Method for extracting L-lysine from molasses fermentation liquor

A technology of molasses fermentation broth and lysine, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., to achieve the effects of reducing production costs, wide range of adaptability, and reducing production energy consumption

Active Publication Date: 2014-12-24
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the method of extracting high-concentration L-lysine from the very complex molasses fermented liquid at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 This example illustrates the method for extracting L-lysine from molasses fermentation broth

[0027] The beet molasses fermentation broth with L-lysine concentration of 90 g / L was centrifuged at 2000 rpm for 5 min. , collect the centrifugate and solids separately; filter the filtrate after centrifugation with a filter membrane with a pore size of 0.45 μm, and collect the filter residue and filtrate respectively; collect the solids and filter residue obtained from solid-liquid separation and membrane filtration as the reaction substrate for biogas fermentation ; Use the mixed solution of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester and cyclohexane as the extractant, and the concentration of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester in the extractant is 1 mol / L , the extractant and the filtrate obtained by membrane filtration were mixed at a ratio of 1:1, the extraction temperature was 25 ° C, shaken for 4 minutes, and then separated into laye...

Embodiment 2

[0028] Embodiment 2 This embodiment illustrates the method for extracting L-lysine from molasses fermented liquid

[0029] The sugarcane molasses fermentation broth with L-lysine concentration of 140 g / L was centrifuged at 2000 rpm for 5 min. , collect the centrifugate and solids separately; filter the filtrate after centrifugation with a filter membrane with a pore size of 0.22 μm, and collect the filter residue and filtrate respectively; collect the solids and filter residue obtained from solid-liquid separation and membrane filtration as the reaction substrate for biogas fermentation ; Use the mixed solution of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester and cyclohexane as the extractant, and the concentration of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester in the extractant is 0.4 mol / L , the extractant and the filtrate obtained by membrane filtration were mixed at a ratio of 1:1, the extraction temperature was 25 °C, shaken for 2 min, and then separated i...

Embodiment 3

[0030] Embodiment 3 This embodiment illustrates the method for extracting L-lysine from molasses fermented liquid

[0031] Grape molasses fermentation broth with an L-lysine concentration of 250 g / L was centrifuged at 3000 rpm for 8 min. , collect the centrifugate and solids separately; filter the filtrate after centrifugation with a filter membrane with a pore size of 0.22 μm, and collect the filter residue and filtrate respectively; collect the solids and filter residue obtained from solid-liquid separation and membrane filtration as the reaction substrate for biogas fermentation ; Use the mixed solution of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester and cyclohexane as the extractant, and the concentration of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester in the extractant is 2 mol / L , the extractant was mixed with the filtrate obtained by membrane filtration at a ratio of 1:1, the extraction temperature was 25 °C, shaken for 5 min, and then separated in...

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Abstract

The invention discloses a method for extracting L-lysine from a molasses fermentation liquor. The method is characterized by comprising solid-liquid separation, film filtration, solid recovery, chemical extraction and reverse extraction. The method specifically comprises the following steps: removing solid substances such as thallus and colloid out of molasses fermentation liquor, and then fully mixing with an extracting solution; oscillating at room temperature, extracting to reach balance, and then separating the extracting solution from raffinate; carrying out reverse extraction on the raffinate by using hydrochloric acid and then recovering an organic solvent; directly recovering the raffinate as a component of a fermentation medium, wherein filter residues separated from the fermentation liquor can be used as a substrate for methane fermentation. L-lysine is extracted at room temperature, so that the distribution ratio of high-concentration L-lysine is increased. A molasses pigment is completely separated from L-lysine, so that subsequent purification and rectification of L-lysine are facilitated. An organic solvent for extracting is completely recovered, and all the raffinate is recovered as a culture medium component, so that the process is environment-friendly and free of pollutant emission; the production cost is effectively reduced; the economical efficiency is improved; and good social benefits can also be brought about.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for extracting L-lysine from molasses fermentation liquid. Background technique [0002] The separation and extraction of amino acids in fermentation broth has always been a technical difficulty in the production process of amino acids. If lysine can be effectively separated from impurities such as sugar, sulfate, calcium ions, magnesium ions, other amino acids, and pigments in the fermentation broth, it will be Improve the purity of L-lysine products. [0003] The extraction and separation methods of amino acids include precipitation method, ion exchange method, reverse micellar extraction method, electrodialysis method, liquid membrane separation method, solvent extraction method and so on. The precipitation method has strong selectivity and simple operation, but in the production process, if a better separation effect needs to be achieved, it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C227/40C07C229/26
Inventor 陈可泉何珣肖文张弘欧阳平凯
Owner NANJING TECH UNIV
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