials for enzymolysis. Then 250 ml of 60% ethanol aqueous solution was added for reflux extraction, the filter residue was extracted 3 times, the filtrates were combined 4 times, concentrated under reduced pressure to 1 / 2 of the original volume, and the concentrated solution of total saponins of Anemarrhena was obtained. Pour the concentrated solution of total saponins of Anemarrhena into the AB-8 macroporous adsorption resin column, first wash off the sugar with 4 times the column volume of water, then wash away some impurities with 5 times the column volume and 20% ethanol, and finally use 5 times the column volume volume ethanol for elution, collect the ethanol el...

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Abstract

The invention discloses a method for extracting total timosaponin. The method comprises the following steps: (1) drying and grinding rhizoma anemarrhenae; (2) weighing a certain amount of rhizoma anemarrhenae powder, adding water and infiltrating the powder, and adding a biological enzyme reagent which is 0.5-3 percent of the weight of the rhizoma anemarrhenae powder for performing enzymolysis; (3) adding an aqueous solution of ethanol and performing reflux extraction after enzymolysis is finished, and thus obtaining an extracting solution; (4) filtering the extracting solution, repeatedly extracting the filter residues, merging the extracting solutions, performing vacuum concentration, thereby obtaining a concentrated solution of the total timosaponin; and (5) eluting the concentrated solution on a macroporous resin column, collecting the ethanol eluent, performing vacuum concentration, and drying, thereby obtaining the total timosaponin. With the adoption of the method disclosed by the invention, the total timosaponin is prepared, the process is easy to operate, the production cost is low, the prepared product is high in purity, and industrial production is easily realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for extracting and purifying total timosaponins by combining biological enzymolysis technology and macroporous adsorption resin. Background technique [0002] Anemarrhena, the name of traditional Chinese medicine, belongs to the medicine for clearing away heat and lowering gunpowder. Indications: febrile disease, high fever and polydipsia, cough and asthma, dry cough, constipation, bone steaming and hot flashes, restlessness and insomnia, diarrhea and turbidity. Zhimu is also called Maozhimu. It is cultivated all over our country. It has a strong ability to resist drought and cold. It is a perennial herb that can grow in barren mountains, deserts and wastelands with little rain. The preferred variety, which can be sown year-round in all seasons, but mainly in spring or summer. Anemarrhena is a commonly used Chinese herbal medicine and is widely used by the society. Anemarrh...

Claims

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Application Information

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IPC IPC(8): A61K36/8964
Inventor 苏刘花杨存
Owner NANJING ZELANG AGRI DEV
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