Method and system for magnetic micromanipulation of cells in physiological environment
A technique of physiological environment and micromanipulation, applied in biochemical equipment and methods, biomass post-processing, biological material pretreatment, etc., can solve unseen problems and achieve the effect of improving scanning efficiency and scanning accuracy
Active Publication Date: 2017-12-15
CHANGCHUN UNIV OF SCI & TECH
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Problems solved by technology
[0005] Since cells are basically non-magnetic, and it is difficult to manipulate biological cells in a physiological environment, there are no relevant reports on the application of micro-nano manipulation technology to the magnetic research of in vitro cells.
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[0010] As shown in Figure 1, the magnetic micromanipulation system for cells includes a magnetic probe, a probe nano-shift detection module, a three-dimensional displacement platform, an inverted / upright optical microscope, a magnetic probe control module, a displacement control module and an environment control module. system composition. The liquid pool is placed above the scanner, and a cover glass with cells is placed in the pool, and the piezoelectric ceramic tube can control the independent movement of the liquid pool in the X, Y, and Z directions under the action of an applied voltage. The laser beam emitted by the diode laser is focused on the back of the micro-cantilever by the optical system, and then reflected to the photodetector by the back of the micro-cantilever. Due to the interaction force between the atoms on the sample surface and the atoms at the tip of the micro-cantilever probe, when the sample is scanned, the micro-cantilever will bend and undulate with ...
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The invention discloses a cell magnetic force micromanipulation method and system in a physiological environment. The system for magnetic micromanipulation of cells in a physiological environment is a system composed of a magnetic probe, a probe nano-shift detection module, a three-dimensional displacement platform, an inverted / upright optical microscope, a magnetic probe control module and a displacement control module. In a controlled culture environment suitable for cell growth in vitro, the operation and real-time tracking and observation of biological cells in vitro are realized. The method of cell magnetic micromanipulation is based on the combination of cell magnetic micromanipulation and magnetic nanoparticles, using magnetic probes to measure and record the magnetic force point by point in selected areas of a single cell containing magnetic nanoparticles, and obtain simultaneously at the nanometer scale. Magnetic and topographic lattice images, precise positioning of magnetic cells and magnetic nanoparticles, and then use magnetic probes to operate, move, transport, inject and test individual cells, which has guiding significance for nanobiotechnology and micro-nano manipulation technology.
Description
technical field [0001] The invention relates to a method and system for micromanipulating cells by magnetic force in a physiological environment, and its potential applications include the following scientific and technological fields: biological science, medicine, electronics, computer science and the like. technical background [0002] Nanoscale magnetic measurement is one of the most important nanotechnology, and its application prospect is extremely broad, especially in the field of biomedicine research is of great significance. [0003] In 1990, IBM's Eigler and others used a scanning tunneling microscope to form the word "IBM" on the surface of nickel with a scanning tunneling microscope, thus realizing the transfer of atoms for the first time. In 1998, Stark et al. used atomic force microscope probes to image and cut DNA. In 2012, Kim et al. used atomic force microscopy to discover that the nature of cancer cell apoptosis is related to changes in surface roughness an...
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IPC IPC(8): C12N13/00C12M1/36C12M1/34
Inventor 曲英敏王作斌刘劲芸侯煜刘洋王国梁李文君翁占坤李景梅宋正勋胡贞许红梅于淼刘兰娇
Owner CHANGCHUN UNIV OF SCI & TECH