Method for extracting L-lysine from molasses fermentation liquor
A technology of molasses fermentation broth and lysine, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., to achieve the effects of reducing production costs, wide range of adaptability, and reducing production energy consumption
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Embodiment 1
[0026] Example 1 This example illustrates the method for extracting L-lysine from molasses fermentation broth
[0027] The beet molasses fermentation broth with L-lysine concentration of 90 g / L was centrifuged at 2000 rpm for 5 min. , collect the centrifugate and solids separately; filter the filtrate after centrifugation with a filter membrane with a pore size of 0.45 μm, and collect the filter residue and filtrate respectively; collect the solids and filter residue obtained from solid-liquid separation and membrane filtration as the reaction substrate for biogas fermentation ; Use the mixed solution of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester and cyclohexane as the extractant, and the concentration of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester in the extractant is 1 mol / L , the extractant and the filtrate obtained by membrane filtration were mixed at a ratio of 1:1, the extraction temperature was 25 ° C, shaken for 4 minutes, and then separated into laye...
Embodiment 2
[0028] Embodiment 2 This embodiment illustrates the method for extracting L-lysine from molasses fermented liquid
[0029] The sugarcane molasses fermentation broth with L-lysine concentration of 140 g / L was centrifuged at 2000 rpm for 5 min. , collect the centrifugate and solids separately; filter the filtrate after centrifugation with a filter membrane with a pore size of 0.22 μm, and collect the filter residue and filtrate respectively; collect the solids and filter residue obtained from solid-liquid separation and membrane filtration as the reaction substrate for biogas fermentation ; Use the mixed solution of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester and cyclohexane as the extractant, and the concentration of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester in the extractant is 0.4 mol / L , the extractant and the filtrate obtained by membrane filtration were mixed at a ratio of 1:1, the extraction temperature was 25 °C, shaken for 2 min, and then separated i...
Embodiment 3
[0030] Embodiment 3 This embodiment illustrates the method for extracting L-lysine from molasses fermented liquid
[0031] Grape molasses fermentation broth with an L-lysine concentration of 250 g / L was centrifuged at 3000 rpm for 8 min. , collect the centrifugate and solids separately; filter the filtrate after centrifugation with a filter membrane with a pore size of 0.22 μm, and collect the filter residue and filtrate respectively; collect the solids and filter residue obtained from solid-liquid separation and membrane filtration as the reaction substrate for biogas fermentation ; Use the mixed solution of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester and cyclohexane as the extractant, and the concentration of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester in the extractant is 2 mol / L , the extractant was mixed with the filtrate obtained by membrane filtration at a ratio of 1:1, the extraction temperature was 25 °C, shaken for 5 min, and then separated in...
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