Preparation method and anti-tumor application of aciculatin

A technology of bamboo grass and bamboo joints, which is applied in the field of preparation of plant extracts and can solve problems such as no bamboo grass elements.

Inactive Publication Date: 2014-12-24
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Through literature search, no bamboo grass has b

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Preparation of Phyllostachys

[0017] After drying the raw material of bamboo joint grass rhizome, pulverize 60 meshes, take 5kg into the supercritical extraction kettle, pass in liquid CO2, set the extraction pressure 15MPa, temperature 50℃, after reaching the above parameters, adjust the liquid CO2 flow rate to 20kg / h, dynamic After 3 hours of extraction, analysis was performed under a pressure of 5 MPa and a temperature of 50° C. to obtain 120 g of extract. Disperse the extract with 2L hot water and add it to a resin column containing 500g AB-8 resin for adsorption. Take 4BV60% ethanol to elute impurities, and then use 5BV 90% ethanol solution to elute the effective components. Collect the eluent and concentrate under reduced pressure. Precipitate, add 85% methanol to the precipitate to dissolve under reflux, place for crystallization, filter, wash and dry to obtain panaxin with a content of 97.4%.

Embodiment 2

[0019] After drying, the raw material of bamboo joint grass rhizome is crushed to 80 mesh, 5kg is added to the supercritical extraction kettle, liquid CO2 is introduced, and the extraction pressure is 19MPa and the temperature is 60℃. After the above parameters are reached, the liquid CO2 flow rate is adjusted to 25kg / h, dynamic Extracted for 2 hours, analyzed under the pressure of 8MPa and temperature of 60°C to obtain 130g of extract. Disperse the extract with 2L hot water and add it to a resin column containing 500g HZ816 resin for adsorption, take 5BV50% ethanol to elute impurities, and then use 5BV 80% ethanol solution to elute the active ingredients, collect the eluate under reduced pressure and concentrate, and place it for precipitation , The precipitate is dissolved in 85% methanol under reflux, placed for crystallization, filtered, washed and dried to obtain panaxin, with a content of 96.7%.

Embodiment 3

[0021] After drying, the raw material of bamboo joint grass rhizome is crushed to 60 mesh, 5kg is added to the supercritical extraction kettle, and liquid CO2 is introduced. The extraction pressure is 25MPa and the temperature is 50℃. After the above parameters are reached, the flow rate of liquid CO2 is adjusted to 20kg / h. After 3 hours of extraction, analysis was performed under a pressure of 6 MPa and a temperature of 50° C. to obtain 110 g of extract. The extract was dispersed with 2L of hot water and added to a resin column containing 500g of D101 resin for adsorption, 6BV40% ethanol was taken to elute the impurities, and then 8BV75% ethanol solution was used to elute the active ingredients. The eluent was collected and concentrated under reduced pressure and placed for precipitation. The precipitate is dissolved in 85% methanol under reflux, placed for crystallization, filtered, washed and dried to obtain panaxalin with a content of 97.5%.

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Abstract

The invention discloses a preparation method and application of plant extracts, and in particular relates to a preparation method and anti-tumor application of aciculatin. The preparation method comprises the following steps: (1) crushing chrysopogon aciculatus roots and stems into 60-80 meshes, adding into a supercritical extraction kettle, introducing liquid CO2 to perform supercritical extraction for 2-3 hours under the conditions that the extraction pressure is 15-25MPa and the temperature is 40-60 DEG C, and performing analysis under the conditions that the pressure is 5-10MPa and the temperature is 50-60 DEG C to obtain an extract; (2) dispersing the extract by hot water, performing adsorption in macroporous resin, performing elution by using an ethanol water solution, collecting eluent, and concentrating the eluent to obtain precipitates; and (3) adding the precipitates into 85% methanol to perform reflux dissolution, standing and performing recrystallization, and drying to obtain the aciculatin. The preparation method disclosed by the invention is simple and convenient in process operation, high in yield and small in pollution, and is suitable for producing the high-purity aciculatin. The aciculatin disclosed by the invention has an anti-tumor effect, and an intestinal absorption promoter is added in an oral administration formula so as to facilitate the improvement of bioavailability.

Description

Technical field [0001] The invention discloses a preparation method and application of a plant extract, and particularly relates to a preparation method and anti-tumor application of panaxalin. Background technique [0002] Aciculatin, yellow crystal, molecular formula C 22 H 22 O 8 , With a molecular weight of 414.41, is a flavonoid glycoside compound extracted and isolated from the rhizome of bamboo joint grass. Pharmacological studies have shown that panaxan has cytotoxic activity on KB cells. Bamboo knotweed is the whole plant of Commelina Commelina plant, which has the effects of clearing heat and detoxification, diuresis and swelling, and hemostasis; main sores, carbuncle, sore throat, heat dysentery, turbidity, poor urination, traumatic bleeding, etc disease. [0003] Through literature search, there has not been any domestic use of panaxan for the preparation of anti-tumor drugs. Summary of the invention [0004] In order to meet clinical needs, expand the types of medici...

Claims

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Application Information

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IPC IPC(8): C07D493/18A61K31/365A61P35/00
CPCC07D493/18Y02P20/54
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
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